Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS01280 and ABZR87_RS01285 are separated by 105 nucleotides ABZR87_RS01285 and ABZR87_RS01290 are separated by 110 nucleotides
ABZR87_RS01280: ABZR87_RS01280 - tRNA guanosine(34) transglycosylase Tgt, at 266,818 to 267,969
_RS01280
ABZR87_RS01285: ABZR87_RS01285 - preprotein translocase subunit YajC, at 268,075 to 268,407
_RS01285
ABZR87_RS01290: ABZR87_RS01290 - protein translocase subunit SecD, at 268,518 to 270,407
_RS01290
Position (kb)
268
269 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 267.262 kb on + strand, within ABZR87_RS01280 at 267.263 kb on - strand, within ABZR87_RS01280 at 267.485 kb on - strand, within ABZR87_RS01280 at 267.580 kb on + strand, within ABZR87_RS01280 at 267.580 kb on + strand, within ABZR87_RS01280 at 267.581 kb on - strand, within ABZR87_RS01280 at 267.581 kb on - strand, within ABZR87_RS01280 at 267.581 kb on - strand, within ABZR87_RS01280 at 267.726 kb on - strand, within ABZR87_RS01280 at 267.769 kb on + strand, within ABZR87_RS01280 at 267.770 kb on - strand, within ABZR87_RS01280 at 267.797 kb on - strand, within ABZR87_RS01280 at 267.862 kb on + strand at 267.865 kb on + strand at 267.865 kb on + strand at 267.865 kb on + strand at 267.865 kb on + strand at 267.865 kb on + strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.866 kb on - strand at 267.868 kb on + strand at 267.868 kb on + strand at 267.869 kb on - strand at 267.968 kb on - strand at 267.968 kb on - strand at 267.968 kb on - strand at 267.968 kb on - strand at 267.968 kb on - strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.021 kb on + strand at 268.022 kb on - strand at 268.022 kb on - strand at 268.096 kb on + strand at 268.312 kb on + strand, within ABZR87_RS01285 at 268.312 kb on + strand, within ABZR87_RS01285 at 268.312 kb on + strand, within ABZR87_RS01285 at 268.405 kb on + strand at 268.470 kb on + strand at 268.470 kb on + strand at 268.470 kb on + strand at 268.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 267,262 + ABZR87_RS01280 0.39 +1.0 267,263 - ABZR87_RS01280 0.39 +0.6 267,485 - ABZR87_RS01280 0.58 +1.1 267,580 + ABZR87_RS01280 0.66 +0.4 267,580 + ABZR87_RS01280 0.66 +0.2 267,581 - ABZR87_RS01280 0.66 -1.2 267,581 - ABZR87_RS01280 0.66 -0.3 267,581 - ABZR87_RS01280 0.66 -1.4 267,726 - ABZR87_RS01280 0.79 +1.2 267,769 + ABZR87_RS01280 0.83 +2.4 267,770 - ABZR87_RS01280 0.83 -0.9 267,797 - ABZR87_RS01280 0.85 -0.1 267,862 + -0.1 267,865 + +0.2 267,865 + +2.8 267,865 + -0.1 267,865 + +1.0 267,865 + +1.2 267,866 - +1.4 267,866 - -0.2 267,866 - +0.6 267,866 - -0.4 267,866 - +0.6 267,866 - +0.6 267,866 - +1.8 267,866 - -1.1 267,868 + -1.1 267,868 + +0.1 267,869 - -0.2 267,968 - -0.6 267,968 - -0.5 267,968 - -0.6 267,968 - -0.5 267,968 - -0.6 268,021 + +0.1 268,021 + -0.6 268,021 + -0.4 268,021 + -0.8 268,021 + +0.2 268,021 + -0.2 268,021 + +0.5 268,021 + -0.3 268,021 + +0.5 268,022 - +0.7 268,022 - +0.2 268,096 + -2.2 268,312 + ABZR87_RS01285 0.71 -1.4 268,312 + ABZR87_RS01285 0.71 -2.6 268,312 + ABZR87_RS01285 0.71 -1.1 268,405 + -0.8 268,470 + -0.9 268,470 + -0.2 268,470 + +0.6 268,470 + -0.2
Or see this region's nucleotide sequence