Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03275 and BWI76_RS03280 are separated by 294 nucleotides BWI76_RS03280 and BWI76_RS03285 are separated by 61 nucleotides BWI76_RS03285 and BWI76_RS03290 overlap by 35 nucleotides BWI76_RS03290 and BWI76_RS03295 are separated by 72 nucleotides
BWI76_RS03275: BWI76_RS03275 - cyclomaltodextrin glucanotransferase, at 676,215 to 678,182
_RS03275
BWI76_RS03280: BWI76_RS03280 - IS630 family transposase, at 678,477 to 678,791
_RS03280
BWI76_RS03285: BWI76_RS03285 - hypothetical protein, at 678,853 to 679,071
_RS03285
BWI76_RS03290: BWI76_RS03290 - hypothetical protein, at 679,037 to 679,962
_RS03290
BWI76_RS03295: BWI76_RS03295 - transposase, at 680,035 to 680,298
_RS03295
Position (kb) 678
679
680 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 677.862 kb on + strand, within BWI76_RS03275 at 677.862 kb on + strand, within BWI76_RS03275 at 677.862 kb on + strand, within BWI76_RS03275 at 677.879 kb on + strand, within BWI76_RS03275 at 677.881 kb on + strand, within BWI76_RS03275 at 677.881 kb on + strand, within BWI76_RS03275 at 677.881 kb on + strand, within BWI76_RS03275 at 677.881 kb on + strand, within BWI76_RS03275 at 677.881 kb on + strand, within BWI76_RS03275 at 677.882 kb on + strand, within BWI76_RS03275 at 677.882 kb on + strand, within BWI76_RS03275 at 677.882 kb on + strand, within BWI76_RS03275 at 677.882 kb on + strand, within BWI76_RS03275 at 677.882 kb on + strand, within BWI76_RS03275 at 677.882 kb on + strand, within BWI76_RS03275 at 677.884 kb on + strand, within BWI76_RS03275 at 677.889 kb on + strand, within BWI76_RS03275 at 677.889 kb on - strand, within BWI76_RS03275 at 677.889 kb on - strand, within BWI76_RS03275 at 677.889 kb on - strand, within BWI76_RS03275 at 677.889 kb on - strand, within BWI76_RS03275 at 677.889 kb on - strand, within BWI76_RS03275 at 677.889 kb on - strand, within BWI76_RS03275 at 677.892 kb on - strand, within BWI76_RS03275 at 677.896 kb on + strand, within BWI76_RS03275 at 677.896 kb on + strand, within BWI76_RS03275 at 677.896 kb on + strand, within BWI76_RS03275 at 677.918 kb on + strand, within BWI76_RS03275 at 677.976 kb on + strand, within BWI76_RS03275 at 677.982 kb on + strand, within BWI76_RS03275 at 678.023 kb on + strand at 678.023 kb on + strand at 678.029 kb on - strand at 678.037 kb on + strand at 678.037 kb on + strand at 678.037 kb on + strand at 678.044 kb on + strand at 678.044 kb on + strand at 678.044 kb on + strand at 678.056 kb on + strand at 678.066 kb on + strand at 678.066 kb on + strand at 678.074 kb on - strand at 678.074 kb on - strand at 678.074 kb on - strand at 678.104 kb on + strand at 678.106 kb on + strand at 678.111 kb on + strand at 678.140 kb on + strand at 678.148 kb on - strand at 678.151 kb on + strand at 