Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15965 and BWI76_RS15970 are separated by 419 nucleotides BWI76_RS15970 and BWI76_RS15975 are separated by 176 nucleotides BWI76_RS15975 and BWI76_RS15980 are separated by 149 nucleotides
BWI76_RS15965: BWI76_RS15965 - MFS transporter, at 3,311,408 to 3,312,658
_RS15965
BWI76_RS15970: BWI76_RS15970 - carboxypeptidase M32, at 3,313,078 to 3,314,565
_RS15970
BWI76_RS15975: BWI76_RS15975 - hypothetical protein, at 3,314,742 to 3,315,167
_RS15975
BWI76_RS15980: BWI76_RS15980 - lysine transporter LysE, at 3,315,317 to 3,315,579
_RS15980
BWI76_RS15985: BWI76_RS15985 - LysR family transcriptional regulator, at 3,315,560 to 3,315,784
_RS15985
Position (kb)
3313
3314
3315 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3312.323 kb on + strand at 3312.842 kb on - strand at 3313.022 kb on + strand at 3313.144 kb on - strand at 3313.493 kb on - strand, within BWI76_RS15970 at 3314.303 kb on - strand, within BWI76_RS15970 at 3314.645 kb on + strand at 3314.730 kb on + strand at 3314.782 kb on - strand at 3314.982 kb on + strand, within BWI76_RS15975 at 3315.261 kb on - strand at 3315.433 kb on - strand, within BWI76_RS15980
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,312,323 + +1.2 3,312,842 - -2.8 3,313,022 + +0.0 3,313,144 - +0.8 3,313,493 - BWI76_RS15970 0.28 +1.1 3,314,303 - BWI76_RS15970 0.82 +0.9 3,314,645 + -0.2 3,314,730 + -0.2 3,314,782 - -0.9 3,314,982 + BWI76_RS15975 0.56 +0.8 3,315,261 - +1.6 3,315,433 - BWI76_RS15980 0.44 +0.3
Or see this region's nucleotide sequence