Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15345

Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15340 and BWI76_RS15345 are separated by 176 nucleotidesBWI76_RS15345 and BWI76_RS15350 are separated by 149 nucleotidesBWI76_RS15350 and BWI76_RS15355 are separated by 67 nucleotides BWI76_RS15340: BWI76_RS15340 - urea ABC transporter substrate-binding protein, at 3,212,576 to 3,213,847 _RS15340 BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127 _RS15345 BWI76_RS15350: BWI76_RS15350 - hypothetical protein, at 3,215,277 to 3,215,669 _RS15350 BWI76_RS15355: BWI76_RS15355 - transcriptional regulator FtrA, at 3,215,737 to 3,216,747 _RS15355 Position (kb) 3214 3215 3216Strain fitness (log2 ratio) -2 -1 0 1at 3213.110 kb on - strand, within BWI76_RS15340at 3213.476 kb on - strand, within BWI76_RS15340at 3213.488 kb on - strand, within BWI76_RS15340at 3213.794 kb on + strandat 3213.795 kb on - strandat 3213.797 kb on + strandat 3213.856 kb on + strandat 3213.856 kb on + strandat 3213.856 kb on + strandat 3213.864 kb on - strandat 3213.864 kb on - strandat 3213.957 kb on - strandat 3214.012 kb on + strandat 3214.033 kb on - strandat 3214.040 kb on - strandat 3214.159 kb on - strand, within BWI76_RS15345at 3214.451 kb on + strand, within BWI76_RS15345at 3214.489 kb on + strand, within BWI76_RS15345at 3214.554 kb on - strand, within BWI76_RS15345at 3214.844 kb on - strand, within BWI76_RS15345at 3215.104 kb on + strandat 3215.295 kb on + strandat 3215.297 kb on - strandat 3215.303 kb on - strandat 3215.354 kb on - strand, within BWI76_RS15350at 3216.042 kb on + strand, within BWI76_RS15355at 3216.052 kb on + strand, within BWI76_RS15355

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
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3,213,110 - BWI76_RS15340 0.42 +0.1
3,213,476 - BWI76_RS15340 0.71 +0.9
3,213,488 - BWI76_RS15340 0.72 +1.5
3,213,794 + +0.6
3,213,795 - +0.4
3,213,797 + +0.3
3,213,856 + +0.5
3,213,856 + +1.0
3,213,856 + +1.2
3,213,864 - +0.5
3,213,864 - +0.5
3,213,957 - +0.3
3,214,012 + -1.9
3,214,033 - -0.2
3,214,040 - +0.3
3,214,159 - BWI76_RS15345 0.12 -0.4
3,214,451 + BWI76_RS15345 0.39 +0.3
3,214,489 + BWI76_RS15345 0.42 +1.0
3,214,554 - BWI76_RS15345 0.48 -1.0
3,214,844 - BWI76_RS15345 0.74 +0.5
3,215,104 + +0.1
3,215,295 + -1.0
3,215,297 - +0.3
3,215,303 - +1.2
3,215,354 - BWI76_RS15350 0.20 -1.7
3,216,042 + BWI76_RS15355 0.30 -0.3
3,216,052 + BWI76_RS15355 0.31 +0.6

Or see this region's nucleotide sequence