Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14565 and BWI76_RS14570 are separated by 28 nucleotides BWI76_RS14570 and BWI76_RS14575 are separated by 88 nucleotides BWI76_RS14575 and BWI76_RS14580 overlap by 1 nucleotides
BWI76_RS14565: BWI76_RS14565 - cysteine hydrolase, at 3,058,393 to 3,058,926
_RS14565
BWI76_RS14570: BWI76_RS14570 - hypothetical protein, at 3,058,955 to 3,059,515
_RS14570
BWI76_RS14575: BWI76_RS14575 - TENA/THI-4 family protein, at 3,059,604 to 3,060,248
_RS14575
BWI76_RS14580: BWI76_RS14580 - hypothetical protein, at 3,060,248 to 3,061,093
_RS14580
Position (kb)
3058
3059
3060 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3058.017 kb on + strand at 3058.022 kb on + strand at 3058.025 kb on - strand at 3058.025 kb on - strand at 3058.034 kb on - strand at 3058.071 kb on + strand at 3058.117 kb on - strand at 3058.248 kb on - strand at 3058.257 kb on - strand at 3058.266 kb on + strand at 3058.282 kb on + strand at 3058.318 kb on + strand at 3058.353 kb on - strand at 3058.387 kb on + strand at 3058.596 kb on - strand, within BWI76_RS14565 at 3059.281 kb on + strand, within BWI76_RS14570 at 3059.324 kb on + strand, within BWI76_RS14570 at 3059.353 kb on - strand, within BWI76_RS14570 at 3059.370 kb on - strand, within BWI76_RS14570 at 3059.548 kb on + strand at 3059.548 kb on + strand at 3059.651 kb on - strand at 3059.768 kb on + strand, within BWI76_RS14575 at 3059.982 kb on - strand, within BWI76_RS14575 at 3060.294 kb on + strand at 3060.335 kb on - strand, within BWI76_RS14580 at 3060.403 kb on + strand, within BWI76_RS14580 at 3060.411 kb on - strand, within BWI76_RS14580 at 3060.477 kb on + strand, within BWI76_RS14580
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 3,058,017 + +1.1 3,058,022 + -0.7 3,058,025 - +0.7 3,058,025 - +0.9 3,058,034 - +1.1 3,058,071 + -0.3 3,058,117 - +0.3 3,058,248 - +1.3 3,058,257 - +1.2 3,058,266 + -0.6 3,058,282 + +0.6 3,058,318 + +0.2 3,058,353 - -1.8 3,058,387 + +0.6 3,058,596 - BWI76_RS14565 0.38 -2.6 3,059,281 + BWI76_RS14570 0.58 -1.1 3,059,324 + BWI76_RS14570 0.66 -0.7 3,059,353 - BWI76_RS14570 0.71 -0.2 3,059,370 - BWI76_RS14570 0.74 -0.6 3,059,548 + +0.3 3,059,548 + +0.5 3,059,651 - +1.3 3,059,768 + BWI76_RS14575 0.25 +0.8 3,059,982 - BWI76_RS14575 0.59 +0.2 3,060,294 + -1.7 3,060,335 - BWI76_RS14580 0.10 -0.5 3,060,403 + BWI76_RS14580 0.18 -3.6 3,060,411 - BWI76_RS14580 0.19 +0.2 3,060,477 + BWI76_RS14580 0.27 -0.3
Or see this region's nucleotide sequence