Experiment: MoLS4 without Folic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysA-2 and lysX overlap by 1 nucleotides lysX and cpsA are separated by 599 nucleotides
DVU2566: lysA-2 - diaminopimelate decarboxylase (TIGR), at 2,678,811 to 2,680,076
lysA-2
DVU2567: lysX - predicted transcriptional regulator for lysine biosynthesis and transport (Dmitry Rodionov), at 2,680,076 to 2,680,780
lysX
DVU2568: cpsA - peptidase, M20/M25/M40 family (TIGR), at 2,681,380 to 2,682,618
cpsA
Position (kb)
2680
2681 Strain fitness (log2 ratio)
-2
-1
0
1 at 2679.679 kb on + strand, within lysA-2 at 2679.687 kb on - strand, within lysA-2 at 2679.735 kb on + strand, within lysA-2 at 2679.743 kb on - strand, within lysA-2 at 2679.832 kb on + strand, within lysA-2 at 2679.862 kb on + strand, within lysA-2 at 2679.862 kb on + strand, within lysA-2 at 2679.992 kb on - strand at 2679.993 kb on + strand at 2680.074 kb on - strand at 2680.126 kb on + strand at 2680.143 kb on + strand at 2680.223 kb on + strand, within lysX at 2680.563 kb on - strand, within lysX at 2680.606 kb on - strand, within lysX at 2680.697 kb on + strand, within lysX at 2680.698 kb on + strand, within lysX at 2680.719 kb on + strand at 2680.794 kb on + strand at 2680.795 kb on - strand at 2680.838 kb on + strand at 2680.849 kb on + strand at 2680.870 kb on + strand at 2680.880 kb on + strand at 2680.880 kb on + strand at 2680.880 kb on + strand at 2680.880 kb on + strand at 2680.888 kb on - strand at 2681.094 kb on + strand at 2681.123 kb on + strand at 2681.131 kb on - strand at 2681.131 kb on - strand at 2681.247 kb on + strand at 2681.261 kb on + strand at 2681.314 kb on - strand at 2681.316 kb on + strand at 2681.345 kb on + strand at 2681.400 kb on + strand at 2681.400 kb on + strand at 2681.434 kb on + strand at 2681.434 kb on + strand at 2681.442 kb on - strand at 2681.442 kb on - strand at 2681.442 kb on - strand at 2681.442 kb on - strand at 2681.442 kb on - strand at 2681.474 kb on - strand at 2681.488 kb on - strand at 2681.734 kb on - strand, within cpsA at 2681.757 kb on - strand, within cpsA at 2681.776 kb on + strand, within cpsA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Folic with Casamino acids (vit. assay) 0.05% remove 2,679,679 + lysA-2 DVU2566 0.69 +1.3 2,679,687 - lysA-2 DVU2566 0.69 +0.3 2,679,735 + lysA-2 DVU2566 0.73 +0.8 2,679,743 - lysA-2 DVU2566 0.74 +0.6 2,679,832 + lysA-2 DVU2566 0.81 -0.8 2,679,862 + lysA-2 DVU2566 0.83 +0.2 2,679,862 + lysA-2 DVU2566 0.83 +0.1 2,679,992 - -0.1 2,679,993 + -0.4 2,680,074 - -0.7 2,680,126 + +1.2 2,680,143 + -0.4 2,680,223 + lysX DVU2567 0.21 -1.9 2,680,563 - lysX DVU2567 0.69 -1.5 2,680,606 - lysX DVU2567 0.75 -0.4 2,680,697 + lysX DVU2567 0.88 -0.6 2,680,698 + lysX DVU2567 0.88 -1.0 2,680,719 + -0.6 2,680,794 + +1.1 2,680,795 - -0.0 2,680,838 + -0.5 2,680,849 + -0.1 2,680,870 + +0.9 2,680,880 + +0.1 2,680,880 + +0.3 2,680,880 + +0.6 2,680,880 + -0.4 2,680,888 - +0.2 2,681,094 + -0.3 2,681,123 + -0.1 2,681,131 - -0.0 2,681,131 - +0.0 2,681,247 + +0.1 2,681,261 + -0.2 2,681,314 - +0.8 2,681,316 + -1.6 2,681,345 + +0.1 2,681,400 + -0.6 2,681,400 + -0.2 2,681,434 + +0.3 2,681,434 + -0.4 2,681,442 - -0.4 2,681,442 - -0.0 2,681,442 - -1.9 2,681,442 - +0.1 2,681,442 - +0.0 2,681,474 - -0.3 2,681,488 - +0.4 2,681,734 - cpsA DVU2568 0.29 -0.2 2,681,757 - cpsA DVU2568 0.30 -0.9 2,681,776 + cpsA DVU2568 0.32 -0.0
Or see this region's nucleotide sequence