Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12995 and BWI76_RS13000 are separated by 265 nucleotides BWI76_RS13000 and BWI76_RS13005 are separated by 358 nucleotides
BWI76_RS12995: BWI76_RS12995 - hypothetical protein, at 2,710,404 to 2,711,372
_RS12995
BWI76_RS13000: BWI76_RS13000 - hypothetical protein, at 2,711,638 to 2,712,438
_RS13000
BWI76_RS13005: BWI76_RS13005 - thiamine pyrophosphate protein TPP-binding protein, at 2,712,797 to 2,714,491
_RS13005
Position (kb)
2711
2712
2713 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2710.740 kb on + strand, within BWI76_RS12995 at 2710.740 kb on + strand, within BWI76_RS12995 at 2710.740 kb on + strand, within BWI76_RS12995 at 2710.783 kb on - strand, within BWI76_RS12995 at 2710.790 kb on - strand, within BWI76_RS12995 at 2710.886 kb on + strand, within BWI76_RS12995 at 2710.902 kb on - strand, within BWI76_RS12995 at 2710.907 kb on - strand, within BWI76_RS12995 at 2710.940 kb on + strand, within BWI76_RS12995 at 2710.940 kb on + strand, within BWI76_RS12995 at 2710.948 kb on - strand, within BWI76_RS12995 at 2710.948 kb on - strand, within BWI76_RS12995 at 2710.948 kb on - strand, within BWI76_RS12995 at 2710.948 kb on - strand at 2710.948 kb on - strand, within BWI76_RS12995 at 2710.948 kb on - strand, within BWI76_RS12995 at 2711.018 kb on + strand, within BWI76_RS12995 at 2711.028 kb on + strand, within BWI76_RS12995 at 2711.118 kb on + strand, within BWI76_RS12995 at 2711.118 kb on + strand, within BWI76_RS12995 at 2711.126 kb on - strand, within BWI76_RS12995 at 2711.164 kb on + strand, within BWI76_RS12995 at 2711.164 kb on + strand, within BWI76_RS12995 at 2711.164 kb on + strand, within BWI76_RS12995 at 2711.172 kb on - strand, within BWI76_RS12995 at 2711.185 kb on + strand, within BWI76_RS12995 at 2711.207 kb on + strand at 2711.218 kb on + strand, within BWI76_RS12995 at 2711.235 kb on + strand, within BWI76_RS12995 at 2711.241 kb on + strand, within BWI76_RS12995 at 2711.283 kb on + strand at 2711.286 kb on + strand at 2711.295 kb on - strand at 2711.295 kb on - strand at 2711.336 kb on + strand at 2711.376 kb on + strand at 2711.401 kb on - strand at 2711.407 kb on - strand at 2711.408 kb on - strand at 2711.485 kb on - strand at 2711.499 kb on + strand at 2711.499 kb on + strand at 2711.507 kb on - strand at 2711.507 kb on - strand at 2711.526 kb on + strand at 2711.542 kb on + strand at 2711.543 kb on + strand at 2711.582 kb on + strand at 2711.601 kb on + strand at 2711.601 kb on + strand at 2711.631 kb on - strand at 2711.635 kb on + strand at 2711.664 kb on + strand at 2711.676 kb on - strand at 2711.676 kb on - strand at 2711.778 kb on - strand, within BWI76_RS13000 at 2711.794 kb on - strand, within BWI76_RS13000 at 2711.796 kb on + strand, within BWI76_RS13000 at 2711.796 kb on + strand, within BWI76_RS13000 at 2711.891 kb on - strand, within BWI76_RS13000 at 2711.898 kb on - strand, within BWI76_RS13000 at 2711.954 kb on + strand, within BWI76_RS13000 at 2712.277 kb on - strand, within BWI76_RS13000 at 2712.318 kb on - strand, within BWI76_RS13000 at 2712.346 kb on + strand, within BWI76_RS13000 at 2712.354 kb on - strand, within BWI76_RS13000 at 2712.387 kb on + strand at 2712.467 kb on + strand at 2712.467 kb on + strand at 2712.475 kb on - strand at 2712.475 kb on - strand at 2712.484 kb on - strand at 2712.557 kb on + strand at 2712.559 