678.155 kb on + strand at 678.279 kb on + strand at 678.291 kb on - strand at 678.291 kb on - strand at 678.296 kb on - strand at 678.305 kb on - strand at 678.307 kb on + strand at 678.307 kb on + strand at 678.307 kb on + strand at 678.307 kb on + strand at 678.312 kb on + strand at 678.312 kb on + strand at 678.312 kb on + strand at 678.312 kb on + strand at 678.315 kb on - strand at 678.315 kb on - strand at 678.315 kb on - strand at 678.315 kb on - strand at 678.320 kb on - strand at 678.331 kb on + strand at 678.331 kb on + strand at 678.339 kb on - strand at 678.344 kb on - strand at 678.345 kb on + strand at 678.352 kb on - strand at 678.362 kb on - strand at 678.378 kb on - strand at 678.409 kb on - strand at 678.411 kb on + strand at 678.419 kb on - strand at 678.419 kb on - strand at 678.420 kb on + strand at 678.424 kb on + strand at 678.424 kb on - strand at 678.429 kb on + strand at 678.446 kb on + strand at 678.467 kb on + strand at 678.467 kb on + strand at 678.467 kb on + strand at 678.476 kb on - strand at 678.480 kb on - strand at 678.487 kb on - strand at 678.490 kb on + strand at 678.490 kb on + strand at 678.490 kb on + strand at 678.492 kb on + strand at 678.492 kb on + strand at 678.492 kb on + strand at 678.492 kb on + strand at 678.492 kb on + strand at 678.492 kb on + strand at 678.493 kb on - strand at 678.497 kb on + strand at 678.498 kb on + strand at 678.498 kb on - strand at 678.500 kb on - strand at 678.500 kb on - strand at 678.500 kb on - strand at 678.500 kb on - strand at 678.500 kb on - strand at 678.500 kb on - strand at 678.500 kb on - strand at 678.512 kb on - strand, within BWI76_RS03280 at 678.559 kb on + strand, within BWI76_RS03280 at 678.565 kb on + strand, within BWI76_RS03280 at 678.571 kb on + strand, within BWI76_RS03280 at 678.571 kb on + strand, within BWI76_RS03280 at 678.571 kb on + strand, within BWI76_RS03280 at 678.571 kb on + strand, within BWI76_RS03280 at 678.571 kb on + strand, within BWI76_RS03280 at 678.579 kb on - strand, within BWI76_RS03280 at 678.579 kb on - strand, within BWI76_RS03280 at 678.579 kb on - strand, within BWI76_RS03280 at 678.579 kb on - strand, within BWI76_RS03280 at 678.579 kb on - strand, within BWI76_RS03280 at 678.582 kb on + strand, within BWI76_RS03280 at 678.595 kb on - strand, within BWI76_RS03280 at 678.614 kb on + strand, within BWI76_RS03280 at 678.616 kb on + strand, within BWI76_RS03280 at 678.655 kb on + strand, within BWI76_RS03280 at 678.664 kb on + strand, within BWI76_RS03280 at 678.672 kb on - strand, within BWI76_RS03280 at 678.672 kb on - strand, within BWI76_RS03280 at 678.687 kb on - strand, within BWI76_RS03280 at 678.697 kb on - strand, within BWI76_RS03280 at 678.747 kb on + strand, within BWI76_RS03280 at 678.767 kb on - strand at 