Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17310 and BWI76_RS17315 are separated by 107 nucleotides BWI76_RS17315 and BWI76_RS17320 overlap by 4 nucleotides BWI76_RS17320 and BWI76_RS17325 overlap by 1 nucleotides BWI76_RS17325 and BWI76_RS17330 overlap by 4 nucleotides
BWI76_RS17310: BWI76_RS17310 - AsnC family transcriptional regulator, at 3,583,949 to 3,584,392
_RS17310
BWI76_RS17315: BWI76_RS17315 - EamA family transporter, at 3,584,500 to 3,585,395
_RS17315
BWI76_RS17320: BWI76_RS17320 - respiratory nitrate reductase subunit gamma, at 3,585,392 to 3,586,069
_RS17320
BWI76_RS17325: BWI76_RS17325 - nitrate reductase molybdenum cofactor assembly chaperone, at 3,586,069 to 3,586,779
_RS17325
BWI76_RS17330: BWI76_RS17330 - nitrate reductase subunit beta, at 3,586,776 to 3,588,311
_RS17330
Position (kb)
3585
3586
3587 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3584.479 kb on - strand at 3584.626 kb on - strand, within BWI76_RS17315 at 3584.697 kb on + strand, within BWI76_RS17315 at 3584.793 kb on + strand, within BWI76_RS17315 at 3584.798 kb on + strand, within BWI76_RS17315 at 3584.801 kb on - strand, within BWI76_RS17315 at 3584.807 kb on - strand, within BWI76_RS17315 at 3585.328 kb on + strand at 3585.634 kb on + strand, within BWI76_RS17320 at 3585.852 kb on + strand, within BWI76_RS17320 at 3585.976 kb on - strand, within BWI76_RS17320 at 3586.413 kb on + strand, within BWI76_RS17325 at 3586.423 kb on + strand, within BWI76_RS17325 at 3586.513 kb on - strand, within BWI76_RS17325 at 3586.573 kb on + strand, within BWI76_RS17325 at 3586.661 kb on - strand, within BWI76_RS17325 at 3587.002 kb on + strand, within BWI76_RS17330
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,584,479 - -0.5 3,584,626 - BWI76_RS17315 0.14 -1.4 3,584,697 + BWI76_RS17315 0.22 -2.6 3,584,793 + BWI76_RS17315 0.33 -0.1 3,584,798 + BWI76_RS17315 0.33 +1.7 3,584,801 - BWI76_RS17315 0.34 -0.5 3,584,807 - BWI76_RS17315 0.34 -2.1 3,585,328 + +0.4 3,585,634 + BWI76_RS17320 0.36 +1.3 3,585,852 + BWI76_RS17320 0.68 +2.7 3,585,976 - BWI76_RS17320 0.86 -0.1 3,586,413 + BWI76_RS17325 0.48 -0.9 3,586,423 + BWI76_RS17325 0.50 -1.0 3,586,513 - BWI76_RS17325 0.62 +0.0 3,586,573 + BWI76_RS17325 0.71 -0.2 3,586,661 - BWI76_RS17325 0.83 +0.2 3,587,002 + BWI76_RS17330 0.15 -0.6
Or see this region's nucleotide sequence