Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01120 and BWI76_RS01125 overlap by 4 nucleotides BWI76_RS01125 and BWI76_RS01130 overlap by 4 nucleotides
BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826
_RS01120
BWI76_RS01125: BWI76_RS01125 - 4-alpha-L-fucosyltransferase, at 231,823 to 232,902
_RS01125
BWI76_RS01130: BWI76_RS01130 - enterobacterial common antigen polymerase, at 232,899 to 234,245
_RS01130
Position (kb) 231
232
233 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 230.926 kb on - strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 231.208 kb on + strand, within BWI76_RS01120 at 231.388 kb on + strand, within BWI76_RS01120 at 231.410 kb on + strand, within BWI76_RS01120 at 231.627 kb on - strand, within BWI76_RS01120 at 231.640 kb on + strand, within BWI76_RS01120 at 231.739 kb on - strand at 231.759 kb on - strand at 231.759 kb on - strand at 231.787 kb on + strand at 231.795 kb on - strand at 231.824 kb on + strand at 231.832 kb on - strand at 231.877 kb on + strand at 231.898 kb on + strand at 232.139 kb on - strand, within BWI76_RS01125 at 232.219 kb on - strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.542 kb on - strand, within BWI76_RS01125 at 232.712 kb on - strand, within BWI76_RS01125 at 232.727 kb on + strand, within BWI76_RS01125 at 232.768 kb on + strand, within BWI76_RS01125 at 232.795 kb on - strand at 232.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 230,926 - BWI76_RS01120 0.28 -0.9 230,953 + BWI76_RS01120 0.30 +0.3 230,953 + BWI76_RS01120 0.30 -0.7 231,208 + BWI76_RS01120 0.51 -0.5 231,388 + BWI76_RS01120 0.65 +0.5 231,410 + BWI76_RS01120 0.67 -2.5 231,627 - BWI76_RS01120 0.84 -1.9 231,640 + BWI76_RS01120 0.85 -1.6 231,739 - -1.8 231,759 - -2.3 231,759 - -0.5 231,787 + +1.0 231,795 - -0.6 231,824 + +0.3 231,832 - -2.6 231,877 + -1.3 231,898 + -3.1 232,139 - BWI76_RS01125 0.29 -0.6 232,219 - BWI76_RS01125 0.37 -1.5 232,534 + BWI76_RS01125 0.66 -3.6 232,534 + BWI76_RS01125 0.66 -1.3 232,534 + BWI76_RS01125 0.66 -0.6 232,542 - BWI76_RS01125 0.67 -1.5 232,712 - BWI76_RS01125 0.82 -1.3 232,727 + BWI76_RS01125 0.84 -2.6 232,768 + BWI76_RS01125 0.88 -1.6 232,795 - +2.0 232,839 - -0.8
Or see this region's nucleotide sequence