Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2446 and Psest_2447 are separated by 11 nucleotides Psest_2447 and Psest_2448 are separated by 186 nucleotides
Psest_2446: Psest_2446 - acetyl-CoA acetyltransferases, at 2,615,025 to 2,616,209
_2446
Psest_2447: Psest_2447 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,616,221 to 2,616,988
_2447
Psest_2448: Psest_2448 - AraC-type DNA-binding domain-containing proteins, at 2,617,175 to 2,618,242
_2448
Position (kb)
2616
2617 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2615.749 kb on + strand, within Psest_2446 at 2615.836 kb on + strand, within Psest_2446 at 2615.837 kb on - strand, within Psest_2446 at 2615.837 kb on - strand, within Psest_2446 at 2616.171 kb on - strand at 2616.190 kb on - strand at 2616.222 kb on + strand at 2616.223 kb on - strand at 2616.313 kb on - strand, within Psest_2447 at 2616.410 kb on + strand, within Psest_2447 at 2616.508 kb on - strand, within Psest_2447 at 2616.508 kb on - strand, within Psest_2447 at 2616.968 kb on + strand at 2616.968 kb on + strand at 2616.969 kb on - strand at 2616.969 kb on - strand at 2616.969 kb on - strand at 2616.969 kb on - strand at 2617.033 kb on - strand at 2617.225 kb on + strand at 2617.251 kb on + strand at 2617.252 kb on - strand at 2617.526 kb on + strand, within Psest_2448 at 2617.526 kb on + strand, within Psest_2448 at 2617.527 kb on - strand, within Psest_2448 at 2617.680 kb on + strand, within Psest_2448 at 2617.681 kb on - strand, within Psest_2448 at 2617.681 kb on - strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.779 kb on + strand, within Psest_2448 at 2617.780 kb on - strand, within Psest_2448
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,615,749 + Psest_2446 0.61 -1.0 2,615,836 + Psest_2446 0.68 -1.0 2,615,837 - Psest_2446 0.69 -3.6 2,615,837 - Psest_2446 0.69 -1.2 2,616,171 - -0.8 2,616,190 - -0.6 2,616,222 + -0.6 2,616,223 - -0.2 2,616,313 - Psest_2447 0.12 -0.1 2,616,410 + Psest_2447 0.25 -3.5 2,616,508 - Psest_2447 0.37 -1.2 2,616,508 - Psest_2447 0.37 -0.3 2,616,968 + -1.9 2,616,968 + -1.0 2,616,969 - +2.4 2,616,969 - +0.1 2,616,969 - -0.2 2,616,969 - -0.9 2,617,033 - +0.6 2,617,225 + -1.3 2,617,251 + -0.9 2,617,252 - -1.8 2,617,526 + Psest_2448 0.33 -0.7 2,617,526 + Psest_2448 0.33 +0.6 2,617,527 - Psest_2448 0.33 -2.1 2,617,680 + Psest_2448 0.47 -1.0 2,617,681 - Psest_2448 0.47 -0.5 2,617,681 - Psest_2448 0.47 -0.3 2,617,779 + Psest_2448 0.57 -0.4 2,617,779 + Psest_2448 0.57 -1.3 2,617,779 + Psest_2448 0.57 -2.4 2,617,780 - Psest_2448 0.57 -1.1
Or see this region's nucleotide sequence