Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11100 and BWI76_RS11105 are separated by 143 nucleotides BWI76_RS11105 and BWI76_RS11110 are separated by 2 nucleotides BWI76_RS11110 and BWI76_RS11115 overlap by 11 nucleotides BWI76_RS11115 and BWI76_RS11120 overlap by 4 nucleotides
BWI76_RS11100: BWI76_RS11100 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 2,340,607 to 2,341,848
_RS11100
BWI76_RS11105: BWI76_RS11105 - aminodeoxychorismate lyase, at 2,341,992 to 2,342,801
_RS11105
BWI76_RS11110: BWI76_RS11110 - cell division protein YceG, at 2,342,804 to 2,343,826
_RS11110
BWI76_RS11115: BWI76_RS11115 - dTMP kinase, at 2,343,816 to 2,344,457
_RS11115
BWI76_RS11120: BWI76_RS11120 - DNA polymerase III subunit delta', at 2,344,454 to 2,345,458
_RS11120
Position (kb)
2342
2343
2344 Strain fitness (log2 ratio)
-1
0
1 at 2341.842 kb on + strand at 2342.422 kb on - strand, within BWI76_RS11105 at 2342.551 kb on + strand, within BWI76_RS11105 at 2342.551 kb on + strand, within BWI76_RS11105 at 2343.218 kb on + strand, within BWI76_RS11110 at 2343.252 kb on - strand, within BWI76_RS11110
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,341,842 + +0.3 2,342,422 - BWI76_RS11105 0.53 -1.7 2,342,551 + BWI76_RS11105 0.69 -1.7 2,342,551 + BWI76_RS11105 0.69 +1.1 2,343,218 + BWI76_RS11110 0.40 -0.8 2,343,252 - BWI76_RS11110 0.44 -1.4
Or see this region's nucleotide sequence