Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0166 and Psest_0167 are separated by 213 nucleotides Psest_0167 and Psest_0168 are separated by 1 nucleotides Psest_0168 and Psest_0169 are separated by 197 nucleotides Psest_0169 and Psest_0170 are separated by 28 nucleotides
Psest_0166: Psest_0166 - NADPH:quinone reductase and related Zn-dependent oxidoreductases, at 163,588 to 164,568
_0166
Psest_0167: Psest_0167 - Flagellar basal body-associated protein, at 164,782 to 165,183
_0167
Psest_0168: Psest_0168 - Uncharacterized conserved protein, at 165,185 to 165,634
_0168
Psest_0169: Psest_0169 - hypothetical protein, at 165,832 to 165,984
_0169
Psest_0170: Psest_0170 - 5,10-methenyltetrahydrofolate synthetase, at 166,013 to 166,615
_0170
Position (kb)
165
166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.199 kb on - strand, within Psest_0166 at 164.199 kb on - strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.626 kb on - strand at 164.644 kb on + strand at 164.644 kb on + strand at 164.645 kb on - strand at 164.645 kb on - strand at 164.695 kb on + strand at 164.696 kb on - strand at 164.696 kb on - strand at 164.696 kb on - strand at 164.696 kb on - strand at 164.697 kb on + strand at 164.697 kb on + strand at 164.697 kb on + strand at 164.697 kb on + strand at 164.698 kb on - strand at 164.788 kb on + strand at 164.875 kb on + strand, within Psest_0167 at 164.876 kb on - strand, within Psest_0167 at 164.878 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.933 kb on - strand, within Psest_0167 at 164.933 kb on - strand, within Psest_0167 at 164.933 kb on - strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 165.135 kb on + strand, within Psest_0167 at 165.136 kb on - strand, within Psest_0167 at 165.486 kb on + strand, within Psest_0168 at 165.486 kb on + strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.515 kb on - strand, within Psest_0168 at 165.515 kb on - strand, within Psest_0168 at 165.541 kb on - strand, within Psest_0168 at 165.549 kb on - strand, within Psest_0168 at 165.627 kb on + strand at 165.703 kb on + strand at 165.703 kb on + strand at 165.703 kb on + strand at 165.704 kb on - strand at 165.704 kb on - strand at 165.704 kb on - strand at 165.750 kb on - strand at 165.750 kb on - strand at 165.769 kb on + strand at 165.769 kb on + strand at 165.777 kb on + strand at 165.777 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.841 kb on - strand at 165.849 kb on + strand, within Psest_0169 at 165.903 kb on + strand, within Psest_0169 at 166.023 kb on + strand at 166.023 kb on + strand at 166.058 kb on + strand at 166.058 kb on + strand at 166.058 kb on + strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.173 kb on + strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 164,198 + Psest_0166 0.62 +0.8 164,198 + Psest_0166 0.62 +0.2 164,198 + Psest_0166 0.62 +0.1 164,198 + Psest_0166 0.62 -0.1 164,198 + Psest_0166 0.62 +0.2 164,198 + Psest_0166 0.62 -2.1 164,199 - Psest_0166 0.62 -0.6 164,199 - Psest_0166 0.62 -0.9 164,417 + Psest_0166 0.85 +2.3 164,417 + Psest_0166 0.85 +2.0 164,417 + Psest_0166 0.85 +0.2 164,417 + Psest_0166 0.85 +0.3 164,417 + Psest_0166 0.85 +0.5 164,418 - Psest_0166 0.85 +0.4 164,418 - Psest_0166 0.85 +0.5 164,418 - Psest_0166 0.85 -0.2 164,418 - Psest_0166 0.85 -0.5 164,418 - Psest_0166 0.85 -0.7 