Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00385 and ABZR87_RS00390 are separated by 66 nucleotides ABZR87_RS00390 and ABZR87_RS00395 are separated by 220 nucleotides
ABZR87_RS00385: ABZR87_RS00385 - response regulator transcription factor, at 78,537 to 79,202
_RS00385
ABZR87_RS00390: ABZR87_RS00390 - hybrid sensor histidine kinase/response regulator, at 79,269 to 81,101
_RS00390
ABZR87_RS00395: ABZR87_RS00395 - bifunctional riboflavin kinase/FAD synthetase, at 81,322 to 82,320
_RS00395
Position (kb)
79
80
81
82 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 78.325 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.326 kb on + strand at 78.327 kb on - strand at 78.327 kb on - strand at 78.327 kb on - strand at 78.327 kb on - strand at 78.327 kb on - strand at 78.327 kb on - strand at 78.327 kb on - strand at 78.425 kb on - strand at 78.453 kb on + strand at 78.454 kb on - strand at 78.454 kb on - strand at 78.454 kb on - strand at 78.497 kb on + strand at 78.497 kb on + strand at 78.497 kb on + strand at 78.498 kb on - strand at 78.498 kb on - strand at 78.498 kb on - strand at 78.532 kb on + strand at 78.533 kb on - strand at 78.533 kb on - strand at 78.533 kb on - strand at 78.533 kb on - strand at 78.554 kb on + strand at 78.555 kb on - strand at 78.941 kb on + strand, within ABZR87_RS00385 at 78.942 kb on - strand, within ABZR87_RS00385 at 79.267 kb on + strand at 79.267 kb on + strand at 79.273 kb on + strand at 79.274 kb on - strand at 79.366 kb on + strand at 79.366 kb on + strand at 79.367 kb on - strand at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.464 kb on + strand, within ABZR87_RS00390 at 79.465 kb on - strand, within ABZR87_RS00390 at 79.465 kb on - strand, within ABZR87_RS00390 at 79.674 kb on + strand, within ABZR87_RS00390 at 79.674 kb on + strand, within ABZR87_RS00390 at 79.675 kb on - strand, within ABZR87_RS00390 at 79.675 kb on - strand, within ABZR87_RS00390 at 79.675 kb on - strand, within ABZR87_RS00390 at 79.675 kb on - strand, within ABZR87_RS00390 at 79.675 kb on - strand, within ABZR87_RS00390 at 79.675 kb on - strand, within ABZR87_RS00390 at 79.829 kb on - strand, within ABZR87_RS00390 at 79.829 kb on - strand, within ABZR87_RS00390 at 79.829 kb on - strand, within ABZR87_RS00390 at 79.888 kb on + strand, within ABZR87_RS00390 at 79.888 kb on + strand, within ABZR87_RS00390 at 79.889 kb on - strand, within ABZR87_RS00390 at 79.889 kb on - strand, within ABZR87_RS00390 at 79.889 kb on - strand, within ABZR87_RS00390 at 79.891 kb on + strand, within ABZR87_RS00390 at 79.891 kb on + strand, within ABZR87_RS00390 at 79.891 kb on + strand, within ABZR87_RS00390 at 79.891 kb on + strand, within ABZR87_RS00390 at 79.891 kb on + strand, within ABZR87_RS00390 at 79.891 kb on + strand, within ABZR87_RS00390 at 79.892 kb on - strand, within ABZR87_RS00390 at 79.892 kb on - strand, within ABZR87_RS00390 at 79.892 kb on - strand, within ABZR87_RS00390 at 80.080 kb on - strand, within ABZR87_RS00390 at 80.323 kb on - strand, within ABZR87_RS00390 at 80.560 kb on + strand, within ABZR87_RS00390 at 80.560 kb on + strand, within ABZR87_RS00390 at 80.561 kb on - strand, within ABZR87_RS00390 at 80.737 kb on + strand, within ABZR87_RS00390 at 80.737 kb on + strand, within ABZR87_RS00390 at 80.737 kb on + strand, within ABZR87_RS00390 at 80.737 kb on + strand, within ABZR87_RS00390 at 80.737 kb on + strand, within ABZR87_RS00390 at 80.738 kb on - strand, within ABZR87_RS00390 at 