Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24025 and BWI76_RS24030 are separated by 211 nucleotides BWI76_RS24030 and BWI76_RS24035 are separated by 8 nucleotides BWI76_RS24035 and BWI76_RS24040 are separated by 118 nucleotides BWI76_RS24040 and BWI76_RS24045 are separated by 15 nucleotides BWI76_RS24045 and BWI76_RS24050 are separated by 62 nucleotides
BWI76_RS24025: BWI76_RS24025 - agmatinase, at 5,014,228 to 5,015,148
_RS24025
BWI76_RS24030: BWI76_RS24030 - arginine decarboxylase, at 5,015,360 to 5,017,336
_RS24030
BWI76_RS24035: BWI76_RS24035 - hypothetical protein, at 5,017,345 to 5,017,491
_RS24035
BWI76_RS24040: BWI76_RS24040 - hypothetical protein, at 5,017,610 to 5,017,855
_RS24040
BWI76_RS24045: BWI76_RS24045 - hypothetical protein, at 5,017,871 to 5,018,116
_RS24045
BWI76_RS24050: BWI76_RS24050 - methionine adenosyltransferase, at 5,018,179 to 5,019,333
_RS24050
Position (kb) 5015
5016
5017
5018 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5014.443 kb on + strand, within BWI76_RS24025 at 5014.495 kb on + strand, within BWI76_RS24025 at 5014.605 kb on - strand, within BWI76_RS24025 at 5014.643 kb on + strand, within BWI76_RS24025 at 5014.643 kb on + strand, within BWI76_RS24025 at 5014.643 kb on + strand, within BWI76_RS24025 at 5014.651 kb on - strand, within BWI76_RS24025 at 5014.891 kb on - strand, within BWI76_RS24025 at 5015.150 kb on + strand at 5015.644 kb on + strand, within BWI76_RS24030 at 5016.002 kb on - strand, within BWI76_RS24030 at 5016.088 kb on - strand, within BWI76_RS24030 at 5016.255 kb on + strand, within BWI76_RS24030 at 5016.289 kb on + strand, within BWI76_RS24030 at 5016.451 kb on + strand, within BWI76_RS24030 at 5016.543 kb on - strand, within BWI76_RS24030 at 5016.881 kb on - strand, within BWI76_RS24030 at 5016.923 kb on - strand, within BWI76_RS24030 at 5016.949 kb on + strand, within BWI76_RS24030 at 5017.062 kb on + strand, within BWI76_RS24030 at 5017.096 kb on - strand, within BWI76_RS24030 at 5017.314 kb on + strand at 5017.322 kb on - strand at 5017.408 kb on + strand, within BWI76_RS24035 at 5017.408 kb on + strand, within BWI76_RS24035 at 5017.416 kb on - strand, within BWI76_RS24035 at 5017.433 kb on + strand, within BWI76_RS24035 at 5017.446 kb on - strand, within BWI76_RS24035 at 5017.497 kb on - strand at 5017.503 kb on + strand at 5017.503 kb on + strand at 5017.511 kb on - strand at 5017.511 kb on - strand at 5017.511 kb on - strand at 5017.511 kb on - strand at 5017.563 kb on - strand at 5017.592 kb on + strand at 5017.602 kb on - strand at 5017.620 kb on - strand at 5017.620 kb on - strand at 5017.629 kb on + strand at 5017.651 kb on - strand, within BWI76_RS24040 at 5017.724 kb on + strand, within BWI76_RS24040 at 5017.756 kb on + strand, within BWI76_RS24040 at 5017.829 kb on - strand, within BWI76_RS24040 at 5017.868 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 5,014,443 + BWI76_RS24025 0.23 -2.6 5,014,495 + BWI76_RS24025 0.29 -1.7 5,014,605 - BWI76_RS24025 0.41 -0.4 5,014,643 + BWI76_RS24025 0.45 -1.6 5,014,643 + BWI76_RS24025 0.45 -2.0 5,014,643 + BWI76_RS24025 0.45 -0.5 5,014,651 - BWI76_RS24025 0.46 -0.2 5,014,891 - BWI76_RS24025 0.72 -0.4 5,015,150 + +1.2 5,015,644 + BWI76_RS24030 0.14 -2.0 5,016,002 - BWI76_RS24030 0.32 -0.0 5,016,088 - BWI76_RS24030 0.37 +0.7 5,016,255 + BWI76_RS24030 0.45 -0.1 5,016,289 + BWI76_RS24030 0.47 -0.2 5,016,451 + BWI76_RS24030 0.55 -1.9 5,016,543 - BWI76_RS24030 0.60 -0.8 5,016,881 - BWI76_RS24030 0.77 -0.6 5,016,923 - BWI76_RS24030 0.79 -0.7 5,016,949 + BWI76_RS24030 0.80 +1.1 5,017,062 + BWI76_RS24030 0.86 -0.8 5,017,096 - BWI76_RS24030 0.88 -0.1 5,017,314 + +0.9 5,017,322 - +0.2 5,017,408 + BWI76_RS24035 0.43 -1.6 5,017,408 + BWI76_RS24035 0.43 -0.1 5,017,416 - BWI76_RS24035 0.48 +1.8 5,017,433 + BWI76_RS24035 0.60 -1.9 5,017,446 - BWI76_RS24035 0.69 +2.3 5,017,497 - -1.0 5,017,503 + -1.6 5,017,503 + +1.0 5,017,511 - -0.0 5,017,511 - +0.3 5,017,511 - +1.5 5,017,511 - +0.1 5,017,563 - +2.0 5,017,592 + +0.0 5,017,602 - -0.5 5,017,620 - -0.2 5,017,620 - -0.5 5,017,629 + +0.7 5,017,651 - BWI76_RS24040 0.17 +1.0 5,017,724 + BWI76_RS24040 0.46 +0.3 5,017,756 + BWI76_RS24040 0.59 +0.3 5,017,829 - BWI76_RS24040 0.89 +1.7 5,017,868 + -0.6
Or see this region's nucleotide sequence