Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09430 and BWI76_RS09435 are separated by 6 nucleotides BWI76_RS09435 and BWI76_RS09440 are separated by 204 nucleotides BWI76_RS09440 and BWI76_RS09445 overlap by 17 nucleotides BWI76_RS09445 and BWI76_RS09450 are separated by 103 nucleotides
BWI76_RS09430: BWI76_RS09430 - hypothetical protein, at 1,976,797 to 1,977,180
_RS09430
BWI76_RS09435: BWI76_RS09435 - glutaredoxin, GrxA family, at 1,977,187 to 1,977,450
_RS09435
BWI76_RS09440: BWI76_RS09440 - hypothetical protein, at 1,977,655 to 1,977,945
_RS09440
BWI76_RS09445: BWI76_RS09445 - NADPH-dependent oxidoreductase, at 1,977,929 to 1,978,651
_RS09445
BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657
_RS09450
Position (kb) 1977
1978 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1976.675 kb on - strand at 1976.677 kb on + strand at 1976.678 kb on + strand at 1976.685 kb on - strand at 1976.742 kb on - strand at 1976.765 kb on + strand at 1976.809 kb on - strand at 1976.819 kb on - strand at 1976.819 kb on - strand at 1976.821 kb on + strand at 1976.821 kb on + strand at 1976.821 kb on + strand at 1976.829 kb on - strand at 1976.876 kb on - strand, within BWI76_RS09430 at 1976.885 kb on - strand, within BWI76_RS09430 at 1976.888 kb on + strand, within BWI76_RS09430 at 1976.901 kb on + strand, within BWI76_RS09430 at 1976.901 kb on + strand, within BWI76_RS09430 at 1976.901 kb on + strand, within BWI76_RS09430 at 1976.901 kb on + strand, within BWI76_RS09430 at 1976.902 kb on + strand, within BWI76_RS09430 at 1976.909 kb on - strand, within BWI76_RS09430 at 1976.909 kb on - strand, within BWI76_RS09430 at 1976.921 kb on - strand, within BWI76_RS09430 at 1977.094 kb on + strand, within BWI76_RS09430 at 1977.094 kb on + strand, within BWI76_RS09430 at 1977.096 kb on + strand, within BWI76_RS09430 at 1977.096 kb on + strand, within BWI76_RS09430 at 1977.102 kb on - strand, within BWI76_RS09430 at 1977.162 kb on + strand at 1977.460 kb on + strand at 1977.468 kb on - strand at 1977.468 kb on - strand at 1977.729 kb on - strand, within BWI76_RS09440 at 1977.893 kb on - strand, within BWI76_RS09440 at 1978.172 kb on + strand, within BWI76_RS09445 at 1978.232 kb on + strand, within BWI76_RS09445 at 1978.300 kb on + strand, within BWI76_RS09445 at 1978.633 kb on + strand at 1978.728 kb on + strand at 1978.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 1,976,675 - +0.0 1,976,677 + -0.7 1,976,678 + -0.5 1,976,685 - +0.9 1,976,742 - -2.0 1,976,765 + +3.6 1,976,809 - -0.4 1,976,819 - +0.9 1,976,819 - -0.5 1,976,821 + +0.2 1,976,821 + +0.5 1,976,821 + +1.5 1,976,829 - +0.3 1,976,876 - BWI76_RS09430 0.21 +0.2 1,976,885 - BWI76_RS09430 0.23 -3.6 1,976,888 + BWI76_RS09430 0.24 +0.9 1,976,901 + BWI76_RS09430 0.27 +0.4 1,976,901 + BWI76_RS09430 0.27 +1.3 1,976,901 + BWI76_RS09430 0.27 +0.6 1,976,901 + BWI76_RS09430 0.27 +0.7 1,976,902 + BWI76_RS09430 0.27 -0.3 1,976,909 - BWI76_RS09430 0.29 -3.8 1,976,909 - BWI76_RS09430 0.29 -1.8 1,976,921 - BWI76_RS09430 0.32 +1.0 1,977,094 + BWI76_RS09430 0.77 +0.5 1,977,094 + BWI76_RS09430 0.77 -2.8 1,977,096 + BWI76_RS09430 0.78 -2.0 1,977,096 + BWI76_RS09430 0.78 -0.4 1,977,102 - BWI76_RS09430 0.79 -4.5 1,977,162 + -2.0 1,977,460 + +0.6 1,977,468 - +0.6 1,977,468 - +0.8 1,977,729 - BWI76_RS09440 0.25 +0.1 1,977,893 - BWI76_RS09440 0.82 +1.2 1,978,172 + BWI76_RS09445 0.34 +0.9 1,978,232 + BWI76_RS09445 0.42 +1.0 1,978,300 + BWI76_RS09445 0.51 +0.3 1,978,633 + -1.6 1,978,728 + -0.3 1,978,787 + -0.4
Or see this region's nucleotide sequence