Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11385 and BWI76_RS11390 are separated by 96 nucleotides BWI76_RS11390 and BWI76_RS11395 overlap by 4 nucleotides BWI76_RS11395 and BWI76_RS11400 are separated by 180 nucleotides
BWI76_RS11385: BWI76_RS11385 - TonB-dependent siderophore receptor, at 2,399,055 to 2,401,385
_RS11385
BWI76_RS11390: BWI76_RS11390 - iron dicitrate transport regulator FecR, at 2,401,482 to 2,402,429
_RS11390
BWI76_RS11395: BWI76_RS11395 - RNA polymerase sigma factor FecI, at 2,402,426 to 2,402,947
_RS11395
BWI76_RS11400: BWI76_RS11400 - SAM-dependent methyltransferase, at 2,403,128 to 2,403,280
_RS11400
Position (kb)
2401
2402
2403 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2400.482 kb on + strand, within BWI76_RS11385 at 2400.487 kb on + strand, within BWI76_RS11385 at 2400.495 kb on - strand, within BWI76_RS11385 at 2400.495 kb on - strand, within BWI76_RS11385 at 2400.500 kb on - strand, within BWI76_RS11385 at 2400.585 kb on - strand, within BWI76_RS11385 at 2401.081 kb on + strand, within BWI76_RS11385 at 2401.158 kb on + strand at 2401.248 kb on + strand at 2401.626 kb on + strand, within BWI76_RS11390 at 2401.944 kb on + strand, within BWI76_RS11390 at 2402.090 kb on - strand, within BWI76_RS11390 at 2402.309 kb on - strand, within BWI76_RS11390 at 2402.412 kb on + strand at 2402.582 kb on - strand, within BWI76_RS11395 at 2402.707 kb on - strand, within BWI76_RS11395 at 2402.819 kb on - strand, within BWI76_RS11395 at 2402.862 kb on - strand, within BWI76_RS11395 at 2402.882 kb on + strand, within BWI76_RS11395 at 2402.890 kb on - strand, within BWI76_RS11395 at 2402.925 kb on - strand at 2403.133 kb on + strand at 2403.155 kb on + strand, within BWI76_RS11400 at 2403.163 kb on - strand, within BWI76_RS11400 at 2403.165 kb on - strand, within BWI76_RS11400 at 2403.165 kb on - strand, within BWI76_RS11400 at 2403.188 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.238 kb on + strand, within BWI76_RS11400 at 2403.248 kb on + strand, within BWI76_RS11400 at 2403.350 kb on + strand at 2403.404 kb on + strand at 2403.412 kb on - strand at 2403.421 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,400,482 + BWI76_RS11385 0.61 -1.3 2,400,487 + BWI76_RS11385 0.61 -2.3 2,400,495 - BWI76_RS11385 0.62 +1.4 2,400,495 - BWI76_RS11385 0.62 +0.0 2,400,500 - BWI76_RS11385 0.62 +0.1 2,400,585 - BWI76_RS11385 0.66 +0.4 2,401,081 + BWI76_RS11385 0.87 -0.1 2,401,158 + -0.2 2,401,248 + -0.5 2,401,626 + BWI76_RS11390 0.15 -3.2 2,401,944 + BWI76_RS11390 0.49 -1.1 2,402,090 - BWI76_RS11390 0.64 -2.5 2,402,309 - BWI76_RS11390 0.87 +0.0 2,402,412 + -0.2 2,402,582 - BWI76_RS11395 0.30 -1.2 2,402,707 - BWI76_RS11395 0.54 +0.1 2,402,819 - BWI76_RS11395 0.75 -0.5 2,402,862 - BWI76_RS11395 0.84 +1.4 2,402,882 + BWI76_RS11395 0.87 -0.5 2,402,890 - BWI76_RS11395 0.89 -0.4 2,402,925 - -0.8 2,403,133 + +0.3 2,403,155 + BWI76_RS11400 0.18 +0.6 2,403,163 - BWI76_RS11400 0.23 -0.9 2,403,165 - BWI76_RS11400 0.24 +0.2 2,403,165 - BWI76_RS11400 0.24 -1.9 2,403,188 + BWI76_RS11400 0.39 +0.9 2,403,238 + BWI76_RS11400 0.72 -0.5 2,403,238 + BWI76_RS11400 0.72 +1.1 2,403,238 + BWI76_RS11400 0.72 -1.2 2,403,238 + BWI76_RS11400 0.72 +0.2 2,403,248 + BWI76_RS11400 0.78 -0.3 2,403,350 + -0.5 2,403,404 + -0.5 2,403,412 - +1.1 2,403,421 + +0.4
Or see this region's nucleotide sequence