678.815 kb on - strand at 678.826 kb on + strand at 678.826 kb on + strand at 678.827 kb on + strand at 678.849 kb on + strand at 678.851 kb on + strand at 678.859 kb on - strand at 678.941 kb on - strand, within BWI76_RS03285 at 678.976 kb on + strand, within BWI76_RS03285 at 678.976 kb on + strand, within BWI76_RS03285 at 678.977 kb on + strand, within BWI76_RS03285 at 678.985 kb on - strand, within BWI76_RS03285 at 678.985 kb on - strand, within BWI76_RS03285 at 678.985 kb on - strand, within BWI76_RS03285 at 678.991 kb on - strand, within BWI76_RS03285 at 679.074 kb on - strand at 679.074 kb on - strand at 679.117 kb on + strand at 679.117 kb on + strand at 679.137 kb on - strand, within BWI76_RS03290 at 679.137 kb on - strand, within BWI76_RS03290 at 679.145 kb on + strand, within BWI76_RS03290 at 679.187 kb on - strand, within BWI76_RS03290 at 679.241 kb on - strand, within BWI76_RS03290 at 679.251 kb on + strand, within BWI76_RS03290 at 679.269 kb on + strand, within BWI76_RS03290 at 679.272 kb on - strand at 679.369 kb on + strand, within BWI76_RS03290 at 679.507 kb on + strand, within BWI76_RS03290 at 679.546 kb on + strand, within BWI76_RS03290 at 679.657 kb on + strand, within BWI76_RS03290 at 679.657 kb on + strand, within BWI76_RS03290 at 679.880 kb on + strand at 679.980 kb on + strand at 679.989 kb on + strand at 679.998 kb on - strand at 680.006 kb on + strand at 680.015 kb on + strand at 680.020 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 677,862 + BWI76_RS03275 0.84 +0.9 677,862 + BWI76_RS03275 0.84 +3.3 677,862 + BWI76_RS03275 0.84 -2.7 677,879 + BWI76_RS03275 0.85 -3.2 677,881 + BWI76_RS03275 0.85 -0.6 677,881 + BWI76_RS03275 0.85 -0.6 677,881 + BWI76_RS03275 0.85 -2.2 677,881 + BWI76_RS03275 0.85 +0.4 677,881 + BWI76_RS03275 0.85 -2.0 677,882 + BWI76_RS03275 0.85 +2.8 677,882 + BWI76_RS03275 0.85 +0.3 677,882 + BWI76_RS03275 0.85 -1.2 677,882 + BWI76_RS03275 0.85 -3.1 677,882 + BWI76_RS03275 0.85 -0.1 677,882 + BWI76_RS03275 0.85 -1.8 677,884 + BWI76_RS03275 0.85 -0.6 677,889 + BWI76_RS03275 0.85 -1.3 677,889 - BWI76_RS03275 0.85 -1.0 677,889 - BWI76_RS03275 0.85 -1.0 677,889 - BWI76_RS03275 0.85 -1.6 677,889 - BWI76_RS03275 0.85 -1.8 677,889 - BWI76_RS03275 0.85 -0.6 677,889 - BWI76_RS03275 0.85 -0.6 677,892 - BWI76_RS03275 0.85 -1.0 677,896 + BWI76_RS03275 0.85 -1.8 677,896 + BWI76_RS03275 0.85 -0.2 677,896 + BWI76_RS03275 0.85 -1.3 677,918 + BWI76_RS03275 0.87 +1.0 677,976 + BWI76_RS03275 0.89 -1.6 677,982 + BWI76_RS03275 0.90 +2.0 678,023 + -0.3 678,023 + -0.0 678,029 - -0.9 678,037 + +2.1 678,037 + -0.3 678,037 + -2.2 678,044 + -2.9 678,044 + -1.9 678,044 + +1.6 678,056 + +1.0 678,066 + +0.3 678,066 + -1.3 678,074 - -0.8 678,074 - +4.0 678,074 - -1.0 678,104 + -0.8 678,106 + +0.3 678,111 + -0.2 678,140 + -0.6 