Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10050

Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotidesBWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotidesBWI76_RS10055 and BWI76_RS10060 are separated by 51 nucleotides BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525 _RS10045 BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270 _RS10050 BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074 _RS10055 BWI76_RS10060: BWI76_RS10060 - acylphosphatase, at 2,131,126 to 2,131,407 _RS10060 Position (kb) 2129 2130 2131Strain fitness (log2 ratio) -1 0 1 2at 2128.762 kb on - strand, within BWI76_RS10045at 2128.900 kb on - strand, within BWI76_RS10045at 2129.803 kb on + strand, within BWI76_RS10050at 2129.811 kb on - strand, within BWI76_RS10050at 2129.980 kb on + strand, within BWI76_RS10050at 2130.069 kb on - strand, within BWI76_RS10050at 2130.140 kb on + strand, within BWI76_RS10050at 2130.164 kb on + strand, within BWI76_RS10050at 2130.246 kb on + strandat 2130.784 kb on + strand, within BWI76_RS10055at 2130.866 kb on + strand, within BWI76_RS10055at 2131.088 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
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2,128,762 - BWI76_RS10045 0.36 +0.7
2,128,900 - BWI76_RS10045 0.48 +0.9
2,129,803 + BWI76_RS10050 0.16 +2.0
2,129,811 - BWI76_RS10050 0.17 +0.2
2,129,980 + BWI76_RS10050 0.48 -1.5
2,130,069 - BWI76_RS10050 0.64 +0.0
2,130,140 + BWI76_RS10050 0.76 +0.3
2,130,164 + BWI76_RS10050 0.81 +0.8
2,130,246 + -0.2
2,130,784 + BWI76_RS10055 0.63 +1.0
2,130,866 + BWI76_RS10055 0.74 -0.5
2,131,088 + +0.4

Or see this region's nucleotide sequence