80.738 kb on - strand, within ABZR87_RS00390 at 80.738 kb on - strand, within ABZR87_RS00390 at 80.738 kb on - strand, within ABZR87_RS00390 at 80.860 kb on + strand, within ABZR87_RS00390 at 80.861 kb on - strand, within ABZR87_RS00390 at 81.230 kb on + strand at 81.231 kb on - strand at 81.231 kb on - strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.232 kb on + strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.233 kb on - strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.234 kb on + strand at 81.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 78,325 + +0.6 78,326 + -1.2 78,326 + +0.3 78,326 + -0.2 78,326 + +1.1 78,326 + -1.5 78,326 + -0.5 78,326 + +0.5 78,326 + +0.5 78,326 + -0.2 78,326 + +1.2 78,326 + -0.8 78,326 + +0.6 78,326 + +2.4 78,327 - -2.8 78,327 - -0.0 78,327 - -0.1 78,327 - -1.1 78,327 - -0.7 78,327 - -0.6 78,327 - -0.8 78,425 - -1.2 78,453 + -1.0 78,454 - -0.7 78,454 - -0.3 78,454 - -0.6 78,497 + +0.6 78,497 + +0.4 78,497 + +0.4 78,498 - -1.2 78,498 - -0.3 78,498 - +0.8 78,532 + +0.6 78,533 - +0.0 78,533 - -0.7 78,533 - -0.6 78,533 - +0.3 78,554 + -0.3 78,555 - -0.6 78,941 + ABZR87_RS00385 0.61 -1.2 78,942 - ABZR87_RS00385 0.61 -1.4 79,267 + -1.2 79,267 + -0.8 79,273 + +2.4 79,274 - -0.1 79,366 + -1.7 79,366 + +0.2 79,367 - -1.4 79,464 + ABZR87_RS00390 0.11 -0.6 79,464 + ABZR87_RS00390 0.11 -0.9 79,464 + ABZR87_RS00390 0.11 -1.3 79,464 + ABZR87_RS00390 0.11 -1.6 79,464 + ABZR87_RS00390 0.11 -2.1 79,464 + ABZR87_RS00390 0.11 +1.3 79,464 + ABZR87_RS00390 0.11 +0.6 79,464 + ABZR87_RS00390 0.11 -0.5 79,465 - ABZR87_RS00390 0.11 -2.1 79,465 - ABZR87_RS00390 0.11 -0.5 79,674 + ABZR87_RS00390 0.22 -1.7 79,674 + ABZR87_RS00390 0.22 -1.8 79,675 - ABZR87_RS00390 0.22 +1.5 79,675 - ABZR87_RS00390 0.22 -0.4 79,675 - ABZR87_RS00390 0.22 +0.7 79,675 - ABZR87_RS00390 0.22 +0.2 79,675 - ABZR87_RS00390 0.22 +0.5 79,675 - ABZR87_RS00390 0.22 -1.0 79,829 - ABZR87_RS00390 0.31 -0.2 79,829 - ABZR87_RS00390 0.31 -0.5 79,829 - ABZR87_RS00390 0.31 -0.8 79,888 + ABZR87_RS00390 0.34 -0.7 79,888 + ABZR87_RS00390 0.34 -1.1 79,889 - ABZR87_RS00390 0.34 +0.0 79,889 - ABZR87_RS00390 0.34 -0.3 79,889 - ABZR87_RS00390 0.34 -0.4 79,891 + ABZR87_RS00390 0.34 -0.4 79,891 + ABZR87_RS00390 0.34 -0.8 79,891 + ABZR87_RS00390 0.34 +0.1 79,891 + ABZR87_RS00390 0.34 -1.3 79,891 + ABZR87_RS00390 0.34 +0.0 79,891 + ABZR87_RS00390 0.34 -1.1 79,892 - ABZR87_RS00390 0.34 -0.6 79,892 - ABZR87_RS00390 0.34 -0.5 79,892 - ABZR87_RS00390 0.34 -1.2 80,080 - ABZR87_RS00390 0.44 -2.1 80,323 - ABZR87_RS00390 0.58 -0.5 80,560 + ABZR87_RS00390 0.70 -1.6 80,560 + ABZR87_RS00390 0.70 -1.6 80,561 - ABZR87_RS00390 0.70 -0.6 80,737 + ABZR87_RS00390 0.80 -1.2 80,737 + ABZR87_RS00390 0.80 -0.7 80,737 + ABZR87_RS00390 0.80 -1.3 80,737 + ABZR87_RS00390 0.80 -0.9 80,737 + ABZR87_RS00390 0.80 -0.5 80,738 - ABZR87_RS00390 0.80 -0.3 80,738 - ABZR87_RS00390 0.80 -0.6 80,738 - ABZR87_RS00390 0.80 +0.1 80,738 - ABZR87_RS00390 0.80 -2.3 80,860 + ABZR87_RS00390 0.87 -1.5 80,861 - ABZR87_RS00390 0.87 -0.6 81,230 + -0.6 81,231 - +0.5 81,231 - -0.8 81,232 + +0.0 81,232 + -0.1 81,232 + -2.3 81,232 + -0.2 81,232 + -0.4 81,232 + -0.4 81,232 + +0.0 81,232 + +0.2 81,232 + -1.0 81,232 + -1.0 81,233 - +0.4 81,233 - +0.2 81,233 - -1.1 81,233 - -1.0 81,233 - -0.2 81,233 - +0.5 81,233 - -0.2 81,233 - -0.2 81,234 + -1.6 81,234 + +1.0 81,234 + -1.2 81,234 + +0.5 81,234 + -0.4 81,234 + +0.6 81,234 + +0.2 81,234 + -0.4 81,280 + -0.5
Or see this region's nucleotide sequence