Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotides BWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides BWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotides
BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369
_RS13110
BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133
_RS13115
BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924
_RS13120
BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349
_RS13125
Position (kb)
2736
2737 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2735.337 kb on + strand at 2735.375 kb on - strand at 2736.150 kb on - strand at 2736.166 kb on - strand at 2736.758 kb on + strand, within BWI76_RS13120 at 2736.963 kb on - strand at 2737.662 kb on + strand, within BWI76_RS13125 at 2737.786 kb on + strand, within BWI76_RS13125 at 2737.794 kb on - strand, within BWI76_RS13125 at 2737.877 kb on + strand, within BWI76_RS13125
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 2,735,337 + -5.1 2,735,375 - +0.8 2,736,150 - +0.9 2,736,166 - +0.4 2,736,758 + BWI76_RS13120 0.79 +2.7 2,736,963 - +1.6 2,737,662 + BWI76_RS13125 0.52 +1.8 2,737,786 + BWI76_RS13125 0.60 +0.8 2,737,794 - BWI76_RS13125 0.61 +0.4 2,737,877 + BWI76_RS13125 0.67 -1.0
Or see this region's nucleotide sequence