Experiment: MoLS4 without Folic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livH and DVU0549 are separated by 27 nucleotides DVU0549 and livG overlap by 1 nucleotides livG and livF overlap by 8 nucleotides
DVU0548: livH - high-affinity branched-chain amino acid ABC transporter, permease protein (TIGR), at 618,218 to 619,126
livH
DVU0549: DVU0549 - proline/arginine ABC transporter, permease component <small>(from data)</small>, at 619,154 to 620,377
DVU0549
DVU0550: livG - high-affinity branched-chain amino acid ABC transporter, ATP binding protein (TIGR), at 620,377 to 621,165
livG
DVU0551: livF - high-affinity branched-chain amino acid ABC transporter ATP-binding protein (TIGR), at 621,158 to 621,862
livF
Position (kb) 619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 618.292 kb on + strand at 618.292 kb on + strand at 618.297 kb on + strand at 618.333 kb on - strand, within livH at 618.449 kb on + strand, within livH at 618.464 kb on + strand, within livH at 618.464 kb on + strand, within livH at 618.501 kb on - strand, within livH at 618.521 kb on - strand, within livH at 618.588 kb on - strand, within livH at 618.699 kb on + strand, within livH at 618.706 kb on + strand, within livH at 618.716 kb on - strand, within livH at 618.722 kb on + strand, within livH at 618.739 kb on + strand, within livH at 618.744 kb on + strand, within livH at 618.747 kb on - strand, within livH at 618.747 kb on - strand, within livH at 618.747 kb on - strand, within livH at 618.777 kb on + strand, within livH at 618.801 kb on + strand, within livH at 618.815 kb on - strand, within livH at 618.924 kb on - strand, within livH at 619.060 kb on + strand at 619.113 kb on - strand at 619.245 kb on + strand at 619.361 kb on - strand, within DVU0549 at 619.457 kb on + strand, within DVU0549 at 619.471 kb on + strand, within DVU0549 at 619.518 kb on - strand, within DVU0549 at 619.518 kb on - strand, within DVU0549 at 619.615 kb on - strand, within DVU0549 at 619.616 kb on - strand, within DVU0549 at 619.660 kb on - strand, within DVU0549 at 619.660 kb on - strand, within DVU0549 at 619.678 kb on - strand, within DVU0549 at 619.717 kb on + strand, within DVU0549 at 619.794 kb on + strand, within DVU0549 at 619.877 kb on + strand, within DVU0549 at 619.965 kb on + strand, within DVU0549 at 620.000 kb on + strand, within DVU0549 at 620.007 kb on + strand, within DVU0549 at 620.091 kb on + strand, within DVU0549 at 620.095 kb on - strand, within DVU0549 at 620.100 kb on - strand, within DVU0549 at 620.184 kb on + strand, within DVU0549 at 620.192 kb on - strand, within DVU0549 at 620.322 kb on + strand at 620.455 kb on - strand at 620.459 kb on + strand, within livG at 620.481 kb on + strand, within livG at 620.667 kb on + strand, within livG at 620.797 kb on + strand, within livG at 620.826 kb on + strand, within livG at 620.850 kb on + strand, within livG at 620.850 kb on + strand, within livG at 620.850 kb on + strand, within livG at 620.858 kb on - strand, within livG at 620.858 kb on - strand, within livG at 620.858 kb on - strand, within livG at 620.916 kb on - strand, within livG at 621.000 kb on + strand, within livG at 621.044 kb on - strand, within livG at 621.098 kb on + strand at 621.101 kb on - strand at 621.130 kb on + strand at 621.167 kb on - strand at 621.167 kb on - strand at 621.177 kb on - strand at 621.177 kb on - strand at 621.221 kb on - strand at 621.280 kb on - strand, within livF at 621.280 kb on - strand, within livF at 621.287 kb on - strand, within livF at 621.311 kb on + strand, within livF at 621.321 kb on + strand, within livF
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Folic with Casamino acids (vit. assay) 0.05% remove 618,292 + +0.6 618,292 + +1.0 618,297 + +0.6 618,333 - livH DVU0548 0.13 -0.6 618,449 + livH DVU0548 0.25 +0.1 618,464 + livH DVU0548 0.27 +0.1 618,464 + livH DVU0548 0.27 +0.3 618,501 - livH DVU0548 0.31 -0.3 618,521 - livH DVU0548 0.33 -0.6 618,588 - livH DVU0548 0.41 -1.6 618,699 + livH DVU0548 0.53 -0.3 618,706 + livH DVU0548 0.54 +0.1 618,716 - livH DVU0548 0.55 -0.9 618,722 + livH DVU0548 0.55 -0.1 618,739 + livH DVU0548 0.57 +0.1 618,744 + livH DVU0548 0.58 -0.2 618,747 - livH DVU0548 0.58 -1.1 618,747 - livH DVU0548 0.58 -0.8 618,747 - livH DVU0548 0.58 -0.2 618,777 + livH DVU0548 0.61 -0.4 618,801 + livH DVU0548 0.64 -1.4 618,815 - livH DVU0548 0.66 -0.3 618,924 - livH DVU0548 0.78 +0.2 619,060 + -0.4 619,113 - -0.8 619,245 + +1.6 619,361 - DVU0549 0.17 -2.3 619,457 + DVU0549 0.25 -1.1 619,471 + DVU0549 0.26 -0.5 619,518 - DVU0549 0.30 -0.0 619,518 - DVU0549 0.30 -1.5 619,615 - DVU0549 0.38 -1.6 619,616 - DVU0549 0.38 -0.2 619,660 - DVU0549 0.41 -1.1 619,660 - DVU0549 0.41 -1.9 619,678 - DVU0549 0.43 -1.5 619,717 + DVU0549 0.46 -0.7 619,794 + DVU0549 0.52 -2.5 619,877 + DVU0549 0.59 -0.2 619,965 + DVU0549 0.66 -2.9 620,000 + DVU0549 0.69 -1.2 620,007 + DVU0549 0.70 -0.0 620,091 + DVU0549 0.77 -0.5 620,095 - DVU0549 0.77 -0.3 620,100 - DVU0549 0.77 +0.1 620,184 + DVU0549 0.84 +0.8 620,192 - DVU0549 0.85 -1.0 620,322 + -0.5 620,455 - -0.5 620,459 + livG DVU0550 0.10 -0.2 620,481 + livG DVU0550 0.13 -0.9 620,667 + livG DVU0550 0.37 -0.2 620,797 + livG DVU0550 0.53 -0.4 620,826 + livG DVU0550 0.57 -0.6 620,850 + livG DVU0550 0.60 +0.3 620,850 + livG DVU0550 0.60 -0.4 620,850 + livG DVU0550 0.60 +0.2 620,858 - livG DVU0550 0.61 -0.2 620,858 - livG DVU0550 0.61 -0.8 620,858 - livG DVU0550 0.61 +0.2 620,916 - livG DVU0550 0.68 -0.2 621,000 + livG DVU0550 0.79 -0.2 621,044 - livG DVU0550 0.85 -1.6 621,098 + -0.4 621,101 - +0.1 621,130 + -0.1 621,167 - +0.7 621,167 - -0.4 621,177 - -0.3 621,177 - -0.2 621,221 - +0.5 621,280 - livF DVU0551 0.17 +2.6 621,280 - livF DVU0551 0.17 -1.2 621,287 - livF DVU0551 0.18 -0.9 621,311 + livF DVU0551 0.22 -0.5 621,321 + livF DVU0551 0.23 -1.1
Or see this region's nucleotide sequence