Experiment: MoLS4 without Folic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0052 and DVUA0053 are separated by 250 nucleotides DVUA0053 and DVUA0054 overlap by 4 nucleotides DVUA0054 and DVUA0055 overlap by 4 nucleotides DVUA0055 and DVUA0056 overlap by 11 nucleotides
DVUA0052: DVUA0052 - conserved hypothetical protein (TIGR), at 64,069 to 64,737
DVUA0052
DVUA0053: DVUA0053 - conserved hypothetical protein (TIGR), at 64,988 to 65,575
DVUA0053
DVUA0054: DVUA0054 - glycosyl transferase, group 1 family protein (TIGR), at 65,572 to 66,972
DVUA0054
DVUA0055: DVUA0055 - membrane protein, putative (TIGR), at 66,969 to 67,922
DVUA0055
DVUA0056: DVUA0056 - hypothetical protein (TIGR), at 67,912 to 68,532
DVUA0056
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 64.650 kb on + strand, within DVUA0052 at 64.701 kb on + strand at 64.701 kb on + strand at 64.709 kb on - strand at 64.714 kb on + strand at 64.717 kb on - strand at 64.724 kb on + strand at 64.732 kb on + strand at 64.732 kb on + strand at 64.740 kb on - strand at 64.768 kb on - strand at 64.779 kb on - strand at 64.779 kb on - strand at 64.781 kb on + strand at 64.835 kb on + strand at 65.030 kb on + strand at 65.035 kb on + strand at 65.038 kb on - strand at 65.043 kb on - strand at 65.121 kb on + strand, within DVUA0053 at 65.138 kb on + strand, within DVUA0053 at 65.153 kb on + strand, within DVUA0053 at 65.161 kb on - strand, within DVUA0053 at 65.259 kb on - strand, within DVUA0053 at 65.264 kb on - strand, within DVUA0053 at 65.327 kb on + strand, within DVUA0053 at 65.444 kb on + strand, within DVUA0053 at 65.452 kb on - strand, within DVUA0053 at 65.516 kb on + strand, within DVUA0053 at 65.597 kb on + strand at 65.605 kb on - strand at 65.611 kb on - strand at 65.637 kb on + strand at 65.649 kb on - strand at 65.895 kb on + strand, within DVUA0054 at 65.903 kb on - strand, within DVUA0054 at 65.903 kb on - strand, within DVUA0054 at 65.903 kb on - strand, within DVUA0054 at 65.921 kb on + strand, within DVUA0054 at 65.929 kb on - strand, within DVUA0054 at 65.980 kb on + strand, within DVUA0054 at 66.012 kb on + strand, within DVUA0054 at 66.045 kb on - strand, within DVUA0054 at 66.055 kb on - strand, within DVUA0054 at 66.147 kb on - strand, within DVUA0054 at 66.149 kb on + strand, within DVUA0054 at 66.221 kb on + strand, within DVUA0054 at 66.292 kb on + strand, within DVUA0054 at 66.344 kb on - strand, within DVUA0054 at 66.401 kb on - strand, within DVUA0054 at 66.413 kb on + strand, within DVUA0054 at 66.464 kb on - strand, within DVUA0054 at 66.479 kb on - strand, within DVUA0054 at 66.487 kb on - strand, within DVUA0054 at 66.507 kb on + strand, within DVUA0054 at 66.521 kb on + strand, within DVUA0054 at 66.543 kb on + strand, within DVUA0054 at 66.574 kb on - strand, within DVUA0054 at 66.651 kb on - strand, within DVUA0054 at 66.743 kb on + strand, within DVUA0054 at 66.743 kb on + strand, within DVUA0054 at 66.751 kb on - strand, within DVUA0054 at 66.769 kb on - strand, within DVUA0054 at 66.907 kb on + strand at 66.921 kb on + strand at 67.007 kb on - strand at 67.061 kb on + strand at 67.102 kb on + strand, within DVUA0055 at 67.102 kb