Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13100 and BWI76_RS13105 are separated by 12 nucleotides BWI76_RS13105 and BWI76_RS13110 are separated by 7 nucleotides BWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotides BWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides
BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781
_RS13100
BWI76_RS13105: BWI76_RS13105 - phenylacetate-CoA oxygenase subunit PaaJ, at 2,733,794 to 2,734,291
_RS13105
BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369
_RS13110
BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133
_RS13115
BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924
_RS13120
Position (kb)
2734
2735
2736 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2733.362 kb on + strand, within BWI76_RS13100 at 2733.402 kb on + strand, within BWI76_RS13100 at 2733.453 kb on + strand, within BWI76_RS13100 at 2733.541 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.765 kb on - strand at 2734.083 kb on + strand, within BWI76_RS13105 at 2734.176 kb on - strand, within BWI76_RS13105 at 2734.235 kb on + strand, within BWI76_RS13105 at 2734.454 kb on + strand, within BWI76_RS13110 at 2734.932 kb on - strand, within BWI76_RS13110 at 2735.127 kb on - strand, within BWI76_RS13110 at 2735.337 kb on + strand at 2735.375 kb on - strand at 2736.150 kb on - strand at 2736.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 2,733,362 + BWI76_RS13100 0.44 -5.2 2,733,402 + BWI76_RS13100 0.50 +0.4 2,733,453 + BWI76_RS13100 0.56 +0.2 2,733,541 + BWI76_RS13100 0.68 +1.0 2,733,580 + BWI76_RS13100 0.73 -2.0 2,733,580 + BWI76_RS13100 0.73 +0.9 2,733,765 - +0.1 2,734,083 + BWI76_RS13105 0.58 -0.1 2,734,176 - BWI76_RS13105 0.77 +0.1 2,734,235 + BWI76_RS13105 0.89 -0.8 2,734,454 + BWI76_RS13110 0.14 +0.1 2,734,932 - BWI76_RS13110 0.59 -4.8 2,735,127 - BWI76_RS13110 0.77 -0.3 2,735,337 + -5.1 2,735,375 - +0.8 2,736,150 - +0.9 2,736,166 - +0.4
Or see this region's nucleotide sequence