Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27305 and BWI76_RS27310 are separated by 29 nucleotides BWI76_RS27310 and BWI76_RS27315 are separated by 92 nucleotides
BWI76_RS27305: BWI76_RS27305 - glycosyl transferase, at 5,667,365 to 5,668,459
_RS27305
BWI76_RS27310: BWI76_RS27310 - glycosyltransferase, at 5,668,489 to 5,669,478
_RS27310
BWI76_RS27315: BWI76_RS27315 - 3-deoxy-D-manno-octulosonic acid transferase, at 5,669,571 to 5,670,845
_RS27315
Position (kb)
5668
5669
5670 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5667.523 kb on + strand, within BWI76_RS27305 at 5667.642 kb on + strand, within BWI76_RS27305 at 5667.840 kb on + strand, within BWI76_RS27305 at 5667.848 kb on - strand, within BWI76_RS27305 at 5667.963 kb on + strand, within BWI76_RS27305 at 5668.026 kb on - strand, within BWI76_RS27305 at 5668.074 kb on - strand, within BWI76_RS27305 at 5668.110 kb on + strand, within BWI76_RS27305 at 5668.111 kb on + strand, within BWI76_RS27305 at 5668.135 kb on + strand, within BWI76_RS27305 at 5668.143 kb on - strand, within BWI76_RS27305 at 5668.176 kb on - strand, within BWI76_RS27305 at 5668.176 kb on - strand, within BWI76_RS27305 at 5668.325 kb on + strand, within BWI76_RS27305 at 5668.345 kb on + strand, within BWI76_RS27305 at 5668.362 kb on + strand at 5668.416 kb on - strand at 5668.445 kb on - strand at 5668.589 kb on + strand, within BWI76_RS27310 at 5668.631 kb on + strand, within BWI76_RS27310 at 5668.746 kb on + strand, within BWI76_RS27310 at 5668.754 kb on - strand, within BWI76_RS27310 at 5668.776 kb on + strand, within BWI76_RS27310 at 5668.794 kb on - strand, within BWI76_RS27310 at 5668.948 kb on - strand, within BWI76_RS27310 at 5669.038 kb on - strand, within BWI76_RS27310 at 5669.103 kb on + strand, within BWI76_RS27310 at 5669.118 kb on + strand at 5669.118 kb on + strand, within BWI76_RS27310 at 5669.126 kb on - strand, within BWI76_RS27310 at 5669.137 kb on - strand, within BWI76_RS27310 at 5669.228 kb on + strand, within BWI76_RS27310 at 5669.325 kb on + strand, within BWI76_RS27310 at 5669.473 kb on + strand at 5669.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,667,523 + BWI76_RS27305 0.14 -1.5 5,667,642 + BWI76_RS27305 0.25 -3.4 5,667,840 + BWI76_RS27305 0.43 -0.6 5,667,848 - BWI76_RS27305 0.44 -1.2 5,667,963 + BWI76_RS27305 0.55 -2.7 5,668,026 - BWI76_RS27305 0.60 -1.0 5,668,074 - BWI76_RS27305 0.65 -2.1 5,668,110 + BWI76_RS27305 0.68 -1.1 5,668,111 + BWI76_RS27305 0.68 -1.0 5,668,135 + BWI76_RS27305 0.70 -1.8 5,668,143 - BWI76_RS27305 0.71 -1.6 5,668,176 - BWI76_RS27305 0.74 -1.1 5,668,176 - BWI76_RS27305 0.74 -0.7 5,668,325 + BWI76_RS27305 0.88 -2.8 5,668,345 + BWI76_RS27305 0.89 -1.0 5,668,362 + -1.0 5,668,416 - +1.0 5,668,445 - -1.8 5,668,589 + BWI76_RS27310 0.10 -1.5 5,668,631 + BWI76_RS27310 0.14 -2.3 5,668,746 + BWI76_RS27310 0.26 -2.1 5,668,754 - BWI76_RS27310 0.27 -2.6 5,668,776 + BWI76_RS27310 0.29 -1.9 5,668,794 - BWI76_RS27310 0.31 -2.0 5,668,948 - BWI76_RS27310 0.46 -1.9 5,669,038 - BWI76_RS27310 0.55 -1.8 5,669,103 + BWI76_RS27310 0.62 -2.0 5,669,118 + +0.7 5,669,118 + BWI76_RS27310 0.64 -1.2 5,669,126 - BWI76_RS27310 0.64 -3.3 5,669,137 - BWI76_RS27310 0.65 -1.6 5,669,228 + BWI76_RS27310 0.75 -1.9 5,669,325 + BWI76_RS27310 0.84 -1.7 5,669,473 + +0.2 5,669,506 - +0.6
Or see this region's nucleotide sequence