Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0568 and Psest_0569 are separated by 21 nucleotides Psest_0569 and Psest_0570 are separated by 174 nucleotides Psest_0570 and Psest_0571 are separated by 160 nucleotides Psest_0571 and Psest_0573 are separated by 544 nucleotides
Psest_0568: Psest_0568 - pseudouridine synthase, at 625,010 to 625,639
_0568
Psest_0569: Psest_0569 - Protein of unknown function (DUF2788)., at 625,661 to 625,879
_0569
Psest_0570: Psest_0570 - hypothetical protein, at 626,054 to 626,446
_0570
Psest_0571: Psest_0571 - Transcriptional regulators, at 626,607 to 627,086
_0571
Psest_0573: Psest_0573 - cation diffusion facilitator family transporter, at 627,631 to 628,521
_0573
Position (kb) 626
627
628 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 625.638 kb on - strand at 625.944 kb on - strand at 625.944 kb on - strand at 625.944 kb on - strand at 625.945 kb on + strand at 625.945 kb on + strand at 625.945 kb on + strand at 625.945 kb on + strand at 625.945 kb on + strand at 625.946 kb on - strand at 625.946 kb on - strand at 625.946 kb on - strand at 625.946 kb on - strand at 625.946 kb on - strand at 626.160 kb on - strand, within Psest_0570 at 626.181 kb on + strand, within Psest_0570 at 626.181 kb on + strand, within Psest_0570 at 626.181 kb on + strand, within Psest_0570 at 626.182 kb on - strand, within Psest_0570 at 626.197 kb on + strand, within Psest_0570 at 626.373 kb on + strand, within Psest_0570 at 626.373 kb on + strand, within Psest_0570 at 626.373 kb on + strand, within Psest_0570 at 626.373 kb on + strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.374 kb on - strand, within Psest_0570 at 626.415 kb on + strand at 626.416 kb on - strand at 626.558 kb on + strand at 626.559 kb on - strand at 626.560 kb on + strand at 626.560 kb on + strand at 626.560 kb on + strand at 626.560 kb on + strand at 626.560 kb on + strand at 626.561 kb on - strand at 626.561 kb on - strand at 626.582 kb on + strand at 626.582 kb on + strand at 626.764 kb on + strand, within Psest_0571 at 626.765 kb on - strand, within Psest_0571 at 626.881 kb on + strand, within Psest_0571 at 626.939 kb on + strand, within Psest_0571 at 626.940 kb on - strand, within Psest_0571 at 626.940 kb on - strand, within Psest_0571 at 626.941 kb on + strand, within Psest_0571 at 626.941 kb on + strand, within Psest_0571 at 626.941 kb on + strand at 626.941 kb on + strand, within Psest_0571 at 626.941 kb on + strand, within Psest_0571 at 626.941 kb on + strand, within Psest_0571 at 626.941 kb on + strand, within Psest_0571 at 626.941 kb on + strand, within Psest_0571 at 626.942 kb on - strand, within Psest_0571 at 626.942 kb on - strand, within Psest_0571 at 626.942 kb on - strand, within Psest_0571 at 626.942 kb on - strand, within Psest_0571 at 626.942 kb on - strand, within Psest_0571 at 627.129 kb on - strand at 627.131 kb on + strand at 627.157 kb on + strand at 627.157 kb on + strand at 627.206 