Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02260 and BWI76_RS02265 are separated by 149 nucleotides BWI76_RS02265 and BWI76_RS02270 are separated by 224 nucleotides
BWI76_RS02260: BWI76_RS02260 - DNA-binding protein, at 464,785 to 465,705
_RS02260
BWI76_RS02265: BWI76_RS02265 - putative transmembrane protein, at 465,855 to 466,535
_RS02265
BWI76_RS02270: BWI76_RS02270 - DSBA oxidoreductase, at 466,760 to 467,542
_RS02270
Position (kb)
465
466
467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 464.934 kb on + strand, within BWI76_RS02260 at 464.934 kb on + strand, within BWI76_RS02260 at 465.097 kb on - strand, within BWI76_RS02260 at 465.279 kb on + strand, within BWI76_RS02260 at 465.435 kb on + strand, within BWI76_RS02260 at 465.437 kb on + strand, within BWI76_RS02260 at 465.445 kb on - strand, within BWI76_RS02260 at 465.536 kb on - strand, within BWI76_RS02260 at 465.608 kb on - strand, within BWI76_RS02260 at 465.647 kb on - strand at 465.647 kb on - strand at 465.647 kb on - strand at 465.801 kb on - strand at 465.853 kb on + strand at 465.907 kb on - strand at 465.907 kb on - strand at 465.925 kb on - strand, within BWI76_RS02265 at 466.201 kb on - strand, within BWI76_RS02265 at 466.315 kb on - strand, within BWI76_RS02265 at 466.421 kb on + strand, within BWI76_RS02265 at 466.429 kb on - strand, within BWI76_RS02265 at 466.434 kb on - strand, within BWI76_RS02265 at 466.589 kb on + strand at 466.589 kb on + strand at 466.589 kb on + strand at 466.591 kb on - strand at 466.597 kb on - strand at 466.606 kb on - strand at 466.614 kb on + strand at 466.695 kb on + strand at 466.740 kb on - strand at 466.859 kb on + strand, within BWI76_RS02270 at 466.926 kb on - strand, within BWI76_RS02270 at 466.958 kb on + strand, within BWI76_RS02270 at 466.971 kb on - strand, within BWI76_RS02270 at 466.993 kb on - strand, within BWI76_RS02270 at 467.193 kb on - strand, within BWI76_RS02270 at 467.256 kb on - strand, within BWI76_RS02270
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml remove 464,934 + BWI76_RS02260 0.16 -0.6 464,934 + BWI76_RS02260 0.16 -0.6 465,097 - BWI76_RS02260 0.34 +0.4 465,279 + BWI76_RS02260 0.54 -1.3 465,435 + BWI76_RS02260 0.71 -1.0 465,437 + BWI76_RS02260 0.71 -0.4 465,445 - BWI76_RS02260 0.72 -0.2 465,536 - BWI76_RS02260 0.82 +0.4 465,608 - BWI76_RS02260 0.89 -0.5 465,647 - +0.9 465,647 - -1.6 465,647 - -1.0 465,801 - +0.2 465,853 + -1.3 465,907 - -0.4 465,907 - -0.4 465,925 - BWI76_RS02265 0.10 +2.0 466,201 - BWI76_RS02265 0.51 +0.5 466,315 - BWI76_RS02265 0.68 +0.5 466,421 + BWI76_RS02265 0.83 -0.5 466,429 - BWI76_RS02265 0.84 -0.5 466,434 - BWI76_RS02265 0.85 +2.4 466,589 + -0.4 466,589 + +0.0 466,589 + -0.3 466,591 - +1.0 466,597 - -0.4 466,606 - -1.6 466,614 + -0.9 466,695 + +1.4 466,740 - -0.6 466,859 + BWI76_RS02270 0.13 +0.4 466,926 - BWI76_RS02270 0.21 +0.6 466,958 + BWI76_RS02270 0.25 +1.6 466,971 - BWI76_RS02270 0.27 +1.2 466,993 - BWI76_RS02270 0.30 +0.8 467,193 - BWI76_RS02270 0.55 -2.2 467,256 - BWI76_RS02270 0.63 -0.4
Or see this region's nucleotide sequence