678,148 - -1.0 678,151 + +0.6 678,155 + -0.4 678,279 + -2.9 678,291 - +0.3 678,291 - -0.2 678,296 - +1.0 678,305 - -1.0 678,307 + -0.7 678,307 + +0.4 678,307 + +0.9 678,307 + -3.4 678,312 + -0.2 678,312 + +1.3 678,312 + -2.2 678,312 + -1.6 678,315 - -0.7 678,315 - +1.5 678,315 - -0.7 678,315 - -2.4 678,320 - -0.7 678,331 + +3.6 678,331 + +0.1 678,339 - +1.0 678,344 - +1.0 678,345 + -1.3 678,352 - -2.0 678,362 - -0.4 678,378 - +1.1 678,409 - -0.1 678,411 + -0.3 678,419 - -3.0 678,419 - +0.0 678,420 + -1.6 678,424 + -2.3 678,424 - +0.4 678,429 + -3.0 678,446 + -0.5 678,467 + +1.5 678,467 + +0.1 678,467 + -0.6 678,476 - -0.6 678,480 - +1.0 678,487 - -0.5 678,490 + -3.5 678,490 + -0.1 678,490 + +3.5 678,492 + -4.3 678,492 + +0.2 678,492 + +0.7 678,492 + +1.7 678,492 + +0.1 678,492 + -0.2 678,493 - +0.6 678,497 + -3.3 678,498 + -1.5 678,498 - -0.4 678,500 - -1.0 678,500 - -3.7 678,500 - -0.3 678,500 - -0.6 678,500 - -3.0 678,500 - -2.7 678,500 - -1.6 678,512 - BWI76_RS03280 0.11 -5.1 678,559 + BWI76_RS03280 0.26 -0.6 678,565 + BWI76_RS03280 0.28 -2.6 678,571 + BWI76_RS03280 0.30 +0.5 678,571 + BWI76_RS03280 0.30 +0.9 678,571 + BWI76_RS03280 0.30 +1.0 678,571 + BWI76_RS03280 0.30 +0.1 678,571 + BWI76_RS03280 0.30 +1.0 678,579 - BWI76_RS03280 0.32 +1.6 678,579 - BWI76_RS03280 0.32 -0.3 678,579 - BWI76_RS03280 0.32 +0.2 678,579 - BWI76_RS03280 0.32 +0.6 678,579 - BWI76_RS03280 0.32 -0.3 678,582 + BWI76_RS03280 0.33 -3.2 678,595 - BWI76_RS03280 0.37 +1.2 678,614 + BWI76_RS03280 0.43 -1.1 678,616 + BWI76_RS03280 0.44 -1.0 678,655 + BWI76_RS03280 0.57 -0.4 678,664 + BWI76_RS03280 0.59 +0.0 678,672 - BWI76_RS03280 0.62 -2.0 678,672 - BWI76_RS03280 0.62 +1.0 678,687 - BWI76_RS03280 0.67 -0.2 678,697 - BWI76_RS03280 0.70 -0.1 678,747 + BWI76_RS03280 0.86 +1.6 678,767 - -0.4 678,815 - -1.5 678,826 + -1.3 678,826 + -0.6 678,827 + +0.1 678,849 + +0.0 678,851 + +1.0 678,859 - +2.6 678,941 - BWI76_RS03285 0.40 -0.8 678,976 + BWI76_RS03285 0.56 +0.4 678,976 + BWI76_RS03285 0.56 -3.4 678,977 + BWI76_RS03285 0.57 +0.0 678,985 - BWI76_RS03285 0.60 -0.6 678,985 - BWI76_RS03285 0.60 -0.5 678,985 - BWI76_RS03285 0.60 -1.7 678,991 - BWI76_RS03285 0.63 -0.4 679,074 - +0.5 679,074 - -1.3 679,117 + -0.3 679,117 + -2.0 679,137 - BWI76_RS03290 0.11 -0.9 679,137 - BWI76_RS03290 0.11 -2.4 679,145 + BWI76_RS03290 0.12 +0.9 679,187 - BWI76_RS03290 0.16 +0.0 679,241 - BWI76_RS03290 0.22 -0.0 679,251 + BWI76_RS03290 0.23 -2.2 679,269 + BWI76_RS03290 0.25 -3.8 679,272 - +0.0 679,369 + BWI76_RS03290 0.36 +0.2 679,507 + BWI76_RS03290 0.51 -1.6 679,546 + BWI76_RS03290 0.55 -0.4 679,657 + BWI76_RS03290 0.67 +1.1 679,657 + BWI76_RS03290 0.67 -1.5 679,880 + -4.3 679,980 + +0.9 679,989 + -0.3 679,998 - +0.8 680,006 + -1.4 680,015 + +1.0 680,020 + -3.6
Or see this region's nucleotide sequence