Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2989 and Psest_2990 are separated by 171 nucleotides Psest_2990 and Psest_2991 are separated by 49 nucleotides
Psest_2989: Psest_2989 - alanine--tRNA ligase, at 3,178,397 to 3,181,021
_2989
Psest_2990: Psest_2990 - Threonine aldolase, at 3,181,193 to 3,182,218
_2990
Psest_2991: Psest_2991 - hypothetical protein, at 3,182,268 to 3,183,488
_2991
Position (kb)
3181
3182
3183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3181.134 kb on - strand at 3181.134 kb on - strand at 3181.134 kb on - strand at 3181.134 kb on - strand at 3181.134 kb on - strand at 3181.136 kb on - strand at 3181.378 kb on - strand, within Psest_2990 at 3181.378 kb on - strand, within Psest_2990 at 3181.378 kb on - strand, within Psest_2990 at 3181.496 kb on + strand, within Psest_2990 at 3181.771 kb on - strand, within Psest_2990 at 3181.798 kb on + strand, within Psest_2990 at 3181.798 kb on + strand, within Psest_2990 at 3181.799 kb on - strand, within Psest_2990 at 3181.799 kb on - strand, within Psest_2990 at 3181.799 kb on - strand, within Psest_2990 at 3181.953 kb on + strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.954 kb on - strand, within Psest_2990 at 3181.959 kb on + strand, within Psest_2990 at 3181.959 kb on + strand, within Psest_2990 at 3181.959 kb on + strand, within Psest_2990 at 3181.959 kb on + strand, within Psest_2990 at 3181.964 kb on - strand, within Psest_2990 at 3181.964 kb on - strand, within Psest_2990 at 3181.964 kb on - strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.986 kb on + strand, within Psest_2990 at 3181.987 kb on - strand, within Psest_2990 at 3181.987 kb on - strand, within Psest_2990 at 3181.987 kb on - strand, within Psest_2990 at 3182.035 kb on + strand, within Psest_2990 at 3182.036 kb on - strand, within Psest_2990 at 3182.036 kb on - strand, within Psest_2990 at 3182.036 kb on - strand, within Psest_2990 at 3182.036 kb on - strand, within Psest_2990 at 3182.187 kb on - strand at 3182.201 kb on + strand at 3182.202 kb on - strand at 3182.202 kb on - strand at 3182.409 kb on - strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.590 kb on + strand, within Psest_2991 at 3182.591 kb on - strand, within Psest_2991 at 3182.722 kb on + strand, within Psest_2991 at 3182.722 kb on + strand, within Psest_2991 at 3182.723 kb on - strand, within Psest_2991 at 3182.723 kb on - strand, within Psest_2991 at 3182.894 kb on + strand, within Psest_2991
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,181,134 - -0.6 3,181,134 - +0.3 3,181,134 - +0.5 3,181,134 - +0.4 3,181,134 - +1.2 3,181,136 - +1.4 3,181,378 - Psest_2990 0.18 +1.3 3,181,378 - Psest_2990 0.18 -0.3 3,181,378 - Psest_2990 0.18 +1.0 3,181,496 + Psest_2990 0.30 -1.2 3,181,771 - Psest_2990 0.56 -0.6 3,181,798 + Psest_2990 0.59 -0.1 3,181,798 + Psest_2990 0.59 +0.5 3,181,799 - Psest_2990 0.59 -0.9 3,181,799 - Psest_2990 0.59 +0.3 3,181,799 - Psest_2990 0.59 +0.1 3,181,953 + Psest_2990 0.74 +0.1 3,181,954 - Psest_2990 0.74 -1.1 3,181,954 - Psest_2990 0.74 -2.1 3,181,954 - Psest_2990 0.74 +0.5 3,181,954 - Psest_2990 0.74 -0.0 3,181,954 - Psest_2990 0.74 +0.4 3,181,954 - Psest_2990 0.74 -0.4 3,181,954 - Psest_2990 0.74 -0.3 3,181,954 - Psest_2990 0.74 +2.3 3,181,954 - Psest_2990 0.74 +0.7 3,181,954 - Psest_2990 0.74 +1.3 3,181,954 - Psest_2990 0.74 +0.1 3,181,959 + Psest_2990 0.75 +0.1 3,181,959 + Psest_2990 0.75 +0.7 3,181,959 + Psest_2990 0.75 +0.4 3,181,959 + Psest_2990 0.75 -0.4 3,181,964 - Psest_2990 0.75 +0.6 3,181,964 - Psest_2990 0.75 +0.1 3,181,964 - Psest_2990 0.75 -0.7 3,181,986 + Psest_2990 0.77 +0.3 3,181,986 + Psest_2990 0.77 +0.7 3,181,986 + Psest_2990 0.77 -1.0 3,181,986 + Psest_2990 0.77 +0.5 3,181,986 + Psest_2990 0.77 +0.1 3,181,986 + Psest_2990 0.77 -0.2 3,181,986 + Psest_2990 0.77 -0.0 3,181,987 - Psest_2990 0.77 +0.3 3,181,987 - Psest_2990 0.77 -0.1 3,181,987 - Psest_2990 0.77 -0.1 3,182,035 + Psest_2990 0.82 -0.1 3,182,036 - Psest_2990 0.82 -1.5 3,182,036 - Psest_2990 0.82 +0.1 3,182,036 - Psest_2990 0.82 -0.7 3,182,036 - Psest_2990 0.82 -0.2 3,182,187 - +0.7 3,182,201 + -0.3 3,182,202 - +0.1 3,182,202 - -2.9 3,182,409 - Psest_2991 0.12 +0.2 3,182,590 + Psest_2991 0.26 -1.7 3,182,590 + Psest_2991 0.26 -0.4 3,182,590 + Psest_2991 0.26 -2.3 3,182,590 + Psest_2991 0.26 +0.1 3,182,590 + Psest_2991 0.26 +0.0 3,182,590 + Psest_2991 0.26 -1.3 3,182,590 + Psest_2991 0.26 -0.6 3,182,591 - Psest_2991 0.26 -0.0 3,182,722 + Psest_2991 0.37 -0.2 3,182,722 + Psest_2991 0.37 +0.3 3,182,723 - Psest_2991 0.37 +0.4 3,182,723 - Psest_2991 0.37 +0.2 3,182,894 + Psest_2991 0.51 -0.2
Or see this region's nucleotide sequence