Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3386 and Psest_3387 are separated by 35 nucleotides Psest_3387 and Psest_3388 are separated by 242 nucleotides Psest_3388 and Psest_3389 are separated by 152 nucleotides Psest_3389 and Psest_3390 are separated by 95 nucleotides
Psest_3386: Psest_3386 - ribosomal protein L27, at 3,557,165 to 3,557,422
_3386
Psest_3387: Psest_3387 - ribosomal protein L21, at 3,557,458 to 3,557,769
_3387
Psest_3388: Psest_3388 - Geranylgeranyl pyrophosphate synthase, at 3,558,012 to 3,558,980
_3388
Psest_3389: Psest_3389 - hypothetical protein, at 3,559,133 to 3,559,360
_3389
Psest_3390: Psest_3390 - FKBP-type peptidyl-prolyl cis-trans isomerases 1, at 3,559,456 to 3,560,073
_3390
Position (kb)
3558
3559 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3557.915 kb on - strand at 3559.037 kb on + strand at 3559.037 kb on + strand at 3559.037 kb on + strand at 3559.037 kb on + strand at 3559.083 kb on - strand at 3559.083 kb on - strand at 3559.139 kb on + strand at 3559.140 kb on - strand at 3559.140 kb on - strand at 3559.146 kb on + strand at 3559.147 kb on - strand at 3559.175 kb on + strand, within Psest_3389 at 3559.175 kb on + strand, within Psest_3389 at 3559.186 kb on - strand, within Psest_3389 at 3559.245 kb on + strand, within Psest_3389 at 3559.282 kb on + strand, within Psest_3389 at 3559.337 kb on + strand, within Psest_3389 at 3559.337 kb on + strand, within Psest_3389 at 3559.338 kb on - strand at 3559.338 kb on - strand at 3559.338 kb on - strand at 3559.359 kb on - strand at 3559.416 kb on + strand at 3559.416 kb on + strand at 3559.416 kb on + strand at 3559.416 kb on + strand at 3559.416 kb on + strand at 3559.416 kb on + strand at 3559.417 kb on - strand at 3559.417 kb on - strand at 3559.533 kb on - strand, within Psest_3390 at 3559.533 kb on - strand, within Psest_3390 at 3559.533 kb on - strand, within Psest_3390 at 3559.553 kb on + strand, within Psest_3390 at 3559.553 kb on + strand, within Psest_3390 at 3559.553 kb on + strand, within Psest_3390 at 3559.554 kb on - strand, within Psest_3390 at 3559.702 kb on - strand, within Psest_3390 at 3559.702 kb on - strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.705 kb on + strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.706 kb on - strand, within Psest_3390 at 3559.757 kb on + strand, within Psest_3390 at 3559.758 kb on - strand, within Psest_3390
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,557,915 - +1.2 3,559,037 + -0.2 3,559,037 + +1.0 3,559,037 + +0.4 3,559,037 + +0.6 3,559,083 - +0.7 3,559,083 - -0.0 3,559,139 + +0.4 3,559,140 - -0.8 3,559,140 - +0.8 3,559,146 + -0.0 3,559,147 - -1.2 3,559,175 + Psest_3389 0.18 -0.7 3,559,175 + Psest_3389 0.18 +0.5 3,559,186 - Psest_3389 0.23 +0.4 3,559,245 + Psest_3389 0.49 -0.2 3,559,282 + Psest_3389 0.65 -0.5 3,559,337 + Psest_3389 0.89 +1.1 3,559,337 + Psest_3389 0.89 +0.0 3,559,338 - +0.2 3,559,338 - +0.6 3,559,338 - +0.6 3,559,359 - -0.8 3,559,416 + -1.3 3,559,416 + -0.1 3,559,416 + -2.3 3,559,416 + -0.1 3,559,416 + -1.4 3,559,416 + +1.0 3,559,417 - +0.3 3,559,417 - -0.2 3,559,533 - Psest_3390 0.12 +2.6 3,559,533 - Psest_3390 0.12 +0.8 3,559,533 - Psest_3390 0.12 -0.2 3,559,553 + Psest_3390 0.16 -0.4 3,559,553 + Psest_3390 0.16 -0.6 3,559,553 + Psest_3390 0.16 -1.1 3,559,554 - Psest_3390 0.16 -0.4 3,559,702 - Psest_3390 0.40 -0.3 3,559,702 - Psest_3390 0.40 -1.2 3,559,705 + Psest_3390 0.40 +0.3 3,559,705 + Psest_3390 0.40 -0.2 3,559,705 + Psest_3390 0.40 -0.6 3,559,705 + Psest_3390 0.40 +0.7 3,559,705 + Psest_3390 0.40 +0.2 3,559,705 + Psest_3390 0.40 +1.5 3,559,705 + Psest_3390 0.40 +1.0 3,559,705 + Psest_3390 0.40 -1.6 3,559,705 + Psest_3390 0.40 +1.2 3,559,705 + Psest_3390 0.40 +0.2 3,559,705 + Psest_3390 0.40 -0.2 3,559,705 + Psest_3390 0.40 +0.1 3,559,705 + Psest_3390 0.40 -1.1 3,559,705 + Psest_3390 0.40 -1.7 3,559,706 - Psest_3390 0.40 +0.2 3,559,706 - Psest_3390 0.40 +0.2 3,559,706 - Psest_3390 0.40 -0.8 3,559,706 - Psest_3390 0.40 +0.0 3,559,706 - Psest_3390 0.40 +0.7 3,559,706 - Psest_3390 0.40 +0.3 3,559,706 - Psest_3390 0.40 +0.0 3,559,706 - Psest_3390 0.40 +0.1 3,559,757 + Psest_3390 0.49 -0.1 3,559,758 - Psest_3390 0.49 +0.2
Or see this region's nucleotide sequence