Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19765 and BWI76_RS19770 are separated by 426 nucleotides BWI76_RS19770 and BWI76_RS19775 are separated by 190 nucleotides
BWI76_RS19765: BWI76_RS19765 - membrane protein, at 4,132,161 to 4,132,868
_RS19765
BWI76_RS19770: BWI76_RS19770 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 4,133,295 to 4,133,864
_RS19770
BWI76_RS19775: BWI76_RS19775 - phage resistance protein, at 4,134,055 to 4,135,617
_RS19775
Position (kb)
4133
4134 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4132.295 kb on + strand, within BWI76_RS19765 at 4132.314 kb on + strand, within BWI76_RS19765 at 4132.374 kb on + strand, within BWI76_RS19765 at 4132.382 kb on - strand, within BWI76_RS19765 at 4132.446 kb on + strand, within BWI76_RS19765 at 4132.537 kb on - strand, within BWI76_RS19765 at 4132.585 kb on + strand, within BWI76_RS19765 at 4132.729 kb on - strand, within BWI76_RS19765 at 4132.775 kb on - strand, within BWI76_RS19765 at 4132.792 kb on - strand, within BWI76_RS19765 at 4132.810 kb on + strand at 4132.900 kb on + strand at 4133.020 kb on - strand at 4133.023 kb on + strand at 4133.124 kb on - strand at 4133.124 kb on - strand at 4133.124 kb on - strand at 4133.281 kb on + strand at 4133.289 kb on - strand at 4133.289 kb on - strand at 4133.358 kb on + strand, within BWI76_RS19770 at 4133.366 kb on - strand, within BWI76_RS19770 at 4133.847 kb on + strand at 4134.051 kb on + strand at 4134.059 kb on - strand at 4134.202 kb on + strand at 4134.202 kb on + strand at 4134.202 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,132,295 + BWI76_RS19765 0.19 +0.5 4,132,314 + BWI76_RS19765 0.22 -1.4 4,132,374 + BWI76_RS19765 0.30 +0.4 4,132,382 - BWI76_RS19765 0.31 +0.6 4,132,446 + BWI76_RS19765 0.40 +0.2 4,132,537 - BWI76_RS19765 0.53 -0.4 4,132,585 + BWI76_RS19765 0.60 -0.2 4,132,729 - BWI76_RS19765 0.80 +0.1 4,132,775 - BWI76_RS19765 0.87 -0.4 4,132,792 - BWI76_RS19765 0.89 +0.5 4,132,810 + -1.4 4,132,900 + -0.2 4,133,020 - -0.0 4,133,023 + -0.0 4,133,124 - -0.0 4,133,124 - +0.2 4,133,124 - -0.2 4,133,281 + -1.5 4,133,289 - -2.6 4,133,289 - -3.8 4,133,358 + BWI76_RS19770 0.11 -3.0 4,133,366 - BWI76_RS19770 0.12 -2.0 4,133,847 + -1.0 4,134,051 + +0.2 4,134,059 - +0.4 4,134,202 + -0.0 4,134,202 + -0.1 4,134,202 + +1.0
Or see this region's nucleotide sequence