164,626 - -1.0 164,644 + +0.8 164,644 + +1.1 164,645 - +1.0 164,645 - -0.9 164,695 + +0.2 164,696 - -0.7 164,696 - +1.1 164,696 - -0.6 164,696 - -0.6 164,697 + -1.4 164,697 + +1.0 164,697 + +1.2 164,697 + +1.0 164,698 - +0.2 164,788 + +0.5 164,875 + Psest_0167 0.23 +0.8 164,876 - Psest_0167 0.23 +0.6 164,878 + Psest_0167 0.24 -0.4 164,908 + Psest_0167 0.31 +0.8 164,908 + Psest_0167 0.31 +1.1 164,908 + Psest_0167 0.31 +0.2 164,908 + Psest_0167 0.31 -0.1 164,908 + Psest_0167 0.31 -0.0 164,908 + Psest_0167 0.31 +1.2 164,908 + Psest_0167 0.31 -1.2 164,908 + Psest_0167 0.31 -2.7 164,908 + Psest_0167 0.31 -1.5 164,908 + Psest_0167 0.31 +0.1 164,908 + Psest_0167 0.31 -0.4 164,908 + Psest_0167 0.31 -0.4 164,909 - Psest_0167 0.32 -0.1 164,909 - Psest_0167 0.32 +0.3 164,909 - Psest_0167 0.32 +0.2 164,909 - Psest_0167 0.32 +1.3 164,909 - Psest_0167 0.32 +0.7 164,909 - Psest_0167 0.32 -1.7 164,909 - Psest_0167 0.32 +0.7 164,932 + Psest_0167 0.37 -0.9 164,932 + Psest_0167 0.37 -1.0 164,932 + Psest_0167 0.37 +0.6 164,932 + Psest_0167 0.37 -1.7 164,932 + Psest_0167 0.37 -0.9 164,933 - Psest_0167 0.38 -0.4 164,933 - Psest_0167 0.38 +0.5 164,933 - Psest_0167 0.38 -0.5 164,935 + Psest_0167 0.38 +0.5 164,935 + Psest_0167 0.38 -1.8 164,935 + Psest_0167 0.38 -0.1 164,935 + Psest_0167 0.38 -0.0 164,935 + Psest_0167 0.38 -0.7 164,935 + Psest_0167 0.38 -2.2 164,935 + Psest_0167 0.38 -0.0 164,936 - Psest_0167 0.38 +1.5 164,936 - Psest_0167 0.38 -3.1 164,936 - Psest_0167 0.38 +1.1 164,936 - Psest_0167 0.38 +0.9 164,936 - Psest_0167 0.38 -0.7 164,936 - Psest_0167 0.38 +0.4 164,936 - Psest_0167 0.38 -0.7 164,936 - Psest_0167 0.38 +0.0 164,936 - Psest_0167 0.38 -0.2 164,936 - Psest_0167 0.38 +1.5 165,135 + Psest_0167 0.88 +0.4 165,136 - Psest_0167 0.88 +0.0 165,486 + Psest_0168 0.67 -1.1 165,486 + Psest_0168 0.67 -1.5 165,487 - Psest_0168 0.67 -1.8 165,487 - Psest_0168 0.67 +0.7 165,487 - Psest_0168 0.67 -2.5 165,487 - Psest_0168 0.67 -0.3 165,487 - Psest_0168 0.67 -0.6 165,514 + Psest_0168 0.73 +0.9 165,514 + Psest_0168 0.73 -2.2 165,514 + Psest_0168 0.73 +1.0 165,515 - Psest_0168 0.73 +0.3 165,515 - Psest_0168 0.73 +1.8 165,541 - Psest_0168 0.79 +0.7 165,549 - Psest_0168 0.81 +0.6 165,627 + +0.3 165,703 + -2.2 165,703 + +0.5 165,703 + -0.9 165,704 - +1.0 165,704 - -0.2 165,704 - +1.4 165,750 - +0.6 165,750 - +0.1 165,769 + +0.8 165,769 + -0.7 165,777 + +2.7 165,777 + -2.5 165,840 + +0.8 165,840 + +0.3 165,840 + +1.7 165,840 + +0.3 165,841 - +0.6 165,849 + Psest_0169 0.11 -0.4 165,903 + Psest_0169 0.46 -2.8 166,023 + +0.9 166,023 + -0.2 166,058 + +0.2 166,058 + +0.7 166,058 + -0.9 166,059 - -1.1 166,059 - -2.4 166,059 - +0.6 166,059 - -0.5 166,059 - -1.0 166,173 + Psest_0170 0.27 +0.7 166,174 - Psest_0170 0.27 +0.6 166,174 - Psest_0170 0.27 +0.0 166,174 - Psest_0170 0.27 +0.5 166,174 - Psest_0170 0.27 -0.0 166,174 - Psest_0170 0.27 -0.8 166,174 - Psest_0170 0.27 +0.5 166,174 - Psest_0170 0.27 -0.7 166,174 - Psest_0170 0.27 +0.0 166,383 + Psest_0170 0.61 -1.0 166,383 + Psest_0170 0.61 -0.1 166,383 + Psest_0170 0.61 +2.1 166,383 + Psest_0170 0.61 -0.1 166,383 + Psest_0170 0.61 -1.9 166,384 - Psest_0170 0.62 +0.2 166,384 - Psest_0170 0.62 -2.0 166,384 - Psest_0170 0.62 +0.4 166,456 - Psest_0170 0.73 +1.0 166,456 - Psest_0170 0.73 +0.7 166,456 - Psest_0170 0.73 +0.7
Or see this region's nucleotide sequence