Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26680

Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26675 and BWI76_RS26680 are separated by 185 nucleotidesBWI76_RS26680 and BWI76_RS26685 are separated by 71 nucleotides BWI76_RS26675: BWI76_RS26675 - phosphoesterase PA-phosphatase-like protein, at 5,523,043 to 5,524,314 _RS26675 BWI76_RS26680: BWI76_RS26680 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 5,524,500 to 5,525,342 _RS26680 BWI76_RS26685: BWI76_RS26685 - glutathione-disulfide reductase, at 5,525,414 to 5,526,766 _RS26685 Position (kb) 5524 5525 5526Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5523.526 kb on + strand, within BWI76_RS26675at 5523.608 kb on + strand, within BWI76_RS26675at 5523.647 kb on + strand, within BWI76_RS26675at 5523.647 kb on + strand, within BWI76_RS26675at 5523.647 kb on + strand, within BWI76_RS26675at 5523.652 kb on + strand, within BWI76_RS26675at 5523.655 kb on - strand, within BWI76_RS26675at 5523.655 kb on - strand, within BWI76_RS26675at 5523.698 kb on + strand, within BWI76_RS26675at 5523.698 kb on + strand, within BWI76_RS26675at 5523.706 kb on - strand, within BWI76_RS26675at 5523.762 kb on + strand, within BWI76_RS26675at 5523.820 kb on + strand, within BWI76_RS26675at 5523.828 kb on - strand, within BWI76_RS26675at 5523.916 kb on + strand, within BWI76_RS26675at 5523.921 kb on - strand, within BWI76_RS26675at 5523.997 kb on + strand, within BWI76_RS26675at 5524.214 kb on + strandat 5524.397 kb on - strandat 5524.432 kb on + strandat 5524.442 kb on + strandat 5524.445 kb on - strandat 5524.512 kb on + strandat 5524.515 kb on - strandat 5524.538 kb on - strandat 5524.586 kb on - strand, within BWI76_RS26680at 5524.633 kb on - strand, within BWI76_RS26680at 5524.719 kb on - strand, within BWI76_RS26680at 5524.760 kb on + strand, within BWI76_RS26680at 5524.784 kb on + strand, within BWI76_RS26680at 5524.827 kb on + strand, within BWI76_RS26680at 5524.889 kb on + strand, within BWI76_RS26680at 5524.938 kb on - strand, within BWI76_RS26680at 5524.953 kb on - strand, within BWI76_RS26680at 5525.527 kb on + strandat 5525.758 kb on - strand, within BWI76_RS26685at 5525.845 kb on - strand, within BWI76_RS26685at 5525.882 kb on - strand, within BWI76_RS26685at 5526.091 kb on - strand, within BWI76_RS26685at 5526.159 kb on + strand, within BWI76_RS26685at 5526.170 kb on + strand, within BWI76_RS26685at 5526.178 kb on - strand, within BWI76_RS26685

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
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5,523,526 + BWI76_RS26675 0.38 -0.2
5,523,608 + BWI76_RS26675 0.44 +0.4
5,523,647 + BWI76_RS26675 0.47 +0.2
5,523,647 + BWI76_RS26675 0.47 +1.8
5,523,647 + BWI76_RS26675 0.47 -0.1
5,523,652 + BWI76_RS26675 0.48 +0.2
5,523,655 - BWI76_RS26675 0.48 +0.1
5,523,655 - BWI76_RS26675 0.48 +0.5
5,523,698 + BWI76_RS26675 0.51 -0.1
5,523,698 + BWI76_RS26675 0.51 +1.6
5,523,706 - BWI76_RS26675 0.52 -2.4
5,523,762 + BWI76_RS26675 0.57 +0.3
5,523,820 + BWI76_RS26675 0.61 -0.1
5,523,828 - BWI76_RS26675 0.62 -0.3
5,523,916 + BWI76_RS26675 0.69 -1.9
5,523,921 - BWI76_RS26675 0.69 -2.9
5,523,997 + BWI76_RS26675 0.75 -0.5
5,524,214 + -0.2
5,524,397 - +0.5
5,524,432 + -0.2
5,524,442 + +0.5
5,524,445 - -0.7
5,524,512 + -1.1
5,524,515 - -0.7
5,524,538 - -1.7
5,524,586 - BWI76_RS26680 0.10 +0.0
5,524,633 - BWI76_RS26680 0.16 +0.0
5,524,719 - BWI76_RS26680 0.26 +0.6
5,524,760 + BWI76_RS26680 0.31 +0.4
5,524,784 + BWI76_RS26680 0.34 +1.1
5,524,827 + BWI76_RS26680 0.39 -1.7
5,524,889 + BWI76_RS26680 0.46 -0.1
5,524,938 - BWI76_RS26680 0.52 +1.9
5,524,953 - BWI76_RS26680 0.54 +2.2
5,525,527 + -2.8
5,525,758 - BWI76_RS26685 0.25 -0.7
5,525,845 - BWI76_RS26685 0.32 -2.1
5,525,882 - BWI76_RS26685 0.35 +0.2
5,526,091 - BWI76_RS26685 0.50 -0.5
5,526,159 + BWI76_RS26685 0.55 +1.1
5,526,170 + BWI76_RS26685 0.56 +1.2
5,526,178 - BWI76_RS26685 0.56 -0.1

Or see this region's nucleotide sequence