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.562 kb on + strand at 2712.565 kb on - strand at 2712.570 kb on - strand at 2712.570 kb on - strand at 2712.570 kb on - strand at 2712.627 kb on + strand at 2712.635 kb on - strand at 2712.640 kb on + strand at 2712.648 kb on - strand at 2712.674 kb on + strand at 2712.682 kb on - strand at 2712.687 kb on + strand at 2712.695 kb on - strand at 2712.695 kb on - strand at 2712.695 kb on - strand at 2712.695 kb on - strand at 2712.968 kb on + strand, within BWI76_RS13005 at 2713.005 kb on + strand, within BWI76_RS13005 at 2713.122 kb on + strand, within BWI76_RS13005 at 2713.168 kb on + strand, within BWI76_RS13005 at 2713.218 kb on + strand, within BWI76_RS13005 at 2713.226 kb on - strand, within BWI76_RS13005
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 2,710,740 + BWI76_RS12995 0.35 -0.2 2,710,740 + BWI76_RS12995 0.35 -0.3 2,710,740 + BWI76_RS12995 0.35 -1.2 2,710,783 - BWI76_RS12995 0.39 -0.3 2,710,790 - BWI76_RS12995 0.40 -0.7 2,710,886 + BWI76_RS12995 0.50 +0.7 2,710,902 - BWI76_RS12995 0.51 +0.5 2,710,907 - BWI76_RS12995 0.52 +0.7 2,710,940 + BWI76_RS12995 0.55 -1.5 2,710,940 + BWI76_RS12995 0.55 +0.2 2,710,948 - BWI76_RS12995 0.56 +1.7 2,710,948 - BWI76_RS12995 0.56 +0.7 2,710,948 - BWI76_RS12995 0.56 +0.6 2,710,948 - +0.3 2,710,948 - BWI76_RS12995 0.56 +0.3 2,710,948 - BWI76_RS12995 0.56 -1.3 2,711,018 + BWI76_RS12995 0.63 +0.1 2,711,028 + BWI76_RS12995 0.64 +0.8 2,711,118 + BWI76_RS12995 0.74 +1.3 2,711,118 + BWI76_RS12995 0.74 +0.1 2,711,126 - BWI76_RS12995 0.75 -0.6 2,711,164 + BWI76_RS12995 0.78 +0.5 2,711,164 + BWI76_RS12995 0.78 -1.3 2,711,164 + BWI76_RS12995 0.78 +0.3 2,711,172 - BWI76_RS12995 0.79 +0.2 2,711,185 + BWI76_RS12995 0.81 -1.3 2,711,207 + -0.3 2,711,218 + BWI76_RS12995 0.84 +0.8 2,711,235 + BWI76_RS12995 0.86 +1.2 2,711,241 + BWI76_RS12995 0.86 +0.7 2,711,283 + -0.5 2,711,286 + +0.3 2,711,295 - +0.7 2,711,295 - -0.5 2,711,336 + +0.3 2,711,376 + -0.0 2,711,401 - -1.4 2,711,407 - -0.8 2,711,408 - +1.7 2,711,485 - -3.2 2,711,499 + +0.4 2,711,499 + +1.9 2,711,507 - -2.4 2,711,507 - -1.0 2,711,526 + +2.3 2,711,542 + +0.7 2,711,543 + +0.5 2,711,582 + -2.4 2,711,601 + -0.3 2,711,601 + -0.0 2,711,631 - +1.5 2,711,635 + +0.7 2,711,664 + -2.0 2,711,676 - -1.7 2,711,676 - +0.4 2,711,778 - BWI76_RS13000 0.17 -0.3 2,711,794 - BWI76_RS13000 0.19 +0.2 2,711,796 + BWI76_RS13000 0.20 -1.0 2,711,796 + BWI76_RS13000 0.20 -0.8 2,711,891 - BWI76_RS13000 0.32 +0.0 2,711,898 - BWI76_RS13000 0.32 -0.2 2,711,954 + BWI76_RS13000 0.39 -1.2 2,712,277 - BWI76_RS13000 0.80 +0.5 2,712,318 - BWI76_RS13000 0.85 -0.3 2,712,346 + BWI76_RS13000 0.88 +0.6 2,712,354 - BWI76_RS13000 0.89 -1.7 2,712,387 + +1.2 2,712,467 + -1.6 2,712,467 + -2.0 2,712,475 - -2.2 2,712,475 - +0.4 2,712,484 - -0.2 2,712,557 + +1.3 2,712,559 + +0.3 2,712,562 + +0.3 2,712,562 + -0.1 2,712,562 + -0.0 2,712,562 + -1.1 2,712,562 + +0.2 2,712,565 - +0.6 2,712,570 - -1.5 2,712,570 - -0.3 2,712,570 - +0.6 2,712,627 + +1.4 2,712,635 - +0.9 2,712,640 + -0.4 2,712,648 - +0.6 2,712,674 + +1.0 2,712,682 - -1.0 2,712,687 + +0.1 2,712,695 - -0.7 2,712,695 - +0.1 2,712,695 - +0.0 2,712,695 - +0.5 2,712,968 + BWI76_RS13005 0.10 -0.1 2,713,005 + BWI76_RS13005 0.12 +1.1 2,713,122 + BWI76_RS13005 0.19 -0.4 2,713,168 + BWI76_RS13005 0.22 +0.2 2,713,218 + BWI76_RS13005 0.25 +0.2 2,713,226 - BWI76_RS13005 0.25 -0.3
Or see this region's nucleotide sequence