Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17250 and BWI76_RS17255 are separated by 176 nucleotides BWI76_RS17255 and BWI76_RS17260 are separated by 144 nucleotides BWI76_RS17260 and BWI76_RS17265 are separated by 93 nucleotides
BWI76_RS17250: BWI76_RS17250 - bifunctional acetaldehyde-CoA/alcohol dehydrogenase, at 3,574,176 to 3,576,851
_RS17250
BWI76_RS17255: BWI76_RS17255 - thymidine kinase, at 3,577,028 to 3,577,645
_RS17255
BWI76_RS17260: BWI76_RS17260 - hypothetical protein, at 3,577,790 to 3,578,108
_RS17260
BWI76_RS17265: BWI76_RS17265 - DNA-binding protein, at 3,578,202 to 3,578,609
_RS17265
Position (kb)
3577
3578 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3576.126 kb on + strand, within BWI76_RS17250 at 3576.208 kb on + strand, within BWI76_RS17250 at 3576.279 kb on + strand, within BWI76_RS17250 at 3576.284 kb on + strand, within BWI76_RS17250 at 3576.711 kb on - strand at 3576.797 kb on - strand at 3576.815 kb on + strand at 3576.823 kb on - strand at 3577.054 kb on - strand at 3577.203 kb on - strand, within BWI76_RS17255 at 3577.203 kb on - strand, within BWI76_RS17255 at 3577.301 kb on - strand, within BWI76_RS17255 at 3577.313 kb on - strand, within BWI76_RS17255 at 3577.338 kb on + strand, within BWI76_RS17255 at 3577.338 kb on + strand at 3577.338 kb on + strand, within BWI76_RS17255 at 3577.338 kb on + strand, within BWI76_RS17255 at 3577.339 kb on + strand, within BWI76_RS17255 at 3577.346 kb on - strand, within BWI76_RS17255 at 3577.346 kb on - strand, within BWI76_RS17255 at 3577.365 kb on - strand, within BWI76_RS17255 at 3577.368 kb on - strand, within BWI76_RS17255 at 3577.376 kb on - strand, within BWI76_RS17255 at 3577.382 kb on + strand, within BWI76_RS17255 at 3577.421 kb on - strand, within BWI76_RS17255 at 3577.421 kb on - strand, within BWI76_RS17255 at 3577.423 kb on - strand, within BWI76_RS17255 at 3577.425 kb on + strand, within BWI76_RS17255 at 3577.433 kb on - strand, within BWI76_RS17255 at 3577.463 kb on - strand, within BWI76_RS17255 at 3577.473 kb on + strand, within BWI76_RS17255 at 3577.481 kb on - strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.589 kb on - strand at 3577.593 kb on + strand at 3577.599 kb on - strand at 3577.602 kb on - strand at 3577.623 kb on - strand at 3577.659 kb on - strand at 3577.723 kb on + strand at 3577.728 kb on + strand at 3577.729 kb on - strand at 3577.740 kb on - strand at 3577.757 kb on - strand at 3577.759 kb on - strand at 3577.759 kb on - strand at 3577.773 kb on - strand at 3577.797 kb on + strand at 3577.802 kb on - strand at 3577.805 kb on - strand at 3577.816 kb on - strand at 3577.825 kb on + strand, within BWI76_RS17260 at 3577.840 kb on - strand, within BWI76_RS17260 at 3577.848 kb on - strand, within BWI76_RS17260 at 3577.848 kb on - strand, within BWI76_RS17260 at 3577.915 kb on - strand, within BWI76_RS17260 at 3577.945 kb on + strand, within BWI76_RS17260 at 3577.963 kb on - strand, within BWI76_RS17260 at 3577.963 kb on - strand, within BWI76_RS17260 at 3577.992 kb on - strand, within BWI76_RS17260 at 3578.014 kb on - strand, within BWI76_RS17260 at 3578.118 kb on + strand at 3578.119 kb on - strand at 3578.123 kb on + strand at 3578.123 kb on + strand at 3578.522 kb on + strand, within BWI76_RS17265
