Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25405 and BWI76_RS25410 are separated by 210 nucleotides BWI76_RS25410 and BWI76_RS25415 overlap by 29 nucleotides BWI76_RS25415 and BWI76_RS25420 are separated by 131 nucleotides
BWI76_RS25405: BWI76_RS25405 - anion transporter, at 5,280,341 to 5,281,609
_RS25405
BWI76_RS25410: BWI76_RS25410 - GntR family transcriptional regulator with, at 5,281,820 to 5,282,485
_RS25410
BWI76_RS25415: BWI76_RS25415 - GntR family transcriptional regulator, at 5,282,457 to 5,283,101
_RS25415
BWI76_RS25420: BWI76_RS25420 - malate dehydrogenase, at 5,283,233 to 5,284,171
_RS25420
Position (kb) 5282
5283
5284 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5281.519 kb on + strand at 5281.908 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5281.984 kb on + strand, within BWI76_RS25410 at 5282.076 kb on + strand, within BWI76_RS25410 at 5282.103 kb on - strand, within BWI76_RS25410 at 5282.141 kb on + strand, within BWI76_RS25410 at 5282.141 kb on - strand, within BWI76_RS25410 at 5282.209 kb on - strand, within BWI76_RS25410 at 5282.209 kb on - strand, within BWI76_RS25410 at 5282.212 kb on + strand, within BWI76_RS25410 at 5282.301 kb on - strand, within BWI76_RS25410 at 5282.347 kb on - strand, within BWI76_RS25410 at 5282.351 kb on - strand, within BWI76_RS25410 at 5282.365 kb on + strand at 5282.477 kb on - strand at 5282.493 kb on - strand at 5282.875 kb on + strand, within BWI76_RS25415 at 5283.028 kb on - strand, within BWI76_RS25415 at 5283.192 kb on - strand at 5283.383 kb on + strand, within BWI76_RS25420 at 5283.633 kb on + strand, within BWI76_RS25420 at 5283.826 kb on + strand, within BWI76_RS25420 at 5283.844 kb on - strand, within BWI76_RS25420 at 5283.856 kb on - strand, within BWI76_RS25420 at 5284.085 kb on + strand at 5284.092 kb on - strand at 5284.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,281,519 + +0.4 5,281,908 + BWI76_RS25410 0.13 +0.0 5,281,984 + BWI76_RS25410 0.25 -3.0 5,281,984 + BWI76_RS25410 0.25 -0.9 5,281,984 + BWI76_RS25410 0.25 +1.0 5,282,076 + BWI76_RS25410 0.38 +0.1 5,282,103 - BWI76_RS25410 0.42 +1.0 5,282,141 + BWI76_RS25410 0.48 -0.2 5,282,141 - BWI76_RS25410 0.48 +0.1 5,282,209 - BWI76_RS25410 0.58 +0.2 5,282,209 - BWI76_RS25410 0.58 -1.7 5,282,212 + BWI76_RS25410 0.59 +0.2 5,282,301 - BWI76_RS25410 0.72 -0.2 5,282,347 - BWI76_RS25410 0.79 -0.2 5,282,351 - BWI76_RS25410 0.80 +0.5 5,282,365 + +0.9 5,282,477 - -0.0 5,282,493 - -1.4 5,282,875 + BWI76_RS25415 0.65 +0.9 5,283,028 - BWI76_RS25415 0.89 +0.6 5,283,192 - -0.7 5,283,383 + BWI76_RS25420 0.16 -2.3 5,283,633 + BWI76_RS25420 0.43 -0.6 5,283,826 + BWI76_RS25420 0.63 +0.7 5,283,844 - BWI76_RS25420 0.65 +0.4 5,283,856 - BWI76_RS25420 0.66 -0.0 5,284,085 + +0.5 5,284,092 - +0.4 5,284,092 - +0.0
Or see this region's nucleotide sequence