Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18065 and BWI76_RS18070 are separated by 78 nucleotides BWI76_RS18070 and BWI76_RS18075 are separated by 6 nucleotides BWI76_RS18075 and BWI76_RS18080 are separated by 133 nucleotides BWI76_RS18080 and BWI76_RS18085 are separated by 144 nucleotides
BWI76_RS18065: BWI76_RS18065 - DNA polymerase III subunit theta, at 3,753,460 to 3,753,690
_RS18065
BWI76_RS18070: BWI76_RS18070 - DNA polymerase III subunit epsilon, at 3,753,769 to 3,754,428
_RS18070
BWI76_RS18075: BWI76_RS18075 - oligopeptidase B, at 3,754,435 to 3,756,495
_RS18075
BWI76_RS18080: BWI76_RS18080 - protein YebE, at 3,756,629 to 3,757,309
_RS18080
BWI76_RS18085: BWI76_RS18085 - hypothetical protein, at 3,757,454 to 3,757,816
_RS18085
Position (kb)
3754
3755
3756
3757 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3753.536 kb on + strand, within BWI76_RS18065 at 3753.590 kb on + strand at 3754.286 kb on + strand, within BWI76_RS18070 at 3754.287 kb on - strand, within BWI76_RS18070 at 3754.293 kb on - strand, within BWI76_RS18070 at 3754.385 kb on + strand at 3754.385 kb on + strand at 3754.447 kb on - strand at 3754.661 kb on - strand, within BWI76_RS18075 at 3754.942 kb on - strand, within BWI76_RS18075 at 3755.332 kb on - strand, within BWI76_RS18075 at 3755.332 kb on - strand, within BWI76_RS18075 at 3755.529 kb on + strand, within BWI76_RS18075 at 3755.856 kb on + strand, within BWI76_RS18075 at 3756.276 kb on - strand, within BWI76_RS18075 at 3756.525 kb on - strand at 3756.544 kb on + strand at 3756.562 kb on - strand at 3756.570 kb on - strand at 3756.626 kb on + strand at 3756.824 kb on + strand, within BWI76_RS18080 at 3757.080 kb on + strand, within BWI76_RS18080
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,753,536 + BWI76_RS18065 0.33 +0.9 3,753,590 + -0.6 3,754,286 + BWI76_RS18070 0.78 -2.2 3,754,287 - BWI76_RS18070 0.78 -1.3 3,754,293 - BWI76_RS18070 0.79 +0.8 3,754,385 + +0.4 3,754,385 + -2.7 3,754,447 - +0.3 3,754,661 - BWI76_RS18075 0.11 +2.0 3,754,942 - BWI76_RS18075 0.25 +0.4 3,755,332 - BWI76_RS18075 0.44 +0.4 3,755,332 - BWI76_RS18075 0.44 +1.0 3,755,529 + BWI76_RS18075 0.53 +0.9 3,755,856 + BWI76_RS18075 0.69 +1.0 3,756,276 - BWI76_RS18075 0.89 -0.6 3,756,525 - +0.7 3,756,544 + +0.2 3,756,562 - +1.2 3,756,570 - +1.2 3,756,626 + +0.2 3,756,824 + BWI76_RS18080 0.29 +0.1 3,757,080 + BWI76_RS18080 0.66 +0.6
Or see this region's nucleotide sequence