kb on + strand at 627.207 kb on - strand at 627.207 kb on - strand at 627.274 kb on + strand at 627.360 kb on + strand at 627.360 kb on + strand at 627.361 kb on - strand at 627.528 kb on + strand at 627.529 kb on - strand at 627.529 kb on - strand at 627.804 kb on + strand, within Psest_0573 at 627.950 kb on + strand, within Psest_0573 at 627.950 kb on + strand, within Psest_0573 at 627.950 kb on + strand, within Psest_0573 at 627.950 kb on + strand, within Psest_0573 at 627.950 kb on + strand, within Psest_0573 at 627.950 kb on + strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 627.951 kb on - strand, within Psest_0573 at 628.055 kb on + strand, within Psest_0573
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 625,638 - -1.3 625,944 - +1.2 625,944 - -0.2 625,944 - -1.7 625,945 + +1.2 625,945 + +1.1 625,945 + -0.1 625,945 + +0.5 625,945 + -0.1 625,946 - -1.6 625,946 - +1.1 625,946 - +0.1 625,946 - -1.7 625,946 - +0.5 626,160 - Psest_0570 0.27 -0.1 626,181 + Psest_0570 0.32 -1.3 626,181 + Psest_0570 0.32 +1.7 626,181 + Psest_0570 0.32 -0.9 626,182 - Psest_0570 0.33 +1.2 626,197 + Psest_0570 0.36 +0.9 626,373 + Psest_0570 0.81 +1.4 626,373 + Psest_0570 0.81 -1.7 626,373 + Psest_0570 0.81 +0.7 626,373 + Psest_0570 0.81 +0.5 626,374 - Psest_0570 0.81 +1.7 626,374 - Psest_0570 0.81 -1.8 626,374 - Psest_0570 0.81 +1.2 626,374 - Psest_0570 0.81 -0.4 626,374 - Psest_0570 0.81 -2.6 626,374 - Psest_0570 0.81 +0.4 626,374 - Psest_0570 0.81 -1.0 626,415 + +0.5 626,416 - +0.1 626,558 + +1.5 626,559 - -2.7 626,560 + -0.2 626,560 + +0.2 626,560 + +0.7 626,560 + -1.4 626,560 + -1.9 626,561 - -1.3 626,561 - +0.0 626,582 + -1.9 626,582 + +2.6 626,764 + Psest_0571 0.33 -0.6 626,765 - Psest_0571 0.33 +0.5 626,881 + Psest_0571 0.57 -2.8 626,939 + Psest_0571 0.69 -0.7 626,940 - Psest_0571 0.69 -0.9 626,940 - Psest_0571 0.69 +0.4 626,941 + Psest_0571 0.70 -0.9 626,941 + Psest_0571 0.70 -0.3 626,941 + -1.9 626,941 + Psest_0571 0.70 -2.0 626,941 + Psest_0571 0.70 -1.1 626,941 + Psest_0571 0.70 -1.9 626,941 + Psest_0571 0.70 -1.3 626,941 + Psest_0571 0.70 +0.3 626,942 - Psest_0571 0.70 -1.3 626,942 - Psest_0571 0.70 +0.9 626,942 - Psest_0571 0.70 -0.3 626,942 - Psest_0571 0.70 -0.1 626,942 - Psest_0571 0.70 -1.3 627,129 - -1.0 627,131 + -1.1 627,157 + -0.5 627,157 + -1.0 627,206 + +0.2 627,207 - -2.5 627,207 - -3.3 627,274 + +0.7 627,360 + -0.6 627,360 + -0.8 627,361 - +0.2 627,528 + +0.7 627,529 - -0.1 627,529 - -2.3 627,804 + Psest_0573 0.19 -2.1 627,950 + Psest_0573 0.36 -0.1 627,950 + Psest_0573 0.36 -0.6 627,950 + Psest_0573 0.36 +0.2 627,950 + Psest_0573 0.36 -0.2 627,950 + Psest_0573 0.36 +0.2 627,950 + Psest_0573 0.36 +1.9 627,951 - Psest_0573 0.36 +0.6 627,951 - Psest_0573 0.36 +0.1 627,951 - Psest_0573 0.36 +2.3 627,951 - Psest_0573 0.36 +0.5 627,951 - Psest_0573 0.36 +0.6 627,951 - Psest_0573 0.36 +1.2 627,951 - Psest_0573 0.36 +0.1 628,055 + Psest_0573 0.48 +2.9
Or see this region's nucleotide sequence