Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24285 and BWI76_RS24290 are separated by 132 nucleotides BWI76_RS24290 and BWI76_RS24295 are separated by 31 nucleotides
BWI76_RS24285: BWI76_RS24285 - cystathionine beta-lyase, at 5,059,322 to 5,060,509
_RS24285
BWI76_RS24290: BWI76_RS24290 - membrane protein, at 5,060,642 to 5,061,301
_RS24290
BWI76_RS24295: BWI76_RS24295 - AraC family transcriptional regulator, at 5,061,333 to 5,062,232
_RS24295
Position (kb)
5060
5061
5062 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5059.658 kb on - strand, within BWI76_RS24285 at 5059.746 kb on + strand, within BWI76_RS24285 at 5059.750 kb on + strand, within BWI76_RS24285 at 5059.900 kb on + strand, within BWI76_RS24285 at 5060.087 kb on - strand, within BWI76_RS24285 at 5060.098 kb on + strand, within BWI76_RS24285 at 5060.102 kb on - strand, within BWI76_RS24285 at 5060.119 kb on - strand, within BWI76_RS24285 at 5060.286 kb on - strand, within BWI76_RS24285 at 5060.592 kb on + strand at 5060.721 kb on + strand, within BWI76_RS24290 at 5060.906 kb on - strand, within BWI76_RS24290 at 5060.949 kb on - strand, within BWI76_RS24290 at 5061.063 kb on + strand, within BWI76_RS24290 at 5061.202 kb on + strand, within BWI76_RS24290 at 5061.347 kb on - strand at 5061.432 kb on - strand, within BWI76_RS24295 at 5061.572 kb on + strand, within BWI76_RS24295 at 5061.662 kb on - strand at 5061.719 kb on + strand, within BWI76_RS24295 at 5061.953 kb on + strand, within BWI76_RS24295 at 5062.013 kb on + strand, within BWI76_RS24295 at 5062.126 kb on - strand, within BWI76_RS24295 at 5062.149 kb on - strand at 5062.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,059,658 - BWI76_RS24285 0.28 -4.8 5,059,746 + BWI76_RS24285 0.36 -3.9 5,059,750 + BWI76_RS24285 0.36 -1.3 5,059,900 + BWI76_RS24285 0.49 -3.2 5,060,087 - BWI76_RS24285 0.64 -2.3 5,060,098 + BWI76_RS24285 0.65 -3.7 5,060,102 - BWI76_RS24285 0.66 -2.7 5,060,119 - BWI76_RS24285 0.67 -3.7 5,060,286 - BWI76_RS24285 0.81 -2.9 5,060,592 + -0.6 5,060,721 + BWI76_RS24290 0.12 -2.4 5,060,906 - BWI76_RS24290 0.40 -1.0 5,060,949 - BWI76_RS24290 0.47 -0.8 5,061,063 + BWI76_RS24290 0.64 -2.1 5,061,202 + BWI76_RS24290 0.85 -3.1 5,061,347 - -0.1 5,061,432 - BWI76_RS24295 0.11 +0.1 5,061,572 + BWI76_RS24295 0.27 +0.7 5,061,662 - -0.6 5,061,719 + BWI76_RS24295 0.43 +2.1 5,061,953 + BWI76_RS24295 0.69 +0.3 5,062,013 + BWI76_RS24295 0.76 -0.0 5,062,126 - BWI76_RS24295 0.88 +1.0 5,062,149 - -0.0 5,062,252 - +1.0
Or see this region's nucleotide sequence