Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and Iron (II) 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12695 and BWI76_RS12700 are separated by 277 nucleotides BWI76_RS12700 and BWI76_RS12705 overlap by 50 nucleotides
BWI76_RS12695: BWI76_RS12695 - zinc transporter ZntB, at 2,646,758 to 2,647,741
_RS12695
BWI76_RS12700: BWI76_RS12700 - hypothetical protein, at 2,648,019 to 2,648,273
_RS12700
BWI76_RS12705: BWI76_RS12705 - ATP-dependent RNA helicase, at 2,648,224 to 2,649,597
_RS12705
Position (kb)
2648
2649 Strain fitness (log2 ratio)
-1
0
1 at 2647.035 kb on - strand, within BWI76_RS12695 at 2647.127 kb on + strand, within BWI76_RS12695 at 2647.398 kb on + strand, within BWI76_RS12695 at 2647.475 kb on - strand, within BWI76_RS12695 at 2647.581 kb on + strand, within BWI76_RS12695 at 2647.706 kb on + strand at 2647.840 kb on + strand at 2647.864 kb on + strand at 2647.872 kb on - strand at 2648.068 kb on + strand, within BWI76_RS12700 at 2648.076 kb on - strand, within BWI76_RS12700 at 2648.396 kb on + strand, within BWI76_RS12705 at 2648.424 kb on - strand, within BWI76_RS12705 at 2648.438 kb on - strand, within BWI76_RS12705 at 2648.438 kb on - strand, within BWI76_RS12705 at 2648.662 kb on + strand, within BWI76_RS12705 at 2648.673 kb on + strand, within BWI76_RS12705 at 2648.815 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and Iron (II) 0.002 mg/ml remove 2,647,035 - BWI76_RS12695 0.28 +1.3 2,647,127 + BWI76_RS12695 0.38 -0.2 2,647,398 + BWI76_RS12695 0.65 +0.2 2,647,475 - BWI76_RS12695 0.73 -0.7 2,647,581 + BWI76_RS12695 0.84 +0.0 2,647,706 + +1.3 2,647,840 + +0.8 2,647,864 + +0.7 2,647,872 - +1.5 2,648,068 + BWI76_RS12700 0.19 -0.0 2,648,076 - BWI76_RS12700 0.22 +0.4 2,648,396 + BWI76_RS12705 0.13 -0.1 2,648,424 - BWI76_RS12705 0.15 +0.3 2,648,438 - BWI76_RS12705 0.16 -0.1 2,648,438 - BWI76_RS12705 0.16 +0.0 2,648,662 + BWI76_RS12705 0.32 +0.1 2,648,673 + BWI76_RS12705 0.33 +0.4 2,648,815 + BWI76_RS12705 0.43 -1.0 2,649,044 + BWI76_RS12705 0.60 -0.5 2,649,044 + BWI76_RS12705 0.60 -0.2
Or see this region's nucleotide sequence