Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00970 and ABZR87_RS00975 are separated by 135 nucleotides ABZR87_RS00975 and ABZR87_RS00980 are separated by 264 nucleotides
ABZR87_RS00970: ABZR87_RS00970 - molecular chaperone DnaJ, at 192,928 to 194,076
_RS00970
ABZR87_RS00975: ABZR87_RS00975 - molecular chaperone DnaK, at 194,212 to 196,164
_RS00975
ABZR87_RS00980: ABZR87_RS00980 - phospholipase A, at 196,429 to 197,724
_RS00980
Position (kb) 194
195
196
197 Strain fitness (log2 ratio) -2
-1
0
1
2 at 193.313 kb on + strand, within ABZR87_RS00970 at 193.313 kb on + strand, within ABZR87_RS00970 at 193.357 kb on - strand, within ABZR87_RS00970 at 194.118 kb on - strand at 194.181 kb on - strand at 194.181 kb on - strand at 194.181 kb on - strand at 194.185 kb on + strand at 194.213 kb on + strand at 194.213 kb on + strand at 194.213 kb on + strand at 194.213 kb on + strand at 194.214 kb on - strand at 194.214 kb on - strand at 194.256 kb on + strand at 194.354 kb on + strand at 194.355 kb on - strand at 195.584 kb on + strand, within ABZR87_RS00975 at 195.731 kb on + strand, within ABZR87_RS00975 at 196.205 kb on - strand at 196.205 kb on - strand at 196.205 kb on - strand at 196.205 kb on - strand at 196.314 kb on - strand at 196.314 kb on - strand at 196.378 kb on + strand at 196.378 kb on + strand at 196.378 kb on + strand at 196.378 kb on + strand at 196.378 kb on + strand at 196.379 kb on - strand at 196.379 kb on - strand at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.597 kb on + strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.598 kb on - strand, within ABZR87_RS00980 at 196.675 kb on - strand, within ABZR87_RS00980 at 196.729 kb on + strand, within ABZR87_RS00980 at 196.729 kb on + strand, within ABZR87_RS00980 at 196.731 kb on + strand, within ABZR87_RS00980 at 196.731 kb on + strand, within ABZR87_RS00980 at 196.731 kb on + strand, within ABZR87_RS00980 at 196.953 kb on + strand, within ABZR87_RS00980 at 196.954 kb on - strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.155 kb on + strand, within ABZR87_RS00980 at 197.156 kb on - strand, within ABZR87_RS00980 at 197.156 kb on - strand, within ABZR87_RS00980 at 197.156 kb on - strand, within ABZR87_RS00980 at 197.156 kb on - strand, within ABZR87_RS00980 at 197.156 kb on - strand, within ABZR87_RS00980
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 193,313 + ABZR87_RS00970 0.34 +1.8 193,313 + ABZR87_RS00970 0.34 -1.7 193,357 - ABZR87_RS00970 0.37 +0.3 194,118 - +0.4 194,181 - -0.2 194,181 - +0.1 194,181 - -0.4 194,185 + +0.0 194,213 + +1.1 194,213 + +0.4 194,213 + -0.4 194,213 + +0.6 194,214 - +0.2 194,214 - -0.2 194,256 + +1.0 194,354 + -0.4 194,355 - +0.8 195,584 + ABZR87_RS00975 0.70 +1.8 195,731 + ABZR87_RS00975 0.78 +0.8 196,205 - +1.7 196,205 - +0.2 196,205 - +0.5 196,205 - +0.6 196,314 - +0.8 196,314 - -1.2 196,378 + -1.6 196,378 + -1.2 196,378 + +0.8 196,378 + -0.5 196,378 + -0.7 196,379 - +0.4 196,379 - -0.5 196,597 + ABZR87_RS00980 0.13 -1.2 196,597 + ABZR87_RS00980 0.13 -0.5 196,597 + ABZR87_RS00980 0.13 -0.9 196,597 + ABZR87_RS00980 0.13 +0.7 196,597 + ABZR87_RS00980 0.13 +1.0 196,597 + ABZR87_RS00980 0.13 -0.5 196,597 + ABZR87_RS00980 0.13 -1.9 196,597 + ABZR87_RS00980 0.13 +0.1 196,597 + ABZR87_RS00980 0.13 +0.8 196,597 + ABZR87_RS00980 0.13 -0.8 196,598 - ABZR87_RS00980 0.13 +0.7 196,598 - ABZR87_RS00980 0.13 +0.4 196,598 - ABZR87_RS00980 0.13 -0.3 196,598 - ABZR87_RS00980 0.13 -0.0 196,598 - ABZR87_RS00980 0.13 +0.3 196,598 - ABZR87_RS00980 0.13 +0.4 196,598 - ABZR87_RS00980 0.13 +1.2 196,598 - ABZR87_RS00980 0.13 -0.3 196,598 - ABZR87_RS00980 0.13 +1.0 196,598 - ABZR87_RS00980 0.13 -1.2 196,598 - ABZR87_RS00980 0.13 +1.4 196,675 - ABZR87_RS00980 0.19 +0.1 196,729 + ABZR87_RS00980 0.23 +0.6 196,729 + ABZR87_RS00980 0.23 -2.3 196,731 + ABZR87_RS00980 0.23 +0.7 196,731 + ABZR87_RS00980 0.23 +1.2 196,731 + ABZR87_RS00980 0.23 +0.2 196,953 + ABZR87_RS00980 0.40 +0.1 196,954 - ABZR87_RS00980 0.41 -0.2 197,155 + ABZR87_RS00980 0.56 -0.4 197,155 + ABZR87_RS00980 0.56 +0.2 197,155 + ABZR87_RS00980 0.56 +0.2 197,155 + ABZR87_RS00980 0.56 +0.1 197,155 + ABZR87_RS00980 0.56 -0.1 197,155 + ABZR87_RS00980 0.56 -0.7 197,155 + ABZR87_RS00980 0.56 +0.2 197,155 + ABZR87_RS00980 0.56 +1.0 197,155 + ABZR87_RS00980 0.56 +0.2 197,156 - ABZR87_RS00980 0.56 +0.0 197,156 - ABZR87_RS00980 0.56 +0.6 197,156 - ABZR87_RS00980 0.56 +0.5 197,156 - ABZR87_RS00980 0.56 -0.8 197,156 - ABZR87_RS00980 0.56 +0.8
Or see this region's nucleotide sequence