Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00720 and BWI76_RS00725 are separated by 149 nucleotides BWI76_RS00725 and BWI76_RS00730 are separated by 149 nucleotides BWI76_RS00730 and BWI76_RS00735 are separated by 202 nucleotides
BWI76_RS00720: BWI76_RS00720 - DNA-binding response regulator, at 146,899 to 147,597
_RS00720
BWI76_RS00725: BWI76_RS00725 - periplasmic repressor CpxP, at 147,747 to 148,250
_RS00725
BWI76_RS00730: BWI76_RS00730 - cation-efflux pump FieF, at 148,400 to 149,296
_RS00730
BWI76_RS00735: BWI76_RS00735 - ATP-dependent 6-phosphofructokinase, at 149,499 to 150,461
_RS00735
Position (kb)
148
149
150 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 147.524 kb on - strand, within BWI76_RS00720 at 147.661 kb on + strand at 147.669 kb on - strand at 147.669 kb on - strand at 147.861 kb on - strand, within BWI76_RS00725 at 147.897 kb on - strand, within BWI76_RS00725 at 148.215 kb on + strand at 148.368 kb on + strand at 148.411 kb on - strand at 148.416 kb on - strand at 148.431 kb on - strand at 148.453 kb on + strand at 148.500 kb on - strand, within BWI76_RS00730 at 148.503 kb on - strand, within BWI76_RS00730 at 148.575 kb on - strand, within BWI76_RS00730 at 148.610 kb on - strand, within BWI76_RS00730 at 148.742 kb on + strand, within BWI76_RS00730 at 148.743 kb on + strand, within BWI76_RS00730 at 148.774 kb on + strand, within BWI76_RS00730 at 148.782 kb on - strand, within BWI76_RS00730 at 148.942 kb on - strand, within BWI76_RS00730 at 148.992 kb on - strand, within BWI76_RS00730 at 149.029 kb on + strand, within BWI76_RS00730 at 149.436 kb on + strand at 149.436 kb on + strand at 149.436 kb on + strand at 149.436 kb on + strand at 149.436 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 147,524 - BWI76_RS00720 0.89 -1.0 147,661 + +1.3 147,669 - -2.0 147,669 - -2.8 147,861 - BWI76_RS00725 0.23 -1.0 147,897 - BWI76_RS00725 0.30 +0.2 148,215 + -3.0 148,368 + +1.8 148,411 - -0.0 148,416 - -1.0 148,431 - -2.1 148,453 + +0.4 148,500 - BWI76_RS00730 0.11 -1.0 148,503 - BWI76_RS00730 0.11 +0.3 148,575 - BWI76_RS00730 0.20 -0.6 148,610 - BWI76_RS00730 0.23 -2.6 148,742 + BWI76_RS00730 0.38 -0.6 148,743 + BWI76_RS00730 0.38 -0.1 148,774 + BWI76_RS00730 0.42 -0.0 148,782 - BWI76_RS00730 0.43 -1.0 148,942 - BWI76_RS00730 0.60 -0.8 148,992 - BWI76_RS00730 0.66 +0.1 149,029 + BWI76_RS00730 0.70 -1.1 149,436 + -0.2 149,436 + +0.2 149,436 + +0.1 149,436 + +1.8 149,436 + -0.0
Or see this region's nucleotide sequence