Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14315 and BWI76_RS14320 are separated by 1 nucleotides BWI76_RS14320 and BWI76_RS14325 are separated by 7 nucleotides BWI76_RS14325 and BWI76_RS14330 are separated by 18 nucleotides
BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892
_RS14315
BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829
_RS14320
BWI76_RS14325: BWI76_RS14325 - maturase, at 2,992,837 to 2,992,957
_RS14325
BWI76_RS14330: BWI76_RS14330 - IS3 family transposase, at 2,992,976 to 2,993,797
_RS14330
Position (kb)
2992
2993 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2992.011 kb on + strand, within BWI76_RS14320 at 2992.114 kb on + strand, within BWI76_RS14320 at 2992.201 kb on + strand, within BWI76_RS14320 at 2992.293 kb on + strand at 2992.293 kb on + strand, within BWI76_RS14320 at 2992.395 kb on + strand, within BWI76_RS14320 at 2992.830 kb on + strand at 2992.894 kb on + strand, within BWI76_RS14325 at 2992.929 kb on - strand, within BWI76_RS14325 at 2992.932 kb on + strand, within BWI76_RS14325 at 2992.939 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.953 kb on + strand at 2992.989 kb on + strand at 2992.989 kb on + strand at 2993.024 kb on + strand at 2993.078 kb on - strand, within BWI76_RS14330 at 2993.080 kb on - strand at 2993.116 kb on + strand, within BWI76_RS14330 at 2993.159 kb on + strand, within BWI76_RS14330 at 2993.167 kb on - strand, within BWI76_RS14330 at 2993.191 kb on + strand, within BWI76_RS14330 at 2993.199 kb on - strand, within BWI76_RS14330 at 2993.224 kb on - strand, within BWI76_RS14330 at 2993.227 kb on + strand, within BWI76_RS14330 at 2993.227 kb on - strand, within BWI76_RS14330 at 2993.234 kb on - strand, within BWI76_RS14330 at 2993.235 kb on - strand, within BWI76_RS14330 at 2993.236 kb on + strand, within BWI76_RS14330 at 2993.236 kb on + strand, within BWI76_RS14330 at 2993.244 kb on - strand, within BWI76_RS14330 at 2993.317 kb on + strand, within BWI76_RS14330 at 2993.350 kb on + strand, within BWI76_RS14330 at 2993.418 kb on - strand at 2993.426 kb on - strand, within BWI76_RS14330 at 2993.467 kb on - strand, within BWI76_RS14330 at 2993.473 kb on + strand, within BWI76_RS14330 at 2993.749 kb on - strand at 2993.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 2,992,011 + BWI76_RS14320 0.12 -2.6 2,992,114 + BWI76_RS14320 0.24 +1.0 2,992,201 + BWI76_RS14320 0.33 -0.6 2,992,293 + +1.9 2,992,293 + BWI76_RS14320 0.43 -2.0 2,992,395 + BWI76_RS14320 0.54 -1.8 2,992,830 + -2.8 2,992,894 + BWI76_RS14325 0.47 -1.8 2,992,929 - BWI76_RS14325 0.76 -1.3 2,992,932 + BWI76_RS14325 0.79 -2.0 2,992,939 + BWI76_RS14325 0.84 -1.3 2,992,945 + BWI76_RS14325 0.89 -2.1 2,992,945 + BWI76_RS14325 0.89 -0.9 2,992,945 + BWI76_RS14325 0.89 +1.7 2,992,953 + -0.7 2,992,989 + -2.0 2,992,989 + +0.6 2,993,024 + +0.9 2,993,078 - BWI76_RS14330 0.12 +1.0 2,993,080 - +1.3 2,993,116 + BWI76_RS14330 0.17 +0.1 2,993,159 + BWI76_RS14330 0.22 -1.0 2,993,167 - BWI76_RS14330 0.23 +0.0 2,993,191 + BWI76_RS14330 0.26 -2.1 2,993,199 - BWI76_RS14330 0.27 +3.4 2,993,224 - BWI76_RS14330 0.30 -1.0 2,993,227 + BWI76_RS14330 0.31 -3.7 2,993,227 - BWI76_RS14330 0.31 -1.0 2,993,234 - BWI76_RS14330 0.31 +1.1 2,993,235 - BWI76_RS14330 0.32 +2.7 2,993,236 + BWI76_RS14330 0.32 +0.4 2,993,236 + BWI76_RS14330 0.32 -0.8 2,993,244 - BWI76_RS14330 0.33 +0.3 2,993,317 + BWI76_RS14330 0.41 -3.9 2,993,350 + BWI76_RS14330 0.45 -1.8 2,993,418 - +0.0 2,993,426 - BWI76_RS14330 0.55 +1.0 2,993,467 - BWI76_RS14330 0.60 +0.0 2,993,473 + BWI76_RS14330 0.60 +1.0 2,993,749 - +0.7 2,993,918 - +0.5
Or see this region's nucleotide sequence