Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00360 and ABZR87_RS00365 are separated by 370 nucleotides ABZR87_RS00365 and ABZR87_RS00370 are separated by 25 nucleotides ABZR87_RS00370 and ABZR87_RS00375 overlap by 4 nucleotides
ABZR87_RS00360: ABZR87_RS00360 - acyl-CoA desaturase, at 72,564 to 73,754
_RS00360
ABZR87_RS00365: ABZR87_RS00365 - mechanosensitive ion channel domain-containing protein, at 74,125 to 75,501
_RS00365
ABZR87_RS00370: ABZR87_RS00370 - phospholipase D family protein, at 75,527 to 76,096
_RS00370
ABZR87_RS00375: ABZR87_RS00375 - RsmB/NOP family class I SAM-dependent RNA methyltransferase, at 76,093 to 77,487
_RS00375
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 73.426 kb on + strand, within ABZR87_RS00360 at 73.427 kb on - strand, within ABZR87_RS00360 at 73.807 kb on - strand at 73.829 kb on - strand at 73.829 kb on - strand at 73.829 kb on - strand at 73.853 kb on - strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.935 kb on + strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.936 kb on - strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.937 kb on + strand at 73.938 kb on - strand at 73.938 kb on - strand at 73.938 kb on - strand at 73.938 kb on - strand at 73.938 kb on - strand at 73.959 kb on + strand at 73.959 kb on + strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.960 kb on - strand at 73.989 kb on + strand at 73.990 kb on - strand at 73.990 kb on - strand at 74.014 kb on + strand at 74.015 kb on - strand at 74.015 kb on - strand at 74.050 kb on + strand at 74.059 kb on + strand at 74.059 kb on + strand at 74.059 kb on + strand at 74.059 kb on + strand at 74.059 kb on + strand at 74.059 kb on + strand at 74.060 kb on - strand at 74.060 kb on - strand at 74.060 kb on - strand at 74.126 kb on + strand at 74.126 kb on + strand at 74.127 kb on - strand at 74.127 kb on - strand at 74.127 kb on - strand at 74.127 kb on - strand at 74.127 kb on - strand at 74.231 kb on + strand at 74.267 kb on + strand, within ABZR87_RS00365 at 74.268 kb on - strand, within ABZR87_RS00365 at 74.342 kb on + strand, within ABZR87_RS00365 at 74.343 kb on - strand, within ABZR87_RS00365 at 74.465 kb on + strand, within ABZR87_RS00365 at 74.466 kb on - strand, within ABZR87_RS00365 at 74.559 kb on + strand, within ABZR87_RS00365 at 74.560 kb on - strand, within ABZR87_RS00365 at 74.621 kb on + strand, within ABZR87_RS00365 at 74.621 kb on + strand, within ABZR87_RS00365 at 74.621 kb on + strand, within ABZR87_RS00365 at 74.621 kb on + strand, within ABZR87_RS00365 at 74.622 kb on - strand, within ABZR87_RS00365 at 74.622 kb on - strand, within ABZR87_RS00365 at 74.622 kb on - strand, within ABZR87_RS00365 at 74.622 kb on - strand, within ABZR87_RS00365 at 74.622 kb on - strand, within ABZR87_RS00365 at 74.690 kb on + strand, within ABZR87_RS00365 at 74.690 kb on + strand, within ABZR87_RS00365 at 74.690 kb on + strand, within ABZR87_RS00365 at 74.690 kb on + strand, within ABZR87_RS00365 at 74.691 kb on - strand, within ABZR87_RS00365 at 74.691 kb on - strand, within ABZR87_RS00365 at 74.691 kb on - strand, within ABZR87_RS00365 at 74.691 kb on - strand, within ABZR87_RS00365 at 75.011 kb on + strand, within ABZR87_RS00365 at 75.012 kb on - strand, within ABZR87_RS00365 at 75.012 kb on - strand, within ABZR87_RS00365 at 75.044 kb on + strand, within ABZR87_RS00365 at 75.044 kb on + strand, within ABZR87_RS00365 at 75.044 kb on + strand, within ABZR87_RS00365 at 75.045 kb on - strand, within ABZR87_RS00365 at 75.045 kb on - strand, within ABZR87_RS00365 at 75.045 kb on - strand, within ABZR87_RS00365 at 75.045 kb on - strand, within ABZR87_RS00365 at 75.045 kb on - strand, within ABZR87_RS00365 at 75.146 kb on + strand, within ABZR87_RS00365 at 75.147 kb on - strand, within ABZR87_RS00365 at 75.147 kb on - strand, within ABZR87_RS00365 at 75.147 kb on - strand, within ABZR87_RS00365 at 75.158 kb on + strand, within ABZR87_RS00365 at 75.158 kb on + strand, within ABZR87_RS00365 at 75.158 kb on + strand, within ABZR87_RS00365 at 75.158 kb on + strand, within ABZR87_RS00365 at 75.159 kb on - strand, within ABZR87_RS00365 at 75.159 kb on - strand, within ABZR87_RS00365 at 75.233 kb on + strand, within ABZR87_RS00365 at 75.233 kb on + strand, within ABZR87_RS00365 at 75.234 kb on - strand, within ABZR87_RS00365 at 75.234 kb on - strand, within ABZR87_RS00365 at 75.501 kb on + strand at 75.501 kb on + strand at 75.502 kb on - strand at 75.502 kb on - strand at 75.529 kb on - strand at 75.582 kb on + strand at 75.583 kb on - strand at 75.583 kb on - strand at 75.620 kb on + strand, within ABZR87_RS00370 at 75.620 kb on + strand, within ABZR87_RS00370 at 75.620 kb on + strand, within ABZR87_RS00370 at 75.621 kb on - strand, within ABZR87_RS00370 at 75.621 kb on - strand, within ABZR87_RS00370 at 75.621 kb on - strand, within ABZR87_RS00370 at 75.735 kb on + strand, within ABZR87_RS00370 at 75.736 kb on - strand, within ABZR87_RS00370 at 75.736 kb on - strand, within ABZR87_RS00370 at 75.736 kb on - strand, within ABZR87_RS00370 at 75.874 kb on + strand, within ABZR87_RS00370 at 75.875 kb on - strand, within ABZR87_RS00370 at 75.897 kb on + strand, within ABZR87_RS00370 at 75.897 kb on + strand, within ABZR87_RS00370 at 75.898 kb on - strand, within ABZR87_RS00370 at 75.898 kb on - strand, within ABZR87_RS00370 at 76.151 kb on + strand at 76.151 kb on + strand at 76.163 kb on + strand at 76.164 kb on - strand at 76.298 kb on + strand, within ABZR87_RS00375 at 76.299 kb on - strand, within ABZR87_RS00375
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 73,426 + ABZR87_RS00360 0.72 +0.3 73,427 - ABZR87_RS00360 0.72 +0.2 73,807 - +0.0 73,829 - -0.5 73,829 - +0.5 73,829 - +1.3 73,853 - -0.2 73,935 + +0.8 73,935 + -0.2 73,935 + +2.8 73,935 + +1.8 73,935 + -0.2 73,935 + +0.2 73,935 + +1.8 73,935 + -1.2 73,935 + +1.8 73,935 + -0.2 73,935 + +0.8 73,936 - +0.3 73,936 - -0.7 73,936 - +0.1 73,936 - +0.0 73,936 - -0.8 73,936 - -0.5 73,936 - +0.5 73,936 - +0.5 73,936 - +0.0 73,936 - -0.4 73,936 - +0.0 73,936 - +0.1 73,936 - +0.8 73,936 - -0.5 73,936 - +0.2 73,937 + +2.8 73,937 + -0.5 73,937 + +1.8 73,937 + +2.8 73,937 + +0.8 73,937 + -1.5 73,937 + +0.8 73,937 + +0.2 73,938 - -0.2 73,938 - +0.2 73,938 - +0.2 73,938 - +0.6 73,938 - +0.7 73,959 + +0.8 73,959 + +0.5 73,960 - +0.6 73,960 - -0.0 73,960 - -0.7 73,960 - +0.1 73,960 - +1.2 73,960 - -0.2 73,960 - -0.1 73,960 - -0.2 73,960 - +0.7 73,960 - -1.8 73,989 + +0.8 73,990 - +0.2 73,990 - -1.0 74,014 + -0.2 74,015 - -0.3 74,015 - +0.2 74,050 + -1.5 74,059 + +0.7 74,059 + +0.2 74,059 + +2.1 74,059 + +0.6 74,059 + +1.2 74,059 + +0.8 74,060 - -0.5 74,060 - -0.9 74,060 - -0.4 74,126 + +0.4 74,126 + -0.5 74,127 - +0.8 74,127 - -0.2 74,127 - +0.8 74,127 - +0.8 74,127 - -0.4 74,231 + +0.1 74,267 + ABZR87_RS00365 0.10 -0.8 74,268 - ABZR87_RS00365 0.10 -0.1 74,342 + ABZR87_RS00365 0.16 +0.0 74,343 - ABZR87_RS00365 0.16 -0.3 74,465 + ABZR87_RS00365 0.25 +0.0 74,466 - ABZR87_RS00365 0.25 -0.2 74,559 + ABZR87_RS00365 0.32 +0.2 74,560 - ABZR87_RS00365 0.32 +0.3 74,621 + ABZR87_RS00365 0.36 -1.7 74,621 + ABZR87_RS00365 0.36 +0.8 74,621 + ABZR87_RS00365 0.36 +1.0 74,621 + ABZR87_RS00365 0.36 +0.6 74,622 - ABZR87_RS00365 0.36 +0.3 74,622 - ABZR87_RS00365 0.36 +0.0 74,622 - ABZR87_RS00365 0.36 +0.0 74,622 - ABZR87_RS00365 0.36 +0.3 74,622 - ABZR87_RS00365 0.36 -0.5 74,690 + ABZR87_RS00365 0.41 +1.0 74,690 + ABZR87_RS00365 0.41 -0.4 74,690 + ABZR87_RS00365 0.41 +0.2 74,690 + ABZR87_RS00365 0.41 -0.1 74,691 - ABZR87_RS00365 0.41 -0.8 74,691 - ABZR87_RS00365 0.41 +1.3 74,691 - ABZR87_RS00365 0.41 +0.4 74,691 - ABZR87_RS00365 0.41 +0.2 75,011 + ABZR87_RS00365 0.64 +1.2 75,012 - ABZR87_RS00365 0.64 +0.5 75,012 - ABZR87_RS00365 0.64 -0.2 75,044 + ABZR87_RS00365 0.67 -1.7 75,044 + ABZR87_RS00365 0.67 -0.6 75,044 + ABZR87_RS00365 0.67 -1.6 75,045 - ABZR87_RS00365 0.67 -0.1 75,045 - ABZR87_RS00365 0.67 -1.0 75,045 - ABZR87_RS00365 0.67 -0.4 75,045 - ABZR87_RS00365 0.67 +0.2 75,045 - ABZR87_RS00365 0.67 -0.3 75,146 + ABZR87_RS00365 0.74 -3.2 75,147 - ABZR87_RS00365 0.74 -0.0 75,147 - ABZR87_RS00365 0.74 +0.3 75,147 - ABZR87_RS00365 0.74 -1.1 75,158 + ABZR87_RS00365 0.75 +0.1 75,158 + ABZR87_RS00365 0.75 -0.4 75,158 + ABZR87_RS00365 0.75 -1.5 75,158 + ABZR87_RS00365 0.75 +1.2 75,159 - ABZR87_RS00365 0.75 -0.0 75,159 - ABZR87_RS00365 0.75 -0.2 75,233 + ABZR87_RS00365 0.80 +0.5 75,233 + ABZR87_RS00365 0.80 -0.4 75,234 - ABZR87_RS00365 0.81 +0.6 75,234 - ABZR87_RS00365 0.81 +1.0 75,501 + -0.4 75,501 + -0.3 75,502 - -0.5 75,502 - -0.4 75,529 - +0.7 75,582 + +0.6 75,583 - -0.7 75,583 - +0.4 75,620 + ABZR87_RS00370 0.16 -0.3 75,620 + ABZR87_RS00370 0.16 +0.5 75,620 + ABZR87_RS00370 0.16 -0.4 75,621 - ABZR87_RS00370 0.16 -0.2 75,621 - ABZR87_RS00370 0.16 -0.2 75,621 - ABZR87_RS00370 0.16 +1.7 75,735 + ABZR87_RS00370 0.36 -1.0 75,736 - ABZR87_RS00370 0.37 -0.6 75,736 - ABZR87_RS00370 0.37 -0.2 75,736 - ABZR87_RS00370 0.37 +0.6 75,874 + ABZR87_RS00370 0.61 +1.2 75,875 - ABZR87_RS00370 0.61 -0.8 75,897 + ABZR87_RS00370 0.65 -1.2 75,897 + ABZR87_RS00370 0.65 +0.1 75,898 - ABZR87_RS00370 0.65 +0.4 75,898 - ABZR87_RS00370 0.65 -1.1 76,151 + -0.8 76,151 + -0.0 76,163 + -0.6 76,164 - -0.7 76,298 + ABZR87_RS00375 0.15 +0.3 76,299 - ABZR87_RS00375 0.15 -0.2
Or see this region's nucleotide sequence