on + strand, within DVUA0055 at 67.105 kb on + strand, within DVUA0055 at 67.108 kb on - strand, within DVUA0055 at 67.117 kb on + strand, within DVUA0055 at 67.125 kb on - strand, within DVUA0055 at 67.137 kb on + strand, within DVUA0055 at 67.172 kb on - strand, within DVUA0055 at 67.231 kb on + strand, within DVUA0055 at 67.231 kb on + strand, within DVUA0055 at 67.239 kb on - strand, within DVUA0055 at 67.239 kb on - strand, within DVUA0055 at 67.275 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.378 kb on - strand, within DVUA0055 at 67.378 kb on - strand, within DVUA0055 at 67.446 kb on + strand, within DVUA0055 at 67.651 kb on + strand, within DVUA0055 at 67.803 kb on + strand, within DVUA0055 at 67.803 kb on + strand, within DVUA0055 at 67.874 kb on + strand at 67.892 kb on - strand at 67.911 kb on + strand at 67.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Folic with Casamino acids (vit. assay) 0.05% remove 64,650 + DVUA0052 0.87 +0.5 64,701 + -0.1 64,701 + -0.1 64,709 - -0.8 64,714 + -0.3 64,717 - -0.4 64,724 + -1.0 64,732 + +0.1 64,732 + -0.1 64,740 - -0.1 64,768 - -0.3 64,779 - +0.2 64,779 - +0.1 64,781 + +0.9 64,835 + -1.0 65,030 + +0.1 65,035 + +0.0 65,038 - -0.3 65,043 - -0.1 65,121 + DVUA0053 0.23 -0.3 65,138 + DVUA0053 0.26 -0.4 65,153 + DVUA0053 0.28 -0.8 65,161 - DVUA0053 0.29 -0.3 65,259 - DVUA0053 0.46 +0.7 65,264 - DVUA0053 0.47 -0.4 65,327 + DVUA0053 0.58 +1.3 65,444 + DVUA0053 0.78 +0.0 65,452 - DVUA0053 0.79 +0.3 65,516 + DVUA0053 0.90 -0.4 65,597 + +0.7 65,605 - -0.5 65,611 - -0.5 65,637 + +0.0 65,649 - +0.1 65,895 + DVUA0054 0.23 -0.6 65,903 - DVUA0054 0.24 -2.1 65,903 - DVUA0054 0.24 -0.5 65,903 - DVUA0054 0.24 -0.3 65,921 + DVUA0054 0.25 +0.1 65,929 - DVUA0054 0.25 -0.7 65,980 + DVUA0054 0.29 -1.9 66,012 + DVUA0054 0.31 -0.2 66,045 - DVUA0054 0.34 +0.3 66,055 - DVUA0054 0.34 -1.5 66,147 - DVUA0054 0.41 -0.2 66,149 + DVUA0054 0.41 -0.1 66,221 + DVUA0054 0.46 -1.0 66,292 + DVUA0054 0.51 -0.8 66,344 - DVUA0054 0.55 -0.2 66,401 - DVUA0054 0.59 -0.5 66,413 + DVUA0054 0.60 -1.7 66,464 - DVUA0054 0.64 -0.9 66,479 - DVUA0054 0.65 +0.1 66,487 - DVUA0054 0.65 -0.7 66,507 + DVUA0054 0.67 -0.3 66,521 + DVUA0054 0.68 +0.3 66,543 + DVUA0054 0.69 -1.3 66,574 - DVUA0054 0.72 -1.0 66,651 - DVUA0054 0.77 -0.4 66,743 + DVUA0054 0.84 +1.9 66,743 + DVUA0054 0.84 -0.3 66,751 - DVUA0054 0.84 -0.2 66,769 - DVUA0054 0.85 -0.3 66,907 + -1.4 66,921 + -0.3 67,007 - -0.7 67,061 + -0.3 67,102 + DVUA0055 0.14 -0.6 67,102 + DVUA0055 0.14 -0.3 67,105 + DVUA0055 0.14 +0.2 67,108 - DVUA0055 0.15 -2.3 67,117 + DVUA0055 0.16 -0.2 67,125 - DVUA0055 0.16 -0.5 67,137 + DVUA0055 0.18 +0.3 67,172 - DVUA0055 0.21 -1.0 67,231 + DVUA0055 0.27 +0.1 67,231 + DVUA0055 0.27 +0.2 67,239 - DVUA0055 0.28 -0.1 67,239 - DVUA0055 0.28 -0.2 67,275 + DVUA0055 0.32 +0.3 67,370 + DVUA0055 0.42 -0.1 67,370 + DVUA0055 0.42 -0.3 67,370 + DVUA0055 0.42 +1.7 67,370 + DVUA0055 0.42 +0.0 67,378 - DVUA0055 0.43 -0.7 67,378 - DVUA0055 0.43 -0.4 67,446 + DVUA0055 0.50 +1.3 67,651 + DVUA0055 0.71 +0.2 67,803 + DVUA0055 0.87 -0.2 67,803 + DVUA0055 0.87 -0.3 67,874 + -1.3 67,892 - +0.3 67,911 + +0.1 67,966 + -0.8
Or see this region's nucleotide sequence