Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17500 and BWI76_RS17505 are separated by 62 nucleotides BWI76_RS17505 and BWI76_RS17510 are separated by 63 nucleotides
BWI76_RS17500: BWI76_RS17500 - knotted carbamoyltransferase YgeW, at 3,628,648 to 3,629,835
_RS17500
BWI76_RS17505: BWI76_RS17505 - PLP-dependent lyase/thiolase, at 3,629,898 to 3,631,097
_RS17505
BWI76_RS17510: BWI76_RS17510 - selenium metabolism hydrolase, at 3,631,161 to 3,632,372
_RS17510
Position (kb)
3629
3630
3631
3632 Strain fitness (log2 ratio)
-2
-1
0
1 at 3629.083 kb on - strand, within BWI76_RS17500 at 3629.302 kb on + strand, within BWI76_RS17500 at 3629.330 kb on + strand, within BWI76_RS17500 at 3629.350 kb on - strand, within BWI76_RS17500 at 3629.579 kb on + strand, within BWI76_RS17500 at 3629.813 kb on - strand at 3629.858 kb on + strand at 3629.868 kb on - strand at 3629.868 kb on - strand at 3630.207 kb on - strand, within BWI76_RS17505 at 3630.556 kb on - strand, within BWI76_RS17505 at 3630.686 kb on + strand, within BWI76_RS17505 at 3630.694 kb on + strand, within BWI76_RS17505 at 3630.739 kb on - strand, within BWI76_RS17505 at 3630.775 kb on - strand, within BWI76_RS17505 at 3630.894 kb on - strand, within BWI76_RS17505 at 3630.894 kb on - strand, within BWI76_RS17505 at 3631.040 kb on - strand at 3631.042 kb on + strand at 3631.146 kb on - strand at 3631.498 kb on + strand, within BWI76_RS17510 at 3631.631 kb on + strand, within BWI76_RS17510 at 3632.060 kb on + strand, within BWI76_RS17510 at 3632.070 kb on + strand, within BWI76_RS17510 at 3632.070 kb on + strand, within BWI76_RS17510 at 3632.078 kb on - strand, within BWI76_RS17510
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,629,083 - BWI76_RS17500 0.37 -0.5 3,629,302 + BWI76_RS17500 0.55 -1.3 3,629,330 + BWI76_RS17500 0.57 -0.8 3,629,350 - BWI76_RS17500 0.59 +0.5 3,629,579 + BWI76_RS17500 0.78 -0.1 3,629,813 - -1.7 3,629,858 + +0.6 3,629,868 - -1.4 3,629,868 - -0.1 3,630,207 - BWI76_RS17505 0.26 +0.5 3,630,556 - BWI76_RS17505 0.55 -2.0 3,630,686 + BWI76_RS17505 0.66 +1.0 3,630,694 + BWI76_RS17505 0.66 -0.0 3,630,739 - BWI76_RS17505 0.70 -0.8 3,630,775 - BWI76_RS17505 0.73 -0.0 3,630,894 - BWI76_RS17505 0.83 +0.6 3,630,894 - BWI76_RS17505 0.83 -0.4 3,631,040 - -0.5 3,631,042 + +0.4 3,631,146 - +0.1 3,631,498 + BWI76_RS17510 0.28 -0.3 3,631,631 + BWI76_RS17510 0.39 +1.5 3,632,060 + BWI76_RS17510 0.74 +0.2 3,632,070 + BWI76_RS17510 0.75 -0.0 3,632,070 + BWI76_RS17510 0.75 -0.1 3,632,078 - BWI76_RS17510 0.76 +0.6
Or see this region's nucleotide sequence