Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22110 and BWI76_RS22115 are separated by 57 nucleotides BWI76_RS22115 and BWI76_RS22120 are separated by 165 nucleotides BWI76_RS22120 and BWI76_RS22125 are separated by 66 nucleotides BWI76_RS22125 and BWI76_RS22130 overlap by 4 nucleotides
BWI76_RS22110: BWI76_RS22110 - putative helix-turn-helix AraC-type transcriptional regulator, at 4,616,339 to 4,617,106
_RS22110
BWI76_RS22115: BWI76_RS22115 - S-ribosylhomocysteine lyase, at 4,617,164 to 4,617,679
_RS22115
BWI76_RS22120: BWI76_RS22120 - glutamate--cysteine ligase, at 4,617,845 to 4,619,401
_RS22120
BWI76_RS22125: BWI76_RS22125 - membrane protein, at 4,619,468 to 4,619,896
_RS22125
BWI76_RS22130: BWI76_RS22130 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 4,619,893 to 4,620,459
_RS22130
Position (kb) 4617
4618
4619
4620 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4616.860 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.969 kb on - strand, within BWI76_RS22110 at 4617.301 kb on + strand, within BWI76_RS22115 at 4617.473 kb on + strand, within BWI76_RS22115 at 4617.598 kb on - strand, within BWI76_RS22115 at 4617.625 kb on + strand, within BWI76_RS22115 at 4617.671 kb on - strand at 4617.737 kb on + strand at 4617.755 kb on - strand at 4617.987 kb on - strand at 4618.294 kb on + strand, within BWI76_RS22120 at 4618.434 kb on - strand, within BWI76_RS22120 at 4618.677 kb on + strand, within BWI76_RS22120 at 4618.718 kb on - strand, within BWI76_RS22120 at 4618.821 kb on - strand, within BWI76_RS22120 at 4618.821 kb on - strand, within BWI76_RS22120 at 4618.994 kb on + strand at 4619.108 kb on - strand, within BWI76_RS22120 at 4619.159 kb on + strand, within BWI76_RS22120 at 4619.160 kb on - strand, within BWI76_RS22120 at 4619.388 kb on - strand at 4619.594 kb on - strand, within BWI76_RS22125 at 4619.772 kb on + strand, within BWI76_RS22125 at 4619.780 kb on - strand, within BWI76_RS22125 at 4619.847 kb on - strand, within BWI76_RS22125 at 4619.881 kb on + strand at 4619.941 kb on + strand at 4620.018 kb on - strand, within BWI76_RS22130 at 4620.032 kb on + strand, within BWI76_RS22130 at 4620.184 kb on - strand, within BWI76_RS22130 at 4620.188 kb on + strand, within BWI76_RS22130
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 4,616,860 + BWI76_RS22110 0.68 +0.2 4,616,887 + BWI76_RS22110 0.71 -1.0 4,616,887 + BWI76_RS22110 0.71 -1.3 4,616,887 + BWI76_RS22110 0.71 +0.6 4,616,895 - BWI76_RS22110 0.72 +0.0 4,616,895 - BWI76_RS22110 0.72 +1.5 4,616,969 - BWI76_RS22110 0.82 -0.7 4,617,301 + BWI76_RS22115 0.27 -1.6 4,617,473 + BWI76_RS22115 0.60 -0.5 4,617,598 - BWI76_RS22115 0.84 -1.2 4,617,625 + BWI76_RS22115 0.89 -0.6 4,617,671 - -3.6 4,617,737 + +0.9 4,617,755 - +0.6 4,617,987 - -1.4 4,618,294 + BWI76_RS22120 0.29 +0.6 4,618,434 - BWI76_RS22120 0.38 -3.7 4,618,677 + BWI76_RS22120 0.53 -2.7 4,618,718 - BWI76_RS22120 0.56 -4.0 4,618,821 - BWI76_RS22120 0.63 -1.6 4,618,821 - BWI76_RS22120 0.63 -1.6 4,618,994 + -0.0 4,619,108 - BWI76_RS22120 0.81 +0.2 4,619,159 + BWI76_RS22120 0.84 +1.8 4,619,160 - BWI76_RS22120 0.84 -0.9 4,619,388 - -3.5 4,619,594 - BWI76_RS22125 0.29 -0.0 4,619,772 + BWI76_RS22125 0.71 -0.7 4,619,780 - BWI76_RS22125 0.73 -0.1 4,619,847 - BWI76_RS22125 0.88 -1.6 4,619,881 + -0.6 4,619,941 + -0.8 4,620,018 - BWI76_RS22130 0.22 -1.7 4,620,032 + BWI76_RS22130 0.25 -2.3 4,620,184 - BWI76_RS22130 0.51 -2.0 4,620,188 + BWI76_RS22130 0.52 -1.8
Or see this region's nucleotide sequence