Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotides BWI76_RS13130 and BWI76_RS13135 overlap by 1 nucleotides BWI76_RS13135 and BWI76_RS13140 are separated by 28 nucleotides
BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349
_RS13125
BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761
_RS13130
BWI76_RS13135: BWI76_RS13135 - 3-oxoadipyl-CoA thiolase, at 2,738,761 to 2,739,966
_RS13135
BWI76_RS13140: BWI76_RS13140 - phenylacetate-CoA ligase, at 2,739,995 to 2,741,311
_RS13140
Position (kb)
2738
2739
2740 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2737.786 kb on + strand, within BWI76_RS13125 at 2737.794 kb on - strand, within BWI76_RS13125 at 2737.877 kb on + strand, within BWI76_RS13125 at 2738.546 kb on + strand, within BWI76_RS13130 at 2738.845 kb on - strand at 2738.935 kb on - strand, within BWI76_RS13135 at 2739.311 kb on - strand at 2739.452 kb on + strand, within BWI76_RS13135 at 2739.460 kb on - strand, within BWI76_RS13135 at 2739.928 kb on - strand at 2739.931 kb on + strand at 2739.931 kb on + strand at 2740.433 kb on + strand, within BWI76_RS13140 at 2740.799 kb on + strand, within BWI76_RS13140 at 2740.865 kb on + strand, within BWI76_RS13140
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 2,737,786 + BWI76_RS13125 0.60 +0.8 2,737,794 - BWI76_RS13125 0.61 +0.4 2,737,877 + BWI76_RS13125 0.67 -1.0 2,738,546 + BWI76_RS13130 0.49 -0.7 2,738,845 - -0.7 2,738,935 - BWI76_RS13135 0.14 -3.1 2,739,311 - -0.6 2,739,452 + BWI76_RS13135 0.57 -0.6 2,739,460 - BWI76_RS13135 0.58 +0.1 2,739,928 - +1.0 2,739,931 + -0.2 2,739,931 + -0.7 2,740,433 + BWI76_RS13140 0.33 +0.0 2,740,799 + BWI76_RS13140 0.61 -0.1 2,740,865 + BWI76_RS13140 0.66 -4.5
Or see this region's nucleotide sequence