Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08205 and BWI76_RS08210 are separated by 8 nucleotides BWI76_RS08210 and BWI76_RS08215 are separated by 60 nucleotides
BWI76_RS08205: BWI76_RS08205 - DNA-binding transcriptional dual regulator NagC, at 1,735,483 to 1,736,703
_RS08205
BWI76_RS08210: BWI76_RS08210 - N-acetylglucosamine-6-phosphate deacetylase, at 1,736,712 to 1,737,860
_RS08210
BWI76_RS08215: BWI76_RS08215 - glucosamine-6-phosphate deaminase, at 1,737,921 to 1,738,721
_RS08215
Position (kb)
1736
1737
1738 Strain fitness (log2 ratio)
-2
-1
0
1 at 1735.730 kb on + strand, within BWI76_RS08205 at 1735.915 kb on + strand, within BWI76_RS08205 at 1736.060 kb on - strand, within BWI76_RS08205 at 1736.300 kb on - strand, within BWI76_RS08205 at 1736.394 kb on - strand, within BWI76_RS08205 at 1736.430 kb on - strand, within BWI76_RS08205 at 1737.060 kb on - strand, within BWI76_RS08210 at 1737.418 kb on - strand, within BWI76_RS08210 at 1738.242 kb on + strand, within BWI76_RS08215 at 1738.483 kb on + strand, within BWI76_RS08215 at 1738.505 kb on + strand, within BWI76_RS08215 at 1738.534 kb on + strand, within BWI76_RS08215 at 1738.696 kb on + strand at 1738.773 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 1,735,730 + BWI76_RS08205 0.20 -1.6 1,735,915 + BWI76_RS08205 0.35 -0.8 1,736,060 - BWI76_RS08205 0.47 -0.0 1,736,300 - BWI76_RS08205 0.67 +0.5 1,736,394 - BWI76_RS08205 0.75 -0.3 1,736,430 - BWI76_RS08205 0.78 +0.2 1,737,060 - BWI76_RS08210 0.30 -1.9 1,737,418 - BWI76_RS08210 0.61 -1.9 1,738,242 + BWI76_RS08215 0.40 -2.7 1,738,483 + BWI76_RS08215 0.70 +0.9 1,738,505 + BWI76_RS08215 0.73 -0.0 1,738,534 + BWI76_RS08215 0.77 +0.2 1,738,696 + +0.8 1,738,773 + +0.4
Or see this region's nucleotide sequence