Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03840 and BWI76_RS03845 are separated by 70 nucleotides BWI76_RS03845 and BWI76_RS03850 are separated by 10 nucleotides BWI76_RS03850 and BWI76_RS03855 are separated by 136 nucleotides
BWI76_RS03840: BWI76_RS03840 - 4-hydroxyphenylacetate permease, at 794,732 to 796,108
_RS03840
BWI76_RS03845: BWI76_RS03845 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase, at 796,179 to 796,971
_RS03845
BWI76_RS03850: BWI76_RS03850 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 796,982 to 797,785
_RS03850
BWI76_RS03855: BWI76_RS03855 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 797,922 to 798,302
_RS03855
Position (kb)
796
797 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 795.315 kb on + strand, within BWI76_RS03840 at 795.351 kb on - strand, within BWI76_RS03840 at 795.656 kb on - strand, within BWI76_RS03840 at 795.700 kb on + strand, within BWI76_RS03840 at 795.724 kb on + strand, within BWI76_RS03840 at 795.745 kb on - strand, within BWI76_RS03840 at 795.875 kb on - strand, within BWI76_RS03840 at 795.875 kb on - strand, within BWI76_RS03840 at 796.106 kb on + strand at 796.161 kb on + strand at 796.299 kb on + strand, within BWI76_RS03845 at 796.307 kb on - strand, within BWI76_RS03845 at 796.401 kb on + strand, within BWI76_RS03845 at 796.553 kb on + strand, within BWI76_RS03845 at 796.780 kb on + strand, within BWI76_RS03845 at 796.949 kb on - strand at 797.175 kb on - strand, within BWI76_RS03850 at 797.241 kb on - strand, within BWI76_RS03850 at 797.440 kb on - strand, within BWI76_RS03850 at 797.544 kb on + strand, within BWI76_RS03850 at 797.552 kb on - strand at 797.769 kb on - strand at 797.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 795,315 + BWI76_RS03840 0.42 +0.1 795,351 - BWI76_RS03840 0.45 -0.6 795,656 - BWI76_RS03840 0.67 -0.2 795,700 + BWI76_RS03840 0.70 -0.6 795,724 + BWI76_RS03840 0.72 -0.1 795,745 - BWI76_RS03840 0.74 +0.4 795,875 - BWI76_RS03840 0.83 +0.5 795,875 - BWI76_RS03840 0.83 -0.8 796,106 + +1.0 796,161 + -1.4 796,299 + BWI76_RS03845 0.15 -0.6 796,307 - BWI76_RS03845 0.16 -2.4 796,401 + BWI76_RS03845 0.28 +0.0 796,553 + BWI76_RS03845 0.47 -2.0 796,780 + BWI76_RS03845 0.76 -0.6 796,949 - +1.1 797,175 - BWI76_RS03850 0.24 +0.7 797,241 - BWI76_RS03850 0.32 -1.0 797,440 - BWI76_RS03850 0.57 +0.5 797,544 + BWI76_RS03850 0.70 -0.2 797,552 - +1.0 797,769 - +0.0 797,849 - +2.0
Or see this region's nucleotide sequence