Experiment: MoLS4 without Folic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobB-2 and cobH are separated by 8 nucleotides cobH and deaD are separated by 108 nucleotides
DVU3086: cobB-2 - cobyrinic acid a,c-diamide synthase (TIGR), at 3,227,312 to 3,229,135
cobB-2
DVU3087: cobH - precorrin-8X methylmutase (TIGR), at 3,229,144 to 3,229,785
cobH
DVU3088: deaD - ATP-dependent RNA helicase, DEAD/DEAH box family (TIGR), at 3,229,894 to 3,231,627
deaD
Position (kb)
3229
3230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3228.215 kb on - strand, within cobB-2 at 3228.238 kb on + strand, within cobB-2 at 3228.246 kb on - strand, within cobB-2 at 3228.246 kb on - strand, within cobB-2 at 3228.283 kb on - strand, within cobB-2 at 3228.285 kb on + strand, within cobB-2 at 3228.300 kb on - strand, within cobB-2 at 3228.300 kb on - strand, within cobB-2 at 3228.486 kb on - strand, within cobB-2 at 3228.523 kb on - strand, within cobB-2 at 3228.563 kb on + strand, within cobB-2 at 3228.571 kb on - strand, within cobB-2 at 3228.580 kb on + strand, within cobB-2 at 3228.618 kb on + strand, within cobB-2 at 3228.634 kb on + strand, within cobB-2 at 3228.650 kb on - strand, within cobB-2 at 3228.706 kb on + strand, within cobB-2 at 3228.804 kb on + strand, within cobB-2 at 3228.876 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.910 kb on - strand, within cobB-2 at 3228.918 kb on + strand, within cobB-2 at 3228.921 kb on + strand, within cobB-2 at 3228.983 kb on + strand at 3228.991 kb on - strand at 3229.020 kb on + strand at 3229.020 kb on + strand at 3229.020 kb on + strand at 3229.104 kb on - strand at 3229.118 kb on + strand at 3229.128 kb on + strand at 3229.153 kb on + strand at 3229.276 kb on - strand, within cobH at 3229.281 kb on - strand, within cobH at 3229.281 kb on - strand, within cobH at 3229.281 kb on - strand, within cobH at 3229.312 kb on + strand, within cobH at 3229.312 kb on + strand, within cobH at 3229.408 kb on - strand, within cobH at 3229.423 kb on + strand, within cobH at 3229.489 kb on + strand, within cobH at 3229.579 kb on - strand, within cobH at 3229.604 kb on - strand, within cobH at 3229.610 kb on + strand, within cobH at 3229.613 kb on - strand, within cobH at 3229.618 kb on - strand, within cobH at 3229.647 kb on - strand, within cobH at 3229.691 kb on + strand, within cobH at 3229.691 kb on + strand, within cobH at 3229.694 kb on - strand, within cobH at 3229.852 kb on + strand at 3229.859 kb on - strand at 3229.898 kb on + strand at 3229.966 kb on + strand at 3230.011 kb on - strand at 3230.044 kb on + strand at 3230.059 kb on + strand at 3230.092 kb on + strand, within deaD at 3230.093 kb on - strand, within deaD at 3230.097 kb on + strand, within deaD at 3230.100 kb on - strand, within deaD at 3230.136 kb on - strand, within deaD at 3230.175 kb on - strand, within deaD at 3230.178 kb on + strand, within deaD at 3230.197 kb on + strand, within deaD at 3230.308 kb on + strand, within deaD at 3230.337 kb on + strand, within deaD at 3230.349 kb on + strand, within deaD at 3230.406 kb on + strand, within deaD at 3230.421 kb on + strand, within deaD at 3230.448 kb on + strand, within deaD at 3230.462 kb on + strand, within deaD at 3230.566 kb on + strand, within deaD at 3230.567 kb on + strand, within deaD at 3230.682 kb on + strand, within deaD at 3230.689 kb on + strand, within deaD at 3230.747 kb on - strand, within deaD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Folic with Casamino acids (vit. assay) 0.05% remove 3,228,215 - cobB-2 DVU3086 0.50 +0.5 3,228,238 + cobB-2 DVU3086 0.51 -0.2 3,228,246 - cobB-2 DVU3086 0.51 -0.7 3,228,246 - cobB-2 DVU3086 0.51 +1.5 3,228,283 - cobB-2 DVU3086 0.53 -2.0 3,228,285 + cobB-2 DVU3086 0.53 +0.2 3,228,300 - cobB-2 DVU3086 0.54 -1.0 3,228,300 - cobB-2 DVU3086 0.54 -0.7 3,228,486 - cobB-2 DVU3086 0.64 -2.5 3,228,523 - cobB-2 DVU3086 0.66 +0.5 3,228,563 + cobB-2 DVU3086 0.69 -0.1 3,228,571 - cobB-2 DVU3086 0.69 -2.1 3,228,580 + cobB-2 DVU3086 0.70 -2.1 3,228,618 + cobB-2 DVU3086 0.72 -1.8 3,228,634 + cobB-2 DVU3086 0.72 +0.5 3,228,650 - cobB-2 DVU3086 0.73 -0.1 3,228,706 + cobB-2 DVU3086 0.76 -0.3 3,228,804 + cobB-2 DVU3086 0.82 -0.5 3,228,876 - cobB-2 DVU3086 0.86 -0.9 3,228,910 - cobB-2 DVU3086 0.88 +0.5 3,228,910 - cobB-2 DVU3086 0.88 -0.1 3,228,910 - cobB-2 DVU3086 0.88 +1.5 3,228,910 - cobB-2 DVU3086 0.88 -0.8 3,228,918 + cobB-2 DVU3086 0.88 -1.1 3,228,921 + cobB-2 DVU3086 0.88 -0.4 3,228,983 + -0.9 3,228,991 - -1.4 3,229,020 + -1.3 3,229,020 + +1.8 3,229,020 + +0.5 3,229,104 - +1.1 3,229,118 + -0.8 3,229,128 + -0.4 3,229,153 + -0.3 3,229,276 - cobH DVU3087 0.21 +0.2 3,229,281 - cobH DVU3087 0.21 +1.0 3,229,281 - cobH DVU3087 0.21 -0.5 3,229,281 - cobH DVU3087 0.21 +0.6 3,229,312 + cobH DVU3087 0.26 +0.1 3,229,312 + cobH DVU3087 0.26 +0.3 3,229,408 - cobH DVU3087 0.41 -2.9 3,229,423 + cobH DVU3087 0.43 -0.1 3,229,489 + cobH DVU3087 0.54 -0.8 3,229,579 - cobH DVU3087 0.68 -0.4 3,229,604 - cobH DVU3087 0.72 -0.9 3,229,610 + cobH DVU3087 0.73 -0.5 3,229,613 - cobH DVU3087 0.73 -0.1 3,229,618 - cobH DVU3087 0.74 -3.2 3,229,647 - cobH DVU3087 0.78 -0.2 3,229,691 + cobH DVU3087 0.85 -1.8 3,229,691 + cobH DVU3087 0.85 -1.4 3,229,694 - cobH DVU3087 0.86 -1.7 3,229,852 + -0.5 3,229,859 - +0.8 3,229,898 + -0.4 3,229,966 + -0.5 3,230,011 - -1.6 3,230,044 + -0.4 3,230,059 + -0.1 3,230,092 + deaD DVU3088 0.11 -0.9 3,230,093 - deaD DVU3088 0.11 -0.7 3,230,097 + deaD DVU3088 0.12 +0.3 3,230,100 - deaD DVU3088 0.12 -1.7 3,230,136 - deaD DVU3088 0.14 -1.1 3,230,175 - deaD DVU3088 0.16 +0.6 3,230,178 + deaD DVU3088 0.16 -0.1 3,230,197 + deaD DVU3088 0.17 -0.2 3,230,308 + deaD DVU3088 0.24 -0.3 3,230,337 + deaD DVU3088 0.26 -1.9 3,230,349 + deaD DVU3088 0.26 -0.8 3,230,406 + deaD DVU3088 0.30 -0.7 3,230,421 + deaD DVU3088 0.30 +1.0 3,230,448 + deaD DVU3088 0.32 -0.5 3,230,462 + deaD DVU3088 0.33 -0.2 3,230,566 + deaD DVU3088 0.39 -1.1 3,230,567 + deaD DVU3088 0.39 +0.7 3,230,682 + deaD DVU3088 0.45 -0.6 3,230,689 + deaD DVU3088 0.46 +1.5 3,230,747 - deaD DVU3088 0.49 -0.1
Or see this region's nucleotide sequence