Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12955 and BWI76_RS12960 are separated by 65 nucleotides BWI76_RS12960 and BWI76_RS12965 are separated by 239 nucleotides BWI76_RS12965 and BWI76_RS12970 are separated by 111 nucleotides
BWI76_RS12955: BWI76_RS12955 - multidrug efflux RND transporter permease subunit OqxB, at 2,698,844 to 2,701,996
_RS12955
BWI76_RS12960: BWI76_RS12960 - transcriptional regulator, at 2,702,062 to 2,702,541
_RS12960
BWI76_RS12965: BWI76_RS12965 - LysR family transcriptional regulator, at 2,702,781 to 2,703,650
_RS12965
BWI76_RS12970: BWI76_RS12970 - MFS transporter, at 2,703,762 to 2,705,198
_RS12970
Position (kb)
2702
2703
2704 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2701.853 kb on - strand at 2701.935 kb on + strand at 2702.072 kb on + strand at 2702.080 kb on - strand at 2702.497 kb on + strand at 2702.575 kb on + strand at 2702.583 kb on + strand at 2702.583 kb on + strand at 2702.590 kb on + strand at 2702.591 kb on - strand at 2702.779 kb on + strand at 2702.779 kb on + strand at 2703.053 kb on - strand, within BWI76_RS12965 at 2703.123 kb on + strand, within BWI76_RS12965 at 2703.151 kb on + strand, within BWI76_RS12965 at 2703.169 kb on + strand, within BWI76_RS12965 at 2703.194 kb on - strand, within BWI76_RS12965 at 2703.338 kb on - strand, within BWI76_RS12965 at 2703.491 kb on - strand, within BWI76_RS12965 at 2703.757 kb on + strand at 2703.937 kb on + strand, within BWI76_RS12970 at 2704.208 kb on - strand, within BWI76_RS12970 at 2704.340 kb on + strand, within BWI76_RS12970 at 2704.441 kb on + strand, within BWI76_RS12970 at 2704.556 kb on + strand, within BWI76_RS12970 at 2704.587 kb on - strand, within BWI76_RS12970 at 2704.587 kb on - strand, within BWI76_RS12970 at 2704.593 kb on - strand, within BWI76_RS12970 at 2704.597 kb on - strand, within BWI76_RS12970
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,701,853 - -0.2 2,701,935 + +0.0 2,702,072 + -2.7 2,702,080 - +0.8 2,702,497 + +2.2 2,702,575 + -0.3 2,702,583 + +2.1 2,702,583 + +0.2 2,702,590 + +1.4 2,702,591 - -1.0 2,702,779 + -0.1 2,702,779 + -1.2 2,703,053 - BWI76_RS12965 0.31 -0.2 2,703,123 + BWI76_RS12965 0.39 +1.0 2,703,151 + BWI76_RS12965 0.43 +1.4 2,703,169 + BWI76_RS12965 0.45 +0.8 2,703,194 - BWI76_RS12965 0.47 +0.7 2,703,338 - BWI76_RS12965 0.64 +1.6 2,703,491 - BWI76_RS12965 0.82 +0.6 2,703,757 + +0.4 2,703,937 + BWI76_RS12970 0.12 +0.1 2,704,208 - BWI76_RS12970 0.31 +0.4 2,704,340 + BWI76_RS12970 0.40 +0.3 2,704,441 + BWI76_RS12970 0.47 -0.8 2,704,556 + BWI76_RS12970 0.55 -0.3 2,704,587 - BWI76_RS12970 0.57 +0.1 2,704,587 - BWI76_RS12970 0.57 +0.8 2,704,593 - BWI76_RS12970 0.58 -1.8 2,704,597 - BWI76_RS12970 0.58 -2.8
Or see this region's nucleotide sequence