Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2167 and Psest_2168 are separated by 98 nucleotides Psest_2168 and Psest_2169 are separated by 22 nucleotides Psest_2169 and Psest_2170 overlap by 4 nucleotides
Psest_2167: Psest_2167 - glycogen debranching enzyme GlgX, at 2,319,572 to 2,321,722
_2167
Psest_2168: Psest_2168 - Protein of unknown function (DUF2934)., at 2,321,821 to 2,322,150
_2168
Psest_2169: Psest_2169 - malto-oligosyltrehalose synthase, at 2,322,173 to 2,324,959
_2169
Psest_2170: Psest_2170 - 4-alpha-glucanotransferase, at 2,324,956 to 2,327,031
_2170
Position (kb)
2322
2323
2324
2325 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2321.183 kb on + strand, within Psest_2167 at 2321.183 kb on + strand, within Psest_2167 at 2321.183 kb on + strand, within Psest_2167 at 2321.183 kb on + strand, within Psest_2167 at 2321.183 kb on + strand, within Psest_2167 at 2321.184 kb on - strand, within Psest_2167 at 2321.184 kb on - strand, within Psest_2167 at 2321.184 kb on - strand, within Psest_2167 at 2321.184 kb on - strand, within Psest_2167 at 2321.184 kb on - strand, within Psest_2167 at 2321.184 kb on - strand, within Psest_2167 at 2321.196 kb on + strand, within Psest_2167 at 2321.454 kb on + strand, within Psest_2167 at 2321.469 kb on + strand, within Psest_2167 at 2321.469 kb on + strand, within Psest_2167 at 2321.499 kb on + strand, within Psest_2167 at 2321.500 kb on - strand, within Psest_2167 at 2321.500 kb on - strand, within Psest_2167 at 2321.523 kb on + strand at 2322.175 kb on - strand at 2322.175 kb on - strand at 2322.175 kb on - strand at 2322.175 kb on - strand at 2322.175 kb on - strand at 2322.175 kb on - strand at 2322.178 kb on + strand at 2322.178 kb on + strand at 2322.178 kb on + strand at 2322.179 kb on - strand at 2322.179 kb on - strand at 2322.184 kb on + strand at 2322.185 kb on - strand at 2322.185 kb on - strand at 2322.185 kb on - strand at 2322.185 kb on - strand at 2322.185 kb on - strand at 2322.185 kb on - strand at 2322.185 kb on - strand at 2322.466 kb on + strand, within Psest_2169 at 2322.526 kb on + strand, within Psest_2169 at 2322.526 kb on + strand, within Psest_2169 at 2322.527 kb on - strand, within Psest_2169 at 2322.527 kb on - strand, within Psest_2169 at 2322.687 kb on + strand, within Psest_2169 at 2322.834 kb on + strand, within Psest_2169 at 2322.834 kb on + strand, within Psest_2169 at 2322.834 kb on + strand, within Psest_2169 at 2322.834 kb on + strand, within Psest_2169 at 2322.835 kb on - strand, within Psest_2169 at 2322.883 kb on + strand, within Psest_2169 at 2322.999 kb on - strand, within Psest_2169 at 2323.162 kb on + strand, within Psest_2169 at 2323.163 kb on - strand, within Psest_2169 at 2323.163 kb on - strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.551 kb on + strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.552 kb on - strand, within Psest_2169 at 2323.560 kb on + strand, within Psest_2169 at 2323.631 kb on + strand, within Psest_2169 at 2323.632 kb on - strand, within Psest_2169 at 2323.632 kb on - strand, within Psest_2169 at 2323.632 kb on - strand, within Psest_2169 at 2323.632 kb on - strand, within Psest_2169 at 2323.632 kb on - strand, within Psest_2169 at 2323.905 kb on + strand, within Psest_2169 at 2323.905 kb on + strand, within Psest_2169 at 2323.905 kb on + strand, within Psest_2169 at 2323.906 kb on - strand, within Psest_2169 at 2323.906 kb on - strand, within Psest_2169 at 2324.187 kb on + strand, within Psest_2169 at 2324.247 kb on + strand, within Psest_2169 at 2324.248 kb on - strand, within Psest_2169 at 2324.248 kb on - strand, within Psest_2169 at 2324.248 kb on - strand, within Psest_2169 at 2324.248 kb on - strand, within Psest_2169 at 2324.248 kb on - strand, within Psest_2169 at 2324.248 kb on - strand, within Psest_2169 at 2324.388 kb on - strand, within Psest_2169 at 2324.388 kb on - strand, within Psest_2169 at 2324.457 kb on + strand, within Psest_2169 at 2324.469 kb on + strand, within Psest_2169 at 2324.469 kb on + strand, within Psest_2169 at 2324.470 kb on - strand, within Psest_2169 at 2324.850 kb on + strand at 2324.850 kb on + strand at 2324.850 kb on + strand at 2324.850 kb on + strand at 2324.850 kb on + strand at 2324.850 kb on + strand at 2324.851 kb on - strand at 2324.851 kb on - strand at 2324.851 kb on - strand at 2324.854 kb on + strand at 2324.854 kb on + strand at 2324.880 kb on + strand at 2324.880 kb on + strand at 2325.239 kb on + strand, within Psest_2170 at 2325.239 kb on + strand, within Psest_2170 at 2325.240 kb on - strand, within Psest_2170 at 2325.615 kb on - strand, within Psest_2170 at 2325.615 kb on - strand, within Psest_2170 at 2325.732 kb on - strand, within Psest_2170
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,321,183 + Psest_2167 0.75 -0.7 2,321,183 + Psest_2167 0.75 -0.7 2,321,183 + Psest_2167 0.75 +0.2 2,321,183 + Psest_2167 0.75 -0.4 2,321,183 + Psest_2167 0.75 +0.2 2,321,184 - Psest_2167 0.75 -0.7 2,321,184 - Psest_2167 0.75 -1.6 2,321,184 - Psest_2167 0.75 +0.2 2,321,184 - Psest_2167 0.75 -0.3 2,321,184 - Psest_2167 0.75 -0.3 2,321,184 - Psest_2167 0.75 -0.1 2,321,196 + Psest_2167 0.75 +1.4 2,321,454 + Psest_2167 0.87 +0.7 2,321,469 + Psest_2167 0.88 -0.4 2,321,469 + Psest_2167 0.88 +0.2 2,321,499 + Psest_2167 0.90 -0.8 2,321,500 - Psest_2167 0.90 +1.1 2,321,500 - Psest_2167 0.90 -0.1 2,321,523 + -2.3 2,322,175 - +0.0 2,322,175 - -1.6 2,322,175 - -0.6 2,322,175 - -1.1 2,322,175 - -1.1 2,322,175 - +0.2 2,322,178 + +0.3 2,322,178 + -0.5 