Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11580 and BWI76_RS11585 are separated by 94 nucleotides BWI76_RS11585 and BWI76_RS11590 are separated by 166 nucleotides BWI76_RS11590 and BWI76_RS11595 are separated by 164 nucleotides
BWI76_RS11580: BWI76_RS11580 - L-asparaginase 1, at 2,436,157 to 2,437,173
_RS11580
BWI76_RS11585: BWI76_RS11585 - signal peptide peptidase A, at 2,437,268 to 2,439,121
_RS11585
BWI76_RS11590: BWI76_RS11590 - NAD(P)H nitroreductase, at 2,439,288 to 2,439,839
_RS11590
BWI76_RS11595: BWI76_RS11595 - acid phosphatase, at 2,440,004 to 2,440,750
_RS11595
Position (kb)
2437
2438
2439
2440 Strain fitness (log2 ratio)
-2
-1
0
1 at 2436.375 kb on + strand, within BWI76_RS11580 at 2436.931 kb on - strand, within BWI76_RS11580 at 2437.185 kb on + strand at 2437.206 kb on + strand at 2437.206 kb on + strand at 2437.206 kb on + strand at 2437.209 kb on - strand at 2437.214 kb on - strand at 2438.247 kb on + strand, within BWI76_RS11585 at 2438.498 kb on + strand, within BWI76_RS11585 at 2438.511 kb on + strand, within BWI76_RS11585 at 2438.721 kb on + strand, within BWI76_RS11585 at 2438.829 kb on - strand, within BWI76_RS11585 at 2439.183 kb on - strand at 2439.287 kb on + strand at 2439.308 kb on + strand at 2439.338 kb on - strand at 2439.574 kb on + strand, within BWI76_RS11590
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,436,375 + BWI76_RS11580 0.21 +0.2 2,436,931 - BWI76_RS11580 0.76 -0.6 2,437,185 + -0.6 2,437,206 + +0.2 2,437,206 + +1.2 2,437,206 + +0.4 2,437,209 - -0.1 2,437,214 - -1.1 2,438,247 + BWI76_RS11585 0.53 -1.7 2,438,498 + BWI76_RS11585 0.66 -1.6 2,438,511 + BWI76_RS11585 0.67 +0.8 2,438,721 + BWI76_RS11585 0.78 -2.7 2,438,829 - BWI76_RS11585 0.84 -0.7 2,439,183 - +0.2 2,439,287 + +0.3 2,439,308 + +0.7 2,439,338 - +0.6 2,439,574 + BWI76_RS11590 0.52 +0.9
Or see this region's nucleotide sequence