Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20225 and BWI76_RS20230 are separated by 86 nucleotides BWI76_RS20230 and BWI76_RS20235 are separated by 336 nucleotides BWI76_RS20235 and BWI76_RS20240 are separated by 76 nucleotides
BWI76_RS20225: BWI76_RS20225 - hypothetical protein, at 4,239,004 to 4,239,498
_RS20225
BWI76_RS20230: BWI76_RS20230 - hypothetical protein, at 4,239,585 to 4,240,040
_RS20230
BWI76_RS20235: BWI76_RS20235 - acetate kinase, at 4,240,377 to 4,241,579
_RS20235
BWI76_RS20240: BWI76_RS20240 - phosphate acetyltransferase, at 4,241,656 to 4,243,800
_RS20240
Position (kb)
4240
4241
4242 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4239.469 kb on + strand at 4239.516 kb on + strand at 4239.637 kb on - strand, within BWI76_RS20230 at 4239.643 kb on + strand, within BWI76_RS20230 at 4240.035 kb on + strand at 4240.164 kb on - strand at 4240.747 kb on + strand, within BWI76_RS20235 at 4241.607 kb on + strand at 4241.964 kb on - strand, within BWI76_RS20240
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 4,239,469 + +0.9 4,239,516 + -0.5 4,239,637 - BWI76_RS20230 0.11 -1.1 4,239,643 + BWI76_RS20230 0.13 -3.6 4,240,035 + -2.9 4,240,164 - -1.3 4,240,747 + BWI76_RS20235 0.31 +0.1 4,241,607 + -0.3 4,241,964 - BWI76_RS20240 0.14 -1.5
Or see this region's nucleotide sequence