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x remove 3,576,126 + BWI76_RS17250 0.73 -0.2 3,576,208 + BWI76_RS17250 0.76 -0.3 3,576,279 + BWI76_RS17250 0.79 +0.5 3,576,284 + BWI76_RS17250 0.79 -0.1 3,576,711 - -1.3 3,576,797 - +0.5 3,576,815 + +0.2 3,576,823 - -0.7 3,577,054 - -0.3 3,577,203 - BWI76_RS17255 0.28 -0.1 3,577,203 - BWI76_RS17255 0.28 +1.3 3,577,301 - BWI76_RS17255 0.44 -0.4 3,577,313 - BWI76_RS17255 0.46 -0.5 3,577,338 + BWI76_RS17255 0.50 -1.4 3,577,338 + +3.1 3,577,338 + BWI76_RS17255 0.50 +0.3 3,577,338 + BWI76_RS17255 0.50 -2.4 3,577,339 + BWI76_RS17255 0.50 -0.4 3,577,346 - BWI76_RS17255 0.51 -0.6 3,577,346 - BWI76_RS17255 0.51 +0.5 3,577,365 - BWI76_RS17255 0.55 -2.5 3,577,368 - BWI76_RS17255 0.55 -0.4 3,577,376 - BWI76_RS17255 0.56 +0.5 3,577,382 + BWI76_RS17255 0.57 +2.1 3,577,421 - BWI76_RS17255 0.64 +0.4 3,577,421 - BWI76_RS17255 0.64 +0.6 3,577,423 - BWI76_RS17255 0.64 -0.5 3,577,425 + BWI76_RS17255 0.64 -1.4 3,577,433 - BWI76_RS17255 0.66 -0.2 3,577,463 - BWI76_RS17255 0.70 -1.1 3,577,473 + BWI76_RS17255 0.72 +1.3 3,577,481 - BWI76_RS17255 0.73 +0.2 3,577,536 + BWI76_RS17255 0.82 -1.4 3,577,536 + BWI76_RS17255 0.82 -0.1 3,577,536 + BWI76_RS17255 0.82 -1.1 3,577,536 + BWI76_RS17255 0.82 +0.7 3,577,544 - BWI76_RS17255 0.83 +1.2 3,577,544 - BWI76_RS17255 0.83 -1.9 3,577,544 - BWI76_RS17255 0.83 -0.4 3,577,544 - BWI76_RS17255 0.83 +0.9 3,577,544 - BWI76_RS17255 0.83 +0.1 3,577,544 - BWI76_RS17255 0.83 +1.5 3,577,544 - BWI76_RS17255 0.83 +0.1 3,577,544 - BWI76_RS17255 0.83 -0.7 3,577,544 - BWI76_RS17255 0.83 -0.5 3,577,544 - BWI76_RS17255 0.83 +2.0 3,577,544 - BWI76_RS17255 0.83 +0.1 3,577,544 - BWI76_RS17255 0.83 -0.2 3,577,589 - +0.2 3,577,593 + +1.6 3,577,599 - -0.6 3,577,602 - +0.5 3,577,623 - +0.2 3,577,659 - +0.2 3,577,723 + +0.2 3,577,728 + -2.2 3,577,729 - +0.2 3,577,740 - -0.2 3,577,757 - -0.2 3,577,759 - -0.7 3,577,759 - +0.6 3,577,773 - +0.3 3,577,797 + +0.9 3,577,802 - -1.0 3,577,805 - -2.2 3,577,816 - -0.2 3,577,825 + BWI76_RS17260 0.11 -1.1 3,577,840 - BWI76_RS17260 0.16 -1.2 3,577,848 - BWI76_RS17260 0.18 -0.5 3,577,848 - BWI76_RS17260 0.18 -1.4 3,577,915 - BWI76_RS17260 0.39 +0.1 3,577,945 + BWI76_RS17260 0.49 -1.4 3,577,963 - BWI76_RS17260 0.54 -0.7 3,577,963 - BWI76_RS17260 0.54 -1.1 3,577,992 - BWI76_RS17260 0.63 -0.6 3,578,014 - BWI76_RS17260 0.70 -0.9 3,578,118 + +0.6 3,578,119 - -1.9 3,578,123 + -0.2 3,578,123 + -1.7 3,578,522 + BWI76_RS17265 0.78 -1.7
Or see this region's nucleotide sequence