Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1036 and Psest_1037 are separated by 2 nucleotides Psest_1037 and Psest_1038 are separated by 76 nucleotides Psest_1038 and Psest_1039 are separated by 153 nucleotides
Psest_1036: Psest_1036 - acetolactate synthase, large subunit, biosynthetic type, at 1,121,522 to 1,123,246
_1036
Psest_1037: Psest_1037 - acetolactate synthase, small subunit, at 1,123,249 to 1,123,740
_1037
Psest_1038: Psest_1038 - ketol-acid reductoisomerase, at 1,123,817 to 1,124,833
_1038
Psest_1039: Psest_1039 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,124,987 to 1,125,826
_1039
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1123.512 kb on + strand, within Psest_1037 at 1123.512 kb on + strand, within Psest_1037 at 1123.596 kb on + strand, within Psest_1037 at 1123.608 kb on + strand, within Psest_1037 at 1123.727 kb on + strand at 1123.734 kb on + strand at 1123.734 kb on + strand at 1123.734 kb on + strand at 1123.734 kb on + strand at 1123.735 kb on - strand at 1123.735 kb on - strand at 1123.738 kb on + strand at 1123.747 kb on + strand at 1124.902 kb on + strand at 1124.962 kb on + strand at 1124.962 kb on + strand at 1124.962 kb on + strand at 1124.971 kb on + strand at 1124.971 kb on + strand at 1124.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,123,512 + Psest_1037 0.53 -0.6 1,123,512 + Psest_1037 0.53 -0.6 1,123,596 + Psest_1037 0.71 -3.5 1,123,608 + Psest_1037 0.73 -2.3 1,123,727 + +0.9 1,123,734 + +3.9 1,123,734 + -1.6 1,123,734 + +0.5 1,123,734 + -1.8 1,123,735 - +1.1 1,123,735 - +1.1 1,123,738 + +1.8 1,123,747 + +0.1 1,124,902 + -2.1 1,124,962 + +0.6 1,124,962 + +0.2 1,124,962 + -0.2 1,124,971 + +1.0 1,124,971 + +0.7 1,124,971 + -0.2
Or see this region's nucleotide sequence