Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17970 and BWI76_RS17975 are separated by 236 nucleotides BWI76_RS17975 and BWI76_RS17980 are separated by 19 nucleotides BWI76_RS17980 and BWI76_RS17985 are separated by 97 nucleotides
BWI76_RS17970: BWI76_RS17970 - zinc metalloprotease HtpX, at 3,732,923 to 3,733,804
_RS17970
BWI76_RS17975: BWI76_RS17975 - tail-specific protease, at 3,734,041 to 3,736,089
_RS17975
BWI76_RS17980: BWI76_RS17980 - RNA chaperone ProQ, at 3,736,109 to 3,736,792
_RS17980
BWI76_RS17985: BWI76_RS17985 - hypothetical protein, at 3,736,890 to 3,737,387
_RS17985
Position (kb)
3734
3735
3736
3737 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3733.496 kb on + strand, within BWI76_RS17970 at 3734.532 kb on + strand, within BWI76_RS17975 at 3734.577 kb on + strand, within BWI76_RS17975 at 3734.611 kb on - strand, within BWI76_RS17975 at 3734.890 kb on - strand, within BWI76_RS17975 at 3735.093 kb on + strand, within BWI76_RS17975 at 3735.745 kb on - strand, within BWI76_RS17975 at 3735.988 kb on - strand at 3736.159 kb on + strand at 3736.446 kb on + strand, within BWI76_RS17980 at 3736.446 kb on + strand, within BWI76_RS17980 at 3736.454 kb on - strand, within BWI76_RS17980 at 3736.575 kb on - strand, within BWI76_RS17980 at 3736.575 kb on - strand, within BWI76_RS17980 at 3736.582 kb on + strand, within BWI76_RS17980 at 3736.871 kb on + strand at 3736.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,733,496 + BWI76_RS17970 0.65 +0.1 3,734,532 + BWI76_RS17975 0.24 -2.5 3,734,577 + BWI76_RS17975 0.26 +0.1 3,734,611 - BWI76_RS17975 0.28 -3.3 3,734,890 - BWI76_RS17975 0.41 -1.9 3,735,093 + BWI76_RS17975 0.51 -1.5 3,735,745 - BWI76_RS17975 0.83 -4.1 3,735,988 - -1.5 3,736,159 + +1.1 3,736,446 + BWI76_RS17980 0.49 -5.5 3,736,446 + BWI76_RS17980 0.49 -3.1 3,736,454 - BWI76_RS17980 0.50 -1.5 3,736,575 - BWI76_RS17980 0.68 -1.7 3,736,575 - BWI76_RS17980 0.68 -3.9 3,736,582 + BWI76_RS17980 0.69 -2.7 3,736,871 + -1.2 3,736,871 + +1.1
Or see this region's nucleotide sequence