Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09415

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and Iron (II) 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09405 and BWI76_RS09410 overlap by 38 nucleotidesBWI76_RS09410 and BWI76_RS09415 are separated by 74 nucleotidesBWI76_RS09415 and BWI76_RS09420 overlap by 38 nucleotidesBWI76_RS09420 and BWI76_RS09425 are separated by 264 nucleotides BWI76_RS09405: BWI76_RS09405 - hypothetical protein, at 1,972,453 to 1,972,632 _RS09405 BWI76_RS09410: BWI76_RS09410 - reverse transcriptase, at 1,972,595 to 1,973,191 _RS09410 BWI76_RS09415: BWI76_RS09415 - IS3 family transposase, at 1,973,266 to 1,974,377 _RS09415 BWI76_RS09420: BWI76_RS09420 - hypothetical protein, at 1,974,340 to 1,974,579 _RS09420 BWI76_RS09425: BWI76_RS09425 - transporter, at 1,974,844 to 1,976,529 _RS09425 Position (kb) 1973 1974 1975Strain fitness (log2 ratio) -2 -1 0 1 2at 1972.293 kb on - strandat 1972.329 kb on + strandat 1972.331 kb on + strandat 1972.434 kb on - strandat 1972.434 kb on - strandat 1972.434 kb on - strandat 1972.441 kb on + strandat 1972.458 kb on - strandat 1972.477 kb on + strand, within BWI76_RS09405at 1972.477 kb on + strand, within BWI76_RS09405at 1972.509 kb on + strand, within BWI76_RS09405at 1972.509 kb on + strand, within BWI76_RS09405at 1972.534 kb on + strand, within BWI76_RS09405at 1972.544 kb on + strand, within BWI76_RS09405at 1972.570 kb on + strand, within BWI76_RS09405at 1972.638 kb on + strandat 1972.646 kb on + strandat 1972.646 kb on - strandat 1972.657 kb on + strand, within BWI76_RS09410at 1972.665 kb on + strand, within BWI76_RS09410at 1972.665 kb on + strand, within BWI76_RS09410at 1972.673 kb on - strand, within BWI76_RS09410at 1972.691 kb on + strand, within BWI76_RS09410at 1972.770 kb on - strand, within BWI76_RS09410at 1972.881 kb on + strand, within BWI76_RS09410at 1972.974 kb on + strand, within BWI76_RS09410at 1972.974 kb on + strand, within BWI76_RS09410at 1973.007 kb on - strand, within BWI76_RS09410at 1973.019 kb on + strand, within BWI76_RS09410at 1973.049 kb on + strand, within BWI76_RS09410at 1973.107 kb on + strand, within BWI76_RS09410at 1973.161 kb on + strandat 1973.235 kb on - strandat 1974.411 kb on - strand, within BWI76_RS09420at 1974.785 kb on + strandat 1974.785 kb on + strandat 1974.793 kb on - strandat 1974.857 kb on + strandat 1974.923 kb on + strandat 1975.074 kb on + strand, within BWI76_RS09425at 1975.300 kb on - strand, within BWI76_RS09425

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma virens G41 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1 x and Iron (II) 0.002 mg/ml
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1,972,293 - +0.7
1,972,329 + +0.2
1,972,331 + -2.2
1,972,434 - +0.2
1,972,434 - -0.4
1,972,434 - -0.2
1,972,441 + -0.3
1,972,458 - +1.0
1,972,477 + BWI76_RS09405 0.13 -0.5
1,972,477 + BWI76_RS09405 0.13 +0.7
1,972,509 + BWI76_RS09405 0.31 +0.1
1,972,509 + BWI76_RS09405 0.31 +0.4
1,972,534 + BWI76_RS09405 0.45 +0.8
1,972,544 + BWI76_RS09405 0.51 +0.2
1,972,570 + BWI76_RS09405 0.65 +0.3
1,972,638 + -0.3
1,972,646 + +0.3
1,972,646 - -0.9
1,972,657 + BWI76_RS09410 0.10 -0.8
1,972,665 + BWI76_RS09410 0.12 +1.0
1,972,665 + BWI76_RS09410 0.12 -0.2
1,972,673 - BWI76_RS09410 0.13 +0.0
1,972,691 + BWI76_RS09410 0.16 -1.2
1,972,770 - BWI76_RS09410 0.29 +0.6
1,972,881 + BWI76_RS09410 0.48 -0.4
1,972,974 + BWI76_RS09410 0.63 -1.3
1,972,974 + BWI76_RS09410 0.63 -0.4
1,973,007 - BWI76_RS09410 0.69 +0.7
1,973,019 + BWI76_RS09410 0.71 -1.2
1,973,049 + BWI76_RS09410 0.76 -0.3
1,973,107 + BWI76_RS09410 0.86 -1.0
1,973,161 + +2.0
1,973,235 - -0.3
1,974,411 - BWI76_RS09420 0.30 -1.9
1,974,785 + -1.2
1,974,785 + +0.6
1,974,793 - -0.9
1,974,857 + +0.0
1,974,923 + +1.2
1,975,074 + BWI76_RS09425 0.14 +0.2
1,975,300 - BWI76_RS09425 0.27 -1.8

Or see this region's nucleotide sequence