2,322,178 + +0.5 2,322,179 - +1.7 2,322,179 - -1.6 2,322,184 + +0.3 2,322,185 - -1.3 2,322,185 - -0.1 2,322,185 - -0.6 2,322,185 - +1.4 2,322,185 - +0.1 2,322,185 - +0.7 2,322,185 - -1.5 2,322,466 + Psest_2169 0.11 +0.7 2,322,526 + Psest_2169 0.13 +0.5 2,322,526 + Psest_2169 0.13 +0.7 2,322,527 - Psest_2169 0.13 +0.5 2,322,527 - Psest_2169 0.13 +1.4 2,322,687 + Psest_2169 0.18 -0.5 2,322,834 + Psest_2169 0.24 -3.2 2,322,834 + Psest_2169 0.24 +0.9 2,322,834 + Psest_2169 0.24 +0.5 2,322,834 + Psest_2169 0.24 +0.5 2,322,835 - Psest_2169 0.24 -0.3 2,322,883 + Psest_2169 0.25 +1.2 2,322,999 - Psest_2169 0.30 -0.8 2,323,162 + Psest_2169 0.35 +0.4 2,323,163 - Psest_2169 0.36 +0.6 2,323,163 - Psest_2169 0.36 -2.4 2,323,551 + Psest_2169 0.49 -0.6 2,323,551 + Psest_2169 0.49 -0.6 2,323,551 + Psest_2169 0.49 +0.4 2,323,551 + Psest_2169 0.49 +1.2 2,323,551 + Psest_2169 0.49 +0.4 2,323,551 + Psest_2169 0.49 +0.3 2,323,551 + Psest_2169 0.49 -0.2 2,323,551 + Psest_2169 0.49 -0.7 2,323,551 + Psest_2169 0.49 -0.2 2,323,551 + Psest_2169 0.49 +0.5 2,323,551 + Psest_2169 0.49 +1.3 2,323,551 + Psest_2169 0.49 -1.4 2,323,551 + Psest_2169 0.49 +2.1 2,323,551 + Psest_2169 0.49 +0.1 2,323,552 - Psest_2169 0.49 +1.1 2,323,552 - Psest_2169 0.49 +0.3 2,323,552 - Psest_2169 0.49 +0.0 2,323,552 - Psest_2169 0.49 +2.7 2,323,552 - Psest_2169 0.49 +1.0 2,323,552 - Psest_2169 0.49 -1.1 2,323,552 - Psest_2169 0.49 +0.2 2,323,552 - Psest_2169 0.49 -1.0 2,323,560 + Psest_2169 0.50 -2.3 2,323,631 + Psest_2169 0.52 +1.0 2,323,632 - Psest_2169 0.52 +0.2 2,323,632 - Psest_2169 0.52 +0.1 2,323,632 - Psest_2169 0.52 -0.8 2,323,632 - Psest_2169 0.52 +0.5 2,323,632 - Psest_2169 0.52 -1.8 2,323,905 + Psest_2169 0.62 +0.7 2,323,905 + Psest_2169 0.62 -0.2 2,323,905 + Psest_2169 0.62 +1.0 2,323,906 - Psest_2169 0.62 +0.9 2,323,906 - Psest_2169 0.62 -1.8 2,324,187 + Psest_2169 0.72 +0.5 2,324,247 + Psest_2169 0.74 +0.2 2,324,248 - Psest_2169 0.74 +0.3 2,324,248 - Psest_2169 0.74 +1.0 2,324,248 - Psest_2169 0.74 +1.1 2,324,248 - Psest_2169 0.74 -0.6 2,324,248 - Psest_2169 0.74 -0.2 2,324,248 - Psest_2169 0.74 -0.1 2,324,388 - Psest_2169 0.79 +0.2 2,324,388 - Psest_2169 0.79 +0.8 2,324,457 + Psest_2169 0.82 +0.9 2,324,469 + Psest_2169 0.82 -1.1 2,324,469 + Psest_2169 0.82 +0.7 2,324,470 - Psest_2169 0.82 +0.1 2,324,850 + +0.2 2,324,850 + +0.4 2,324,850 + -0.4 2,324,850 + +1.2 2,324,850 + -1.3 2,324,850 + -0.2 2,324,851 - +0.2 2,324,851 - -0.3 2,324,851 - +0.5 2,324,854 + -0.3 2,324,854 + +0.6 2,324,880 + -1.3 2,324,880 + -0.4 2,325,239 + Psest_2170 0.14 +0.5 2,325,239 + Psest_2170 0.14 -2.4 2,325,240 - Psest_2170 0.14 -0.3 2,325,615 - Psest_2170 0.32 +2.1 2,325,615 - Psest_2170 0.32 -1.8 2,325,732 - Psest_2170 0.37 -0.0
Or see this